DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-alpha180 and rev3l

DIOPT Version :9

Sequence 1:	NP_536736.2	Gene:	DNApol-alpha180 / 42553	FlyBaseID:	FBgn0259113	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	XP_002665692.3	Gene:	rev3l / 569100	ZFINID:	ZDB-GENE-050302-55	Length:	2953	Species:	Danio rerio

Alignment Length:	1675	Identity:	338/1675 - (20%)
Similarity:	573/1675 - (34%)	Gaps:	511/1675 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 SPS-EPRAKRQRVDKNGRFAAMERLRQLKGTKNK--------------------------CKVED 41
            ||| :||..|.:|             |.:..|||                          |.|||
Zfish  1466 SPSRKPRGARTKV-------------QTRCKKNKQDDQRNDCLSSDGSPVFFSDPGFDSCCSVED 1517

  Fly    42 ----QVDDVY----DVVDEREYA------------KRAQEKYGDDWIEEDG---TGYAEDLRDFF 83
                ::.|.|    :|:.:.|::            |...:|:..|..:|..   .|.....:..|
Zfish  1518 SLSPELPDNYRFDINVIGQTEFSSLYSGNQFVLTDKILPQKFLSDVSQEAANALVGLGNRTQKMF 1582

  Fly    84 EDEDEYSDGEEDRK-----------------------------DSKK------------------ 101
            :.:|:::.|.:..|                             ||:|
Zfish  1583 DVDDDFNHGNDRNKSGALSPELFDKVSCENGRVFPNKVSLSLLDSEKIFSTNWAGSLGKIHGLSH 1647

  Fly   102 -------KK-------------------GVAPNSKKRPRENEKPVTGKASIKNLFSNAVPKKMDV 140
                   |:                   ||:|||......:..|.:...||.:|        ..:
Zfish  1648 FRDFHCEKRDVLLDPEPKSPIMSASFAYGVSPNSDLLDGSSLTPSSSPRSINSL--------SQL 1704

  Fly   141 KTS-------VKD-------DDILADILG-----EIKEEPAATSEKAEKVIAPAKISVTSRKFDA 186
            |||       :|.       ::|||:::.     ..|:|| ..|:.::..:.|.::.......:.
Zfish  1705 KTSRTGGTRILKPLMSPPAREEILANLMDLDFSEATKQEP-FYSDPSDVPLKPREVGGRKLILET 1768

  Fly   187 AAAKEYMNSFLNNIKVQEQERKKAEASSDNEMLERILKPKAAVPNTKVAFFSSPTIKKEP----M 247
            ..|...: .|..::.....:..|...|:   |..:...|.....::|:....||:...:|    :
Zfish  1769 RQASRLV-EFHGDLSQNGLQFWKVAFSA---MTNQASAPTQGCKSSKLIKNQSPSSVSDPKVIIL 1829

  Fly   248 PEKTPAKKATEDPFSDNEMDFSCLDDDENQFDVEKTQQTEKVSQTKTAAEKTSQSKVAEKSAPKK 312
            |.|....:.....::..:..:.||.....|....:..:.::.:..::..:|||.|..:|....:.
Zfish  1830 PCKCAPSREQVQLWAQAKKQYECLQRGRIQMGGHEISELKQSTCDRSTTQKTSNSLFSENHDKRP 1894

  Fly   313 ETTGSPKESESEDISRLLNNW------------------ESI-CQMDDDFEKSV----------L 348
            |     |.|||:..||  |::                  .|: |..|.|..|..          :
Zfish  1895 E-----KNSESQHNSR--NDFCSPIHFSPVKAASSDSSPSSVKCVQDIDKTKDCRITSPPNSPEI 1952

  Fly   349 TTEQDSTISSDQQLRFWYWEAYEDPVKMPGEVFLFGRTADGKSVCLRVQNINRVL--------YL 405
            :|.|||....:|.         ||...:.|          |.......||:|..|        :|
Zfish  1953 STWQDSPDEVEQD---------EDKENLTG----------GLQPLSMDQNLNETLLENAESKDHL 1998

  Fly   406 LPRQF-------------LL--DPIS-KEPTKQKVTVADIYKEFDSEVANQLKLEFFRSRKVTKS 454
            .|..|             ||  .|:. :..|..:.:.....:..|:|..:|...:  ||.|...:
Zfish  1999 SPSFFGIKGLVSDVRCSHLLHSTPVQRRNSTDDEASSCSPVRHEDTECRSQRLHQ--RSNKNKNA 2061

  Fly   455 FAHHAIGIEVPQSCDYLEVHYDGKKPLPNLSADKKYNSIAHIFGATTNALERFLLDRKIKGPCWL 519
            .....:..:|             |..|.|::..||.||                   :|:||.:.
Zfish  2062 LRRVLLSTQV-------------KNQLINMNVPKKENS-------------------QIEGPSFC 2094

  Fly   520 QVTGFKVSPTPMSWCNTEVTLTEPKNVELVQDKGKPAPPPPLTLLSLNV----RTSMNPKTSRNE 580
            ...|||||..         .|.|.|.:..||.         |||:|:.:    |.::.|....:.
Zfish  2095 NSYGFKVSMQ---------NLQEAKALHEVQH---------LTLMSMELHARTRRNLEPDPEFDP 2141

  Fly   581 ICMI------SMLTHNRFHIDRPAPQPAFNRHMCA-----------LTRPAVVSWPLDLNF---E 625
            ||.:      .:|.|:. ...|.......::..|.           |.|..|...|:....   |
Zfish  2142 ICALFYCLSSDVLLHDS-GTTRIEGAIVIDKESCTSAQASRSSTPFLFRCGVTGLPVTYTCDEKE 2205

  Fly   626 MAKYKSTTVHKHDS------ERALLSW--FLAQYQKIDADLIVTFDSM--DCQLNVITDQIVALK 680
            :.:..|..:.|:|.      |..:.||  .|.:...:..:|......:  |.|.|..
Zfish  2206 LFEEVSNIIRKYDPDILVGYEVQMHSWGYLLQRASTLGVNLCQQLSRVPGDSQQNHF-------- 2262

  Fly   681 IPQWSRMGRLRLSQSFGKRLLEHFVGRMVCDVKRSAEECIRARSYDLQT----LCKQVLKLKESE 741
            ||:....|...:::.       |..||::.::.|..:......:|..:.    |..|...|....
Zfish  2263 IPEKDEYGAETMTEI-------HITGRIILNIWRIMKTEAALNNYTFENVAFHLLHQRYPLYSHR 2320

  Fly   742 RMEVNADDLLEMYEKGESITKLISLTMQDNSYLLRLMCELNIMPLALQITNICGNTMTRTLQGGR 806
            .:....|..|..::..:.....:..|:|       |:.:.:|:....::..:.|......|..|.
Zfish  2321 TLSDWFDTHLHRWKVMDHYVSRVCGTVQ-------LLQQQDIIGRTSELARVFGIQFYHVLTRGS 2378

  Fly   807 SERNEFLLLHAFHEKNYIVPDKKPVSKRSGAGDTDATLSGADATMQTKKKAAYAGGLVLEPMRGL 871
            ..|.|.::|......|||     ||        |.:|      ..:.:::||....||:||....
Zfish  2379 QYRVESMMLRVAKPMNYI-----PV--------TPST------QQRAQQRAAQCIPLVMEPESRF 2424

  Fly   872 YEKYVLLMDFNSLYPSIIQEYNICFTT--------------------VQQPVD-----ADELPTL 911
            |...|:::||.||||||:..||.|::|                    ::.|.|     .:::...
Zfish  2425 YSNSVIVLDFQSLYPSIVIAYNYCYSTCLGHIENLGTYDEFKFGCASLRVPPDLLYQLRNDITVS 2489

  Fly   912 PD------SKTEPGILPLQLKRLVESRKEVKKLMAAPDLSPELQMQYHIRQMALKLTANSMYGCL 970
            |:      |....|:||..|:.::::|..||:.|.|......|......||:.|||.||..:
Zfish  2490 PNGVAFVKSSVRRGVLPRMLEEILKTRIMVKQSMKAYRNDKALSRLLDARQLGLKLIANVTF--- 2551

  Fly   971 GFAHSRFFAQ----HLAALVTHKGREILTNTQQLV---QKMNYDVVYGDTDSLMINTNITDYDQV 1028
            |:..:.|..:    .:...:.||.||.|....::|   :|....||||||||:.:.......:|.
Zfish  2552 GYTSANFSGRMPCVEVGDSIVHKARETLERAIKMVNDTKKWGARVVYGDTDSMFVLLKGATKEQA 2616

  Fly  1029 YKIGHNIKQSVNKLY-KQLELDIDGVF-GCLLLLKKKKYAAIKLSKDSKGNLRREQEHKGLDIVR 1091
            :|||:.|.::|.... |.::|..:.|: .|:|..||:....:..|.|.:..:   .:.||::.||
Zfish  2617 FKIGNEIAEAVTATNPKPIKLKFEKVYLPCILQTKKRYVGYMYESVDQENPV---FDAKGIETVR 2678

  Fly  1092 RDWSQLAVMVGKAVLDEVL--SEKPLEEKLD--AVHAQLEKIKTQIAEGVVPLPLFVITKQLTRT 1152
            ||        |...:.::|  |.|.|.|..|  .|...:::...::.||...:......|:. |.
Zfish  2679 RD--------GCPGVSKILERSLKLLFESRDISQVKQYVQRQCVKVVEGKASMQDLTFAKEY-RG 2734

  Fly  1153 PQEYANSASLPHVQVALRMNR-ERNRRYKKGDMVDYVICLDGTTNAAMQRAYHLDELKTSEDKKL 1216
            ...|...|.:|.:::..||.. :|....:.|:.|.|||.........:|....  .|:..:|..|
Zfish  2735 SGSYRPGACVPALELTRRMMAFDRRLEPRVGERVPYVIVYGSPGVPLIQLVRR--PLEVLQDPSL 2797

  Fly  1217 QLDTNYYLGHQIHPVVTRMVEVLEGTDASRIAECLGMDPTKFRQNAQRTQRENTEQSEG---ESL 1278
            :|:..||:..||.|.:            :||...:|:|...:.....|.||..:..|.|   |..
Zfish  2798 RLNATYYITKQILPPL------------ARIFNLIGVDVFSWYHELPRVQRAWSFTSSGRGEEGT 2850

  Fly  1279 LKTTLQLY------RLCEPFR--FQCVTCKTE--------------------QLM-------ASA 1308
            .|.|:..|      .:|:...  ..|..|:.|                    ||:       .||
Zfish  2851 KKGTISQYFTTLHCPVCDELTQLGVCARCRAEPQCVAVHLHQDLRLWESQQDQLLKICRSCSGSA 2915

  Fly  1309 YRPGPSNSHIAVLQQCAKSECQTAPIQY-LASVRNQLQLS--MRQYVQRF 1355
            .|..|          |...:|   ||.| |:.|...|...  :||.|::|
Zfish  2916 ERQVP----------CVSLDC---PILYKLSRVNRNLSRGPYLRQLVEQF 2952

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-alpha180	NP_536736.2	DNA_pol_alpha_N	24..83	CDD:289058	17/107 (16%)
		pol2	52..1237	CDD:273159	285/1431 (20%)
		DNA_polB_alpha_exo	567..789	CDD:99819	41/259 (16%)
		POLBc_alpha	852..1254	CDD:99915	115/446 (26%)
		zf-DNA_Pol	1286..1459	CDD:286132	23/108 (21%)
rev3l	XP_002665692.3	DUF4683	710..1099	CDD:292363
		DNA_polB_zeta_exo	2116..2350	CDD:99821	44/265 (17%)
		POLBc_zeta	2377..2825	CDD:99917	127/495 (26%)
		DNA_pol_B	2377..2820	CDD:278562	125/490 (26%)
		zf-C4pol	2864..2931	CDD:290959	14/79 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810