Protein Alignment PolA1 and POL2

DIOPT Version :10

• Sequence 1: NP_536736.2
• Gene: PolA1 / 42553
• FlyBaseID: FBgn0259113
• Length: 1488
• Species: Drosophila melanogaster
• Sequence 2: NP_014137.1
• Gene: POL2 / 855459
• SGDID: S000005206
• Length: 2222
• Species: Saccharomyces cerevisiae
• Alignment Length: 1455
• Identity: 257/1455 - (17%)
• Similarity: 464/1455 - (31%)
• Gaps: 525/1455 - (36%)

  Fly   384 GRTADGKSVCLRVQNINRV-LYLLPRQ-------FLLDPISKEPTKQKVTVADIYKEFDSEVANQ 440
            |.:.||:.|... |.|:.| .|.|..:       .:.||........:..|.|:.:.....:.:.
Yeast   100 GNSNDGERVTTN-QGISGVDFYFLDEEGGSFKSTVVYDPYFFIACNDESRVNDVEELVKKYLESC 163

  Fly   441 LK-LEFFRSRKVTKSFAHHAIGIE---VPQSCDYLEVHYDGKKPLPNLSADKKYNSI-AHIFGAT 500
            || |:..|...:|..  :|.:|::   :..|.......::.:|.|..:..|...|:: .:|:...
Yeast   164 LKSLQIIRKEDLTMD--NHLLGLQKTLIKLSFVNSNQLFEARKLLRPILQDNANNNVQRNIYNVA 226

  Fly   501 TNALE--------------------RFLLDRKIKGPCWLQVT--GFKVSPTPMSWCNTEVTLTEP 543
            .|..|                    |..:|:.|:...|.:||  ||
Yeast   227 ANGSEKVDAKHLIEDIREYDVPYHVRVSIDKDIRVGKWYKVTQQGF------------------- 272

  Fly   544 KNVELVQDKGKPAPPPPLTLLSLNVRTSMN----PKTSRNEICMIS-------MLTHNRFHIDR- 596
                 ::|..|.|...|: :::.::.|:..    |.::.::|.|||       .|..||..|..
Yeast   273 -----IEDTRKIAFADPV-VMAFDIETTKPPLKFPDSAVDQIMMISYMIDGEGFLITNREIISED 331

  Fly   597 -------PAPQ-PAF------NRHMCAL---------TRPAVVS--------WP----------L 620
                   |.|: |.|      |..:..|         .||.|:|        ||          |
Yeast   332 IEDFEYTPKPEYPGFFTIFNENDEVALLQRFFEHIRDVRPTVISTFNGDFFDWPFIHNRSKIHGL 396

  Fly   621 DLNFEM-------AKYKSTTVHKHDSERALLSW-----FLAQ--------------YQ--KIDAD 657
            |:..|:       .:|||:.....|..|    |     :|.|              |.  ::|.:
Yeast   397 DMFDEIGFAPDAEGEYKSSYCSHMDCFR----WVKRDSYLPQGSQGLKAVTQSKLGYNPIELDPE 457

  Fly   658 LIVTFDSMDCQLNVITDQIVALKIPQWSRMGRLRLSQSFG-----KRLLEHFVGRMVCDVKRSAE 717
            |:..:               |.:.||  .:....:|.:..     .:.:..|:..:...:..:.:
Yeast   458 LMTPY---------------AFEKPQ--HLSEYSVSDAVATYYLYMKYVHPFIFSLCTIIPLNPD 505

  Fly   718 ECIRARSYDLQTLCKQVL-------------------------KLKESE---------------- 741
            |.:|..:   .|||:.:|                         .|.|||
Yeast   506 ETLRKGT---GTLCEMLLMVQAYQHNILLPNKHTDPIERFYDGHLLESETYVGGHVESLEAGVFR 567

  Fly   742 -------RMEVNA-DDLLE----------MYEKGESITKLIS------------LTMQDN----- 771
                   :::.:| |:||:          ..|...|:.|:.:            |.:::|
Yeast   568 SDLKNEFKIDPSAIDELLQELPEALKFSVEVENKSSVDKVTNFEEIKNQITQKLLELKENNIRNE 632

  Fly   772 ----------SYLLRLMCELNIMPLALQITNIC--------GNTMTRTLQGGRSERNEF------ 812
                      |....:|....:.|.:::....|        |.|..|.|:.  :.|.||
Yeast   633 LPLIYHVDVASMYPNIMTTNRLQPDSIKAERDCASCDFNRPGKTCARKLKW--AWRGEFFPSKMD 695

  Fly   813 ---LLLHAFHEKNYIVPDKKPVSKRSGAGDTDATLSGADATMQTKKKAAYAGGLVLEPMRGLYEK 874
               ::..|...:.:  |:|...||:...  |...||.||..:..||:       :.|..|.:|.:
Yeast   696 EYNMIKRALQNETF--PNKNKFSKKKVL--TFDELSYADQVIHIKKR-------LTEYSRKVYHR 749

  Fly   875 YVLLMDFNSLYPSIIQEYNICFTTVQQPVDADELPTLPD--------SKTEPGILPLQLKRLVES 931
            ..:         |.|.|........:.|...|.:.:..|        :||..|    .|.::..|
Yeast   750 VKV---------SEIVEREAIVCQRENPFYVDTVKSFRDRRYEFKGLAKTWKG----NLSKIDPS 801

  Fly   932 ----RKEVKKLMAAPDLSPELQMQYHIRQMALKLTANSMYGCLGFAHSRFFAQHLAALVTHKGRE 992
                |.|.||::          :.|...|:|.|:..||.||.:....||:::..:|.:....|..
Yeast   802 DKHARDEAKKMI----------VLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLTGAT 856

  Fly   993 ILTNTQQLVQKMNYDVVYGDTDSL-----------------------------MINTNI----TD 1024
            |:...:.||:::...:.. |||.:                             |:|..:    |:
Yeast   857 IIQMARALVERVGRPLEL-DTDGIWCILPKSFPETYFFTLENGKKLYLSYPCSMLNYRVHQKFTN 920

  Fly  1025 YDQVYKIGHNIKQSVNKLYK-----QLELDIDGVFGCLLLLK--------KKKYAAIKLSKDSKG 1076
            :..     ..:|..:|.:|:     .:..::||.:..::|..        ||:||..    :..|
Yeast   921 HQY-----QELKDPLNYIYETHSENTIFFEVDGPYKAMILPSSKEEGKGIKKRYAVF----NEDG 976

  Fly  1077 NLRREQEHKGLDIVRRDWSQLAVMVGKAVLDEVLSEKPLE--------------EKLDAVHAQLE 1127
            :|   .|.||.::.||...||.......:....|....||              :.||:....||
Yeast   977 SL---AELKGFELKRRGELQLIKNFQSDIFKVFLEGDTLEGCYSAVASVCNRWLDVLDSHGLMLE 1038

  Fly  1128 KIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALRMN--RERNRRYKKGDMVDYVI- 1189
            .      |.:|  .|....:.:::|.:||....| ..:..|.|:.  ...:....||....|:|
Yeast  1039 D------EDLV--SLICENRSMSKTLKEYEGQKS-TSITTARRLGDFLGEDMVKDKGLQCKYIIS 1094

  Fly  1190 ------------------CLDGTTNAAMQRAYHLDELKTSEDKKLQLDTNYY---LGHQIHPVVT 1233
                              ..|.....:..|.:.||......|.:..:|..||   ||..|..::|
Yeast  1095 SKPFNAPVTERAIPVAIFSADIPIKRSFLRRWTLDPSLEDLDIRTIIDWGYYRERLGSAIQKIIT 1159

  Fly  1234 RMVEVLEGTD--------ASRIAECLGMDPTKFRQNA-----QRTQRENT--EQSEGESLLKTTL 1283
             :...|:|..        ...:...:.....||:|.:     .:|:...|  :..:.|.|.:.|:
Yeast  1160 -IPAALQGVSNPVPRVEHPDWLKRKIATKEDKFKQTSLTKFFSKTKNVPTMGKIKDIEDLFEPTV 1223

  Fly  1284 QLYRLCEPFRFQCVTCKTEQLMASAYRPGPSNSHIAVLQQCAKSECQTAPIQYLASVRNQLQL-S 1347
            :                            ..|:.|.:.:...|..........|.:..:.|.| |
Yeast  1224 E----------------------------EDNAKIKIARTTKKKAVSKRKRNQLTNEEDPLVLPS 1260

  Fly  1348 MRQYVQRFYKNWLVCDHPDCNFNTR-----------THSLRKKSHRPLCQKCRSGSLLRQYTERD 1401
            ....:...|..||...........|           |:|.|::|        ..||::|:..|
Yeast  1261 EIPSMDEDYVGWLNYQKIKWKIQARDRKRRDQLFGNTNSSRERS--------ALGSMIRKQAE-- 1315

  Fly  1402 LYNQLCYLRFMFDLGKQTLQQKPTLTPELEQAYQLLYETVDQQLQSSSYVI------------IS 1454
                 .|....:::    ||.|.:..|.:.:    ::.|::.::|:.::.|            :.
Yeast  1316 -----SYANSTWEV----LQYKDSGEPGVLE----VFVTINGKVQNITFHIPKTIYMKFKSQTMP 1367

  Fly  1455 LSK----LFARSLAQMSLQPSVAQP 1475
            |.|    |..:|.|.:...|..:.|
Yeast  1368 LQKIKNCLIEKSSASLPNNPKTSNP 1392

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PolA1	NP_536736.2	pol2	52..1237	CDD:273159	213/1172 (18%)
		zf-DNA_Pol	1286..1459	CDD:462651	29/200 (15%)
POL2	NP_014137.1	DNA_pol_B_exo1	121..442	CDD:397292	65/351 (19%)
		DNA_polB_epsilon_exo	283..486	CDD:99822	41/224 (18%)
		POLBc_epsilon	545..1166	CDD:99918	127/679 (19%)
		DUF1744	1528..1920	CDD:462493