DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-alpha180 and POL1

DIOPT Version :9

Sequence 1:	NP_536736.2	Gene:	DNApol-alpha180 / 42553	FlyBaseID:	FBgn0259113	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_014297.3	Gene:	POL1 / 855621	SGDID:	S000005046	Length:	1468	Species:	Saccharomyces cerevisiae

Alignment Length:	1548	Identity:	488/1548 - (31%)
Similarity:	767/1548 - (49%)	Gaps:	209/1548 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    24 MERLRQLKGTKNKCKVEDQVDD------VYDVVDEREY-AKRAQEKYGDDW-IEEDGTGYA---- 76
            :|:||:|:..:|...::|...|      :||.:||:|| |::.||...||: :::||.||.
Yeast     8 LEKLRKLQAARNGTSIDDYEGDESDGDRIYDEIDEKEYRARKRQELLHDDFVVDDDGVGYVDRGV 72

  Fly    77 -EDLRDFFEDEDEYSDGEEDRKDSKKKKGVAPNSKKRPRENEKPVTG----KASIKNLFSNAVPK 136
             ||.|:......:...|....||||:||.:       .||.:..:|.    :.|...|.::|
Yeast    73 EEDWREVDNSSSDEDTGNLASKDSKRKKNI-------KREKDHQITDMLRTQHSKSTLLAHA--- 127

  Fly   137 KMDVKTSVKDDDILADILGEIKEEPAATSEKAEK--VIAPAKI------SVTSR----------- 182
            |...|.|:..|: ..|||||.:      |.:.||  ::.|:|:      |.||.
Yeast   128 KKSQKKSIPIDN-FDDILGEFE------SGEVEKPNILLPSKLRENLNSSPTSEFKSSIKRVNGN 185

  Fly   183 ---KFDAAAAK-----------EYMNSFLNNIKVQEQERKKAEASSDNEMLERILKPKAAVPNTK 233
               ..||..:|           :|:....:.:|:  |.||...|:...::|:       .|.|:.
Yeast   186 DESSHDAGISKKVKIDPDSSTDKYLEIESSPLKL--QSRKLRYANDVQDLLD-------DVENSP 241

  Fly   234 VAFFSSPTIKKEPMPEKTPAKKATEDPFSDNEMDFSCLDDDENQFDVEKTQQTEKVSQTKTAAEK 298
            |.......:.::.:....|:.::    .:|.|      ||:::..|:...::|.:...|......
Yeast   242 VVATKRQNVLQDTLLANPPSAQS----LADEE------DDEDSDEDIILKRRTMRSVTTTRRVNI 296

  Fly   299 TSQSKVAEKSAPKKETTGSPKESESEDISRLLNNWESICQMDDDFEKSVLTTEQDSTISSDQQLR 363
            .|:|.  ..::|.....|:|...:....|:.|.:...:..:..:.:|..:...:..|..     .
Yeast   297 DSRSN--PSTSPFVTAPGTPIGIKGLTPSKSLQSNTDVATLAVNVKKEDVVDPETDTFQ-----M 354

  Fly   364 FWYWEAYEDPVKMPGEVFLFGRT--ADGKSVCLRVQNIN---RVLYLLPRQFLLDPISKEPTKQK 423
            ||.     |..::...:.|||:.  .|...|...|| ||   |.|:.|||:      .|.||
Yeast   355 FWL-----DYCEVNNTLILFGKVKLKDDNCVSAMVQ-INGLCRELFFLPRE------GKTPT--- 404

  Fly   424 VTVADIYKEFDSEVANQLKLEFFRSR--KVTKSFAHHAIGIEVPQSCDYLEVHYDGKKP------ 480
                ||::|....:.::..|:..|::  |:..||...    ::|...|||:|....:.|
Yeast   405 ----DIHEEIIPLLMDKYGLDNIRAKPQKMKYSFELP----DIPSESDYLKVLLPYQTPKSSRDT 461

  Fly   481 LP-NLSADKKYNSIAHIFGATTNALERFLLDRKIKGPCWLQVTGFKV-SPTPMSWCNTEVTLTEP 543
            :| :||:|..|    |:||..:|..|.|::..:|.|||||.:.|... |....|.|..||::.:|
Yeast   462 IPSDLSSDTFY----HVFGGNSNIFESFVIQNRIMGPCWLDIKGADFNSIRNASHCAVEVSVDKP 522

  Fly   544 KNVELVQDKGKPAPPPPLTLLSLNVRTSMNPKTSRNEICMISMLTHNRFHIDRPAPQPAFNRHMC 608
            :|:.....|    ..|.|..|||:::|.||||.::.||..|::..:....:|.|.|:......:|
Yeast   523 QNITPTTTK----TMPNLRCLSLSIQTLMNPKENKQEIVSITLSAYRNISLDSPIPENIKPDDLC 583

  Fly   609 ALTR-PAVVSWPLDLNFEMAKYK-STTVHKHDSERALLSWFLAQYQKIDADLIVTFDSMDCQLNV 671
            .|.| |...|:||.| ..:||.| ...|...::|:|:||.|.|..:..|.|:|:.....:..|:|
Yeast   584 TLVRPPQSTSFPLGL-AALAKQKLPGRVRLFNNEKAMLSCFCAMLKVEDPDVIIGHRLQNVYLDV 647

  Fly   672 ITDQIVALKIPQWSRMGRLRLSQSF------GKRLLEHFV------GRMVCDVKRSAEECI--RA 722
            :..::..|.||.:|.:|| ||.:::      |...:.||.      ||::||:.....:.:  :.
Yeast   648 LAHRMHDLNIPTFSSIGR-RLRRTWPEKFGRGNSNMNHFFISDICSGRLICDIANEMGQSLTPKC 711

  Fly   723 RSYDLQTLCKQVLKLKESERMEVNADDLLEMYEKGESITKLISLTM--QDNSYLLRLMCE----L 781
            :|:||..: .||...||.:.::::       |:..:....:.|:||  |:|.....:..|    :
Yeast   712 QSWDLSEM-YQVTCEKEHKPLDID-------YQNPQYQNDVNSMTMALQENITNCMISAEVSYRI 768

  Fly   782 NIMPLALQITNICGNTMTRTLQGGRSERNEFLLLHAFHEKNYIVPDKKPVSKRSGAGDTDATLSG 846
            .::.|..|:||:.||...:||.|.|:.|||::|||.|....:|||||:  ..||.|.........
Yeast   769 QLLTLTKQLTNLAGNAWAQTLGGTRAGRNEYILLHEFSRNGFIVPDKE--GNRSRAQKQRQNEEN 831

  Fly   847 ADATMQTKKKAAYAGGLVLEPMRGLYEKYVLLMDFNSLYPSIIQEYNICFTTV-QQPVDADELPT 910
            |||.:.: |||.|.||||.||.:||::.|||:|||||||||||||:||||||| :...|.||||:
Yeast   832 ADAPVNS-KKAKYQGGLVFEPEKGLHKNYVLVMDFNSLYPSIIQEFNICFTTVDRNKEDIDELPS 895

  Fly   911 LPDSKTEPGILPLQLKRLVESRKEVKKLMAAPDLSPELQMQYHIRQMALKLTANSMYGCLGFAHS 975
            :|.|:.:.|:||..|..||:.|:||||:|.. :..|..::|..|||.||||||||||||||:.:|
Yeast   896 VPPSEVDQGVLPRLLANLVDRRREVKKVMKT-ETDPHKRVQCDIRQQALKLTANSMYGCLGYVNS 959

  Fly   976 RFFAQHLAALVTHKGREILTNTQQLVQKMNYDVVYGDTDSLMINTNITDYDQVYKIGHNIKQSVN 1040
            ||:|:.||.|||:||||||.||:||.:.||..||||||||:||:|...:|....|||...|:.||
Yeast   960 RFYAKPLAMLVTNKGREILMNTRQLAESMNLLVVYGDTDSVMIDTGCDNYADAIKIGLGFKRLVN 1024

  Fly  1041 KLYKQLELDIDGVFGCLLLLKKKKYAAIKLSKDSKGNLRREQEHKGLDIVRRDWSQLAVMVGKAV 1105
            :.|:.||:|||.||..|||..||||||:.::.|..||.....|.||||:.||::..|:..|...|
Yeast  1025 ERYRLLEIDIDNVFKKLLLHAKKKYAALTVNLDKNGNGTTVLEVKGLDMKRREFCPLSRDVSIHV 1089

  Fly  1106 LDEVLSEKPLEEKLDAVHAQLEKIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALR 1170
            |:.:||:|..||.|..|:..||.|:.::....:.:..:.|..:|::.|:.|....::|.||||||
Yeast  1090 LNTILSDKDPEEALQEVYDYLEDIRIKVETNNIRIDKYKINMKLSKDPKAYPGGKNMPAVQVALR 1154

  Fly  1171 MNRERNRRYKKGDMVDYVIC-LDGTTNAA-------MQRAYHLDELKTSEDKKLQLDTNYYLGHQ 1227
            | |:..|..|.|.::.:||. .|...|||       .:||:.|:|:....:..:. |..|||..|
Yeast  1155 M-RKAGRVVKAGSVITFVITKQDEIDNAADTPALSVAERAHALNEVMIKSNNLIP-DPQYYLEKQ 1217

  Fly  1228 IHPVVTRMVEVLEGTDASRIAECLGMDPTK-FRQNAQRTQRENTEQSEGESLLKTTLQLYRLCEP 1291
            |...|.|::|.::..:..|::|.||:|..| ||:.......|:....:......|.::.::....
Yeast  1218 IFAPVERLLERIDSFNVVRLSEALGLDSKKYFRREGGNNNGEDINNLQPLETTITDVERFKDTVT 1282

  Fly  1292 FRFQCVTCKTE----QLMASAYRPGPSNSHIAVLQQCAKSECQTAPIQYLASVRNQLQLSMRQYV 1352
            ....|.:|...    .:::|.|.....|.     .||...|....|:|    :.:|::.|:|.::
Yeast  1283 LELSCPSCDKRFPFGGIVSSNYYRVSYNG-----LQCKHCEQLFTPLQ----LTSQIEHSIRAHI 1338

  Fly  1353 QRFYKNWLVCDHPDCNFNTRTHSLRKKSHRPLCQKCRSGSLLRQYTERDLYNQLCYLRFMFDLGK 1417
            ..:|..||.||...|...||..|:..|  |.|...| :|.:..:|:::.|||||.|...:||..|
Yeast  1339 SLYYAGWLQCDDSTCGIVTRQVSVFGK--RCLNDGC-TGVMRYKYSDKQLYNQLLYFDSLFDCEK 1400

  Fly  1418 QTLQQ-KPTLTP-ELEQAYQLLYETVDQQLQSSSYVIISLSKLFARSLAQMSL 1468
            ...|: ||...| :|:...:.|.|:..:.|...:..::..    .||:.|..|
Yeast  1401 NKKQELKPIYLPDDLDYPKEQLTESSIKALTEQNRELMET----GRSVVQKYL 1449

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-alpha180	NP_536736.2	DNA_pol_alpha_N	24..83	CDD:289058	25/71 (35%)
		pol2	52..1237	CDD:273159	418/1274 (33%)
		DNA_polB_alpha_exo	567..789	CDD:99819	64/243 (26%)
		POLBc_alpha	852..1254	CDD:99915	192/410 (47%)
		zf-DNA_Pol	1286..1459	CDD:286132	45/178 (25%)
POL1	NP_014297.3	pol2	29..1227	CDD:273159	422/1287 (33%)
POL1	NP_014297.3	zf-DNA_Pol	1269..1463	CDD:401082	50/197 (25%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346698
Domainoid	1	1.000	352	1.000	Domainoid score	I140
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H6802
Inparanoid	1	1.050	580	1.000	Inparanoid score	I147
Isobase	1	0.950	-	0	Normalized mean entropy	S1805
OMA	1	1.010	-	-		QHG55031
OrthoFinder	1	1.000	-	-		FOG0004596
OrthoInspector	1	1.000	-	-		oto99342
orthoMCL	1	0.900	-	-		OOG6_102343
Panther	1	1.100	-	-	LDO	PTHR45861
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R343
SonicParanoid	1	1.000	-	-		X3211
TreeFam	1	0.960	-	-
	16	15.740