DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-alpha180 and pola1

DIOPT Version :9

Sequence 1:	NP_536736.2	Gene:	DNApol-alpha180 / 42553	FlyBaseID:	FBgn0259113	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_001292393.1	Gene:	pola1 / 317740	ZFINID:	ZDB-GENE-030114-9	Length:	1468	Species:	Danio rerio

Alignment Length:	1522	Identity:	601/1522 - (39%)
Similarity:	887/1522 - (58%)	Gaps:	159/1522 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 RAKRQRVDKNGRFAAMERL-RQLKGTKNKCKVEDQVDDVYDVVDEREYAKRAQEKYGDDW-IEED 71
            |::|::.:|.||.:|:|:| |..||.|.|.:|| :...:||.|||.:|:|..:|:..||| |::|
Zfish    28 RSRREKKEKVGRKSALEQLKRAKKGEKVKYEVE-EFTSIYDEVDEEQYSKIVRERQDDDWIIDDD 91

  Fly    72 GTGYAEDLRDFFEDE-DEYSDGEEDRKDSK---KKKGVAPNSKKRPRENEKPVTGKASIKNLFSN 132
            ||||.||.|:.|::| |:.:.|.:.::.:|   .||.|..:|..:|.          |||::|.|
Zfish    92 GTGYVEDGREIFDEELDDDALGPKSKQAAKGGDSKKNVKKSSVSKPN----------SIKSMFMN 146

  Fly   133 A-VPKKMDVKTSVKDDDILADILGEIKEEPAATSEKAEKVIAPAKISVTSRKFDAAAAKEYMNSF 196
            : |.|..:....:..||:|.|||.::..|.:.       |:.|..| :|.:|      |:.:.|.
Zfish   147 SNVKKPAEKHVDLSQDDLLGDILQDLHSEKSV-------VLTPPPI-ITLKK------KKTLGSP 197

  Fly   197 LNNIKVQEQERKKAEASSDNEMLE-RILKPKAAVPNTKVAFFSSPTIKKEPMPEKTPAKKATEDP 260
            :|...|:.|..|        |:.| .:.||:             |.:.:.|..:.:|...|:.:|
Zfish   198 MNPFSVKPQMPK--------ELPEVHVSKPR-------------PVVTRVPPADPSPRSAASVEP 241

  Fly   261 FSD----------NEMDFSCLDDDENQFDVEKTQQTEKVSQTKTAAEKTSQSKVAEKSAPK-KET 314
            ...          .|.:...::.||..||            ....||...:..|.||:.|: |..
Zfish   242 SKATAAQIKKPKIEEPEEEAIEFDEMDFD------------EPMEAENLEEEPVKEKAEPETKPI 294

  Fly   315 TGSPKESESED---ISRLLN--NWESICQMDDDFEKSVLTTEQDSTISS----------DQQLRF 364
            .....::|.:|   ||.|:.  .||   :|::.  |.:.|..:....||          |...||
Zfish   295 VKVEPKTEPQDKMLISGLVGGMGWE---KMEEG--KPIETPVEVQVDSSQLPLVEGPDGDMVFRF 354

  Fly   365 WYWEAYEDPVKMPGEVFLFGR----TADGK-SVCLRVQNINRVLYLLPRQFLLDPISKEPTKQKV 424
            ::.:|:||....||.|||||:    :|:.. |.|:.|:||.|.:|||||:..::..:.| ....|
Zfish   355 YWLDAFEDQYNQPGVVFLFGKVWIESAEAHVSCCVAVKNIERTMYLLPREHKVNLATGE-KGVAV 418

  Fly   425 TVADIYKEFDSEVANQLKLEFFRSRKVTKSFAHHAIGIEVPQSCDYLEVHYDGKKPLPNLSADKK 489
            .:.|:|||| :|::.:.|:..|:|:.|||::|...  .::|...:||||.|..:  :|:|.:|.|
Zfish   419 GMMDVYKEF-NEISEKFKIMKFKSKPVTKNYAFEI--PDIPSQSEYLEVKYSAE--IPSLPSDLK 478

  Fly   490 YNSIAHIFGATTNALERFLLDRKIKGPCWLQVTGFKVSPTPMSWCNTEVTLTEPKNVELVQDKGK 554
            ..:.:|:||..|::||.|||:|||:|||||.||..::|..|:|||..|....:...:.:|:|
Zfish   479 GTTFSHVFGTNTSSLEHFLLNRKIRGPCWLDVTTPQLSNQPISWCKVEAVAQKSDLISVVKD--- 540

  Fly   555 PAPPPPLTLLSLNVRTSMNPKTSRNEICMISMLTHNRFHIDRPAPQPAFNRHMCALTRPAVVSWP 619
             ..||||.::|::::|..||||.:|||..|:.|.|:.|.:|:..|:|.|..|.|.:::|....:|
Zfish   541 -LSPPPLVVMSISLKTVQNPKTHQNEIVSIAALVHHTFPLDKAPPRPPFQTHFCVVSKPNDCVFP 604

  Fly   620 LDLNFEMAKYKSTTVHKHDSERALLSWFLAQYQKIDADLIVTFDSMDCQLNVITDQIVALKIPQW 684
            .|.. :..:.|:..|....:|||||.:|||:..|||.|::|..|.....|.|:..:|...|:|.|
Zfish   605 YDFK-DAVRKKNGIVEIAGTERALLGFFLAKMHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHW 668

  Fly   685 SRMGRLRLS--------QSFGKRLLEHFVGRMVCDVKRSAEECIRARSYDLQTLCKQVLKLKESE 741
            |::||||.:        ..|.::  ....||:|||::.||:|.||.:||:|..|..|:||   :|
Zfish   669 SKIGRLRRANMPKLGGRSGFAEK--SATCGRLVCDIEISAKELIRCKSYNLTELVSQILK---AE 728

  Fly   742 RMEVNADDLLEMYEKGESITKLISLTMQDNSYLLRLMCELNIMPLALQITNICGNTMTRTLQGGR 806
            |..|..:::...|.....:..|:.||..|...:|::|||||::|||||||||.||.|:|||.|||
Zfish   729 RPVVPPENIRNFYSDSTHLLYLLELTWMDAKLILQIMCELNVLPLALQITNIAGNVMSRTLMGGR 793

  Fly   807 SERNEFLLLHAFHEKNYIVPDKKPVSK-RSGAGDTDATLSGADATMQTKKKAAYAGGLVLEPMRG 870
            |||||:||||||||:::|||||:...| :...|:.:...||...:.:|:|||||||||||:|..|
Zfish   794 SERNEYLLLHAFHERDFIVPDKQFFKKAQQDLGEDEEGDSGKGKSNKTRKKAAYAGGLVLDPKVG 858

  Fly   871 LYEKYVLLMDFNSLYPSIIQEYNICFTTVQQPV---------DADELPTLPDSKTEPGILPLQLK 926
            .|:|::||:||||||||||||:|||||||::..         |.||:|.|||...|.||||.:::
Zfish   859 FYDKFILLLDFNSLYPSIIQEFNICFTTVERGATNTRKKTEEDEDEIPELPDQSLEMGILPKEIR 923

  Fly   927 RLVESRKEVKKLMAAPDLSPELQMQYHIRQMALKLTANSMYGCLGFAHSRFFAQHLAALVTHKGR 991
            :|||.|::||:||..|||:|:|.:||.|||.|||||||||||||||:.|||:|:.||||||||||
Zfish   924 KLVERRRQVKQLMKEPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGR 988

  Fly   992 EILTNTQQLVQKMNYDVVYGDTDSLMINTNITDYDQVYKIGHNIKQSVNKLYKQLELDIDGVFGC 1056
            |||.:|:::||:||.:|:||||||:|||||.|:.::|:|:|:.:|..||||||.||:||||||..
Zfish   989 EILMHTKEMVQRMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGVFKS 1053

  Fly  1057 LLLLKKKKYAAIKLSKDSKGNLRREQEHKGLDIVRRDWSQLAVMVGKAVLDEVLSEKPLEEKLDA 1121
            |||||||||||:.:.....|....:||.||||||||||..||...|..|:.::||::..:..::.
Zfish  1054 LLLLKKKKYAALMVEPQGDGKYTTKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQNRDTIIEN 1118

  Fly  1122 VHAQLEKIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALRMNRERNRRYKKGDMVD 1186
            :...|.:|..::|.|.:||.:|.|.|.||:.||:|.:..|||||.|||.:|.:..::.|.||.|.
Zfish  1119 IQKHLIEIGEKVANGNIPLNMFEIHKSLTKEPQDYPDKKSLPHVHVALWINSQGGKKVKAGDTVS 1183

  Fly  1187 YVICLDGTTNAAMQRAYHLDELKTSEDKKLQLDTNYYLGHQIHPVVTRMVEVLEGTDASRIAECL 1251
            ||||.||:..||.||||.|::|:  :...|.|||.|||..|:||||.|:.|.:||.||..||..|
Zfish  1184 YVICQDGSNLAASQRAYTLEQLQ--KQPGLNLDTQYYLSQQVHPVVGRICEPIEGIDAVLIATWL 1246

  Fly  1252 GMDPTKFRQNAQRTQRENTEQSEGESLLKTTLQL-----YRLCEPFRFQCVTCKTEQLMASAYRP 1311
            |:||:.||  ||:.|||:    |.:..|....||     ||.||.|.|.|..|.||.:..:.:..
Zfish  1247 GLDPSHFR--AQQQQRED----EADDFLGAPAQLTDEERYRDCERFTFTCPDCGTENIYDNVFEG 1305

  Fly  1312 GPSNSHIAVLQQCAKSECQTAPIQYLASVRNQLQLSMRQYVQRFYKNWLVCDHPDCNFNTRTHSL 1376
            ..|...:: |:.|....|..:|:.:...:.|:|||.:|::::|:|..||:|:...|....|...:
Zfish  1306 TGSTLQLS-LKSCCHVPCGGSPMDHQVQIGNKLQLDIRRHIRRYYSGWLLCEDQACRNRIRRLPI 1369

  Fly  1377 RKKSHRPLCQKCRSGSLLRQYTERDLYNQLCYLRFMFD---------LGKQTLQQKPTLTPELEQ 1432
            ......|:|..|...:|..:|:|:.|||||.:.|::||         .|...|:.|.....|..:
Zfish  1370 AFSRSGPICPACLRSTLKPEYSEKALYNQLSFYRYIFDWEYAINKVLTGDDKLKFKSKEWKEEME 1434

  Fly  1433 AYQLLYETVDQQLQSSSYVIISLSKLF 1459
            .|:.|....|..|.:|.|..::|:|||
Zfish  1435 VYKKLKGVTDNILATSGYSEVNLAKLF 1461

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-alpha180	NP_536736.2	DNA_pol_alpha_N	24..83	CDD:289058	29/60 (48%)
		pol2	52..1237	CDD:273159	505/1240 (41%)
		DNA_polB_alpha_exo	567..789	CDD:99819	83/229 (36%)
		POLBc_alpha	852..1254	CDD:99915	228/410 (56%)
		zf-DNA_Pol	1286..1459	CDD:286132	53/181 (29%)
pola1	NP_001292393.1	DNA_pol_alpha_N	43..103	CDD:289058	29/60 (48%)
		pol2	58..1232	CDD:273159	511/1254 (41%)
		DNA_polB_alpha_exo	544..776	CDD:99819	87/237 (37%)
		POLBc_alpha	840..1249	CDD:99915	228/410 (56%)
		zf-DNA_Pol	1272..1461	CDD:286132	55/189 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170596507
Domainoid	1	1.000	492	1.000	Domainoid score	I359
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H6802
Inparanoid	1	1.050	996	1.000	Inparanoid score	I291
OMA	1	1.010	-	-		QHG55031
OrthoDB	1	1.010	-	-		D293315at2759
OrthoFinder	1	1.000	-	-		FOG0004596
OrthoInspector	1	1.000	-	-		oto41175
orthoMCL	1	0.900	-	-		OOG6_102343
Panther	1	1.100	-	-	LDO	PTHR45861
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X3211
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.770