DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-alpha180 and pol1

DIOPT Version :9

Sequence 1:	NP_536736.2	Gene:	DNApol-alpha180 / 42553	FlyBaseID:	FBgn0259113	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_594186.1	Gene:	pol1 / 2543629	PomBaseID:	SPAC3H5.06c	Length:	1405	Species:	Schizosaccharomyces pombe

Alignment Length:	1462	Identity:	450/1462 - (30%)
Similarity:	721/1462 - (49%)	Gaps:	164/1462 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 RAKRQRVDKNGRFAAMERLRQLKGTKNKCKVEDQVDDVYDVVDEREYAKRAQEKYGDD--WIEED 71
            |.:...:..:.|||.:..|||...|:.......:.:::||.|.|.||.|..:::..:|  .::::
pombe     2 RKRNAGIKPSQRFAELRALRQAGKTRASTYESKENEELYDNVSEEEYRKIVRQRLDEDDFVVDDN 66

  Fly    72 GTGYAEDLRDFFEDEDEYSDGEEDRKDSKKKKGVAPNSKKRPRENEKPVTGKAS--IKNLF-SNA 133
            |.||.::..|.: |:..|||     :|.:.:||    |..|.|:..|.|..:.:  |...| :||
pombe    67 GAGYVDNGYDEW-DQSHYSD-----EDDENEKG----SSGRKRKKSKNVLAEPNQQIGAFFKANA 121

  Fly   134 VPKKMDV---KTSVKDDDILADILGEIKEEPAATSEKAEKVIAPAKISVT--SRKFDAAAAKEYM 193
            :.....:   |.:.|:|:.:|:|||.|.::            .|.::|..  :|....:.||
pombe   122 IQPASRIANRKNNEKEDEFMAEILGSIDQD------------IPERLSTKKGNRSHTTSNAK--- 171

  Fly   194 NSFLNNIKVQEQERKKAEASSDNEMLERILKPKAAVPNTKVAFFSSP-TIKKEPMPEKTPAKKAT 257
                      .:.:|.|.:.|...::..  .||..:|.|..   |:| .....|:|...||...|
pombe   172 ----------RRSQKAASSISTTNVVSS--DPKKYIPETVP---STPQPASSLPIPSSPPAALET 221

  Fly   258 EDPFSDNEMDFSCLDD-------DENQFDVEKTQQTEKVSQTKTAAEKTSQSKVAEKSAPKKETT 315
            |:......|:   ||:       |....|||::...:.:.......:..|.||......|....|
pombe   222 EENVDSQPME---LDEPELPVGSDAPIIDVEESIAMKPLMSHDQVDDTASLSKATISQQPLSPMT 283

  Fly   316 GSPKESESEDISRLLNNWESICQMDDDFEKSVL------TTEQDSTISSDQQLRFWYWEAYEDPV 374
            ....|.:....|.:.:..:::......:...:.      .||:|.::       |::|..|   .
pombe   284 PLSSELDVSAFSEINSKIKNVDVPASSYSSPISKVSPSDVTEEDGSL-------FFFWMDY---T 338

  Fly   375 KMPGEVFLFGRTADGK-----SVCLRVQNINRVLYLLPRQFLLDPISKEPTKQKVT--VADIYKE 432
            :|.|.:.|||:..|..     |..|:|..|.|.||.|||     |.|...::..:.  ..|:|
pombe   339 EMYGSLCLFGKVYDKATKQYVSCFLKVDGIMRSLYFLPR-----PSSSSVSEDSIAAQTKDVY-- 396

  Fly   433 FDSEVANQLKLEFFRSRKVTKSFAHHAIGIE-VPQSCDYLEVHYDGKKPLPNLSADKKYNSIAHI 496
              .||||.|.....:..|...|...:|..:| ||::.|||||.|  ....|.|..|...:|.:|:
pombe   397 --DEVANLLSKRGVKEWKSRVSKYKYAFELEDVPRTADYLEVIY--SYSYPALPTDLTGSSFSHV 457

  Fly   497 FGATTNALERFLLDRKIKGPCWLQVTGFKVSPT-----PMSWCNTEVTLTEPKNVELVQDKGK-P 555
            ||..|...|:|:|.|::.|||||::.    .|.     ..|||..|:..:.|:|:.:..:|.: .
pombe   458 FGTNTALFEQFVLSRRVMGPCWLKIQ----QPNFDAVKNASWCRVEIGCSSPQNISVSFEKNEIT 518

  Fly   556 APPPPLTLLSLNVRTSMNPKTSRNEICMISMLTHNRFHIDRPAPQPAFNRHMCALTRPAVVSWPL 620
            :..||:|::||..||.:|.:.::.|:.|||........|::..|......:..:|.||....:|
pombe   519 SKTPPMTVMSLAFRTLINKEQNKQEVVMISARIFENVDIEKGLPANDMPSYSFSLIRPLKQIFP- 582

  Fly   621 DLNFE-MAKYKSTTVHKHDSERALLSWFLAQYQKIDADLIVTFDSMDCQLNVITDQIVALKIPQW 684
             ..|| :|:...:::....||.:||:.||.:.:..|.|:....|...| .:|:..::...||..|
pombe   583 -NGFEKLARQHKSSIFCERSEVSLLNNFLNKVRTYDPDVYFGHDFEMC-YSVLLSRLKERKIHNW 645

  Fly   685 SRMGRLRLSQ---SFGKRLLEHFV------GRMVCDVKRS-AEECIRARSYDLQTLCKQVLKLKE 739
            |.:||||.|:   || .|..:.||      ||::||:... ....|:|:|:.|..:   |||..:
pombe   646 SSIGRLRRSEWPRSF-NRSSQQFVEKQIIAGRLMCDLSNDFGRSMIKAQSWSLSEI---VLKELD 706

  Fly   740 SERMEVNADDLLEMY-EKGESITKLISLTMQDNSYLLRLMCELNIMPLALQITNICGNTMTRTLQ 803
            .:|.::|.:..|:.: :....:...:.....|..::..:..::.::.|:..:|||.||:..|||.
pombe   707 IKRQDINQEKALQSWTDTAHGLLDYLVHCEIDTFFIAAVAFKIQMLQLSKNLTNIAGNSWARTLT 771

  Fly   804 GGRSERNEFLLLHAFHEKNYIVPDKKPVSKRSGAGDTDATLSGADATMQT----KKKAAYAGGLV 864
            |.|:||||::|||.|.:..||||||:...:|      .|...||:..:|.    |||..|.||||
pombe   772 GTRAERNEYILLHEFKKNGYIVPDKQQSIRR------HAEAFGAEDGLQEESLGKKKDKYKGGLV 830

  Fly   865 LEPMRGLYEKYVLLMDFNSLYPSIIQEYNICFTTV-QQPVDA---DELPTLPDSKTEPGILPLQL 925
            .||.:||||..:|:||||||||||||||||||||| :.|.::   |::|..|.:....||.|..:
pombe   831 FEPQKGLYETCILVMDFNSLYPSIIQEYNICFTTVDRSPSNSDSDDQIPDTPSASANQGIFPRLI 895

  Fly   926 KRLVESRKEVKKLMAAPDLSPELQMQYHIRQMALKLTANSMYGCLGFAHSRFFAQHLAALVTHKG 990
            ..|||.|:::|.|:.....:|..::|:.|:|.||||||||||||||:..|||:|:.||.|:|:||
pombe   896 ANLVERRRQIKGLLKDNSATPTQRLQWDIQQQALKLTANSMYGCLGYTKSRFYARPLAVLITYKG 960

  Fly   991 REILTNTQQLVQKMNYDVVYGDTDSLMINTNITDYDQVYKIGHNIKQSVNKLYKQLELDIDGVFG 1055
            ||.|.||::|..:|...|:||||||:|:|||:||.:...:||:..|:.||:.|.:||:|||.|:.
pombe   961 REALMNTKELADQMGLQVIYGDTDSVMLNTNVTDKNHALRIGNEFKEKVNERYSKLEIDIDNVYQ 1025

  Fly  1056 CLLLLKKKKYAAIKLSKDSKGNLRREQEHKGLDIVRRDWSQLAVMVGKAVLDEVLSEKPLEEKLD 1120
            .:||..||||||::|....|.||    :.||||:.||::..||....|..||::||.:..|..::
pombe  1026 RMLLHAKKKYAALQLDSQGKPNL----DVKGLDMKRREFCTLAKEASKFCLDQILSGELTETVIE 1086

  Fly  1121 AVHAQLEKIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALRMNRERNRRYKKGDMV 1185
            .:|:.|.....::..|..|...|:|..:|.:.|::|.|..::|.|||||: .:.|....:.||::
pombe  1087 NIHSYLMDFSEKMRNGKFPANKFIIFNRLGKNPEDYPNGKTMPFVQVALK-KKARGENVRVGDVI 1150

  Fly  1186 DYVICLDGTTNAAMQRAYHLDELKTSEDKKLQLDTNYYLGHQIHPVVTRMVEVLEGTDASRIAEC 1250
            .::|...........|||...|:..: :..|.:|.||||.|||.|.:.|::..:|||:.:|:|||
pombe  1151 PFIIAGSDADGHPADRAYSPQEIMNT-NSTLVIDYNYYLSHQILPPIERVIAPIEGTNRARLAEC 1214

  Fly  1251 LGMDPTKFRQNAQRTQRENTEQSEGESLLKTTL---QLYRLCEPFRFQCVTCKTEQLMASAYRPG 1312
            ||:|..|:..:       .|.:|......::||   |.:....|...:|.:|........:.:..
pombe  1215 LGLDARKYYSH-------ETSESSAFQRYESTLTDDQCFINVSPLLLKCPSCNATSFSLRSVKSL 1272

  Fly  1313 PSNSHIAVLQQCAKSECQTAPIQYLASVRNQLQLSMRQYVQRFYKNWLVCDHPDCNFNTRTHSLR 1377
            ....:...:      ||.........::..|....:|.::..:|:..||||...|...||..|:.
pombe  1273 KETLYANTV------ECDCGYEYSDFTIILQFSSQLRDFINLYYEGILVCDDSSCGNRTRQMSVY 1331

  Fly  1378 KKSHRPLCQKCRSGSLLRQYTERDLYNQLCYL 1409
            .|.   .|.|...||:..:|.:..||||:.:|
pombe  1332 GKR---CCNKSCRGSVHFEYNDEQLYNQIKFL 1360

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-alpha180	NP_536736.2	DNA_pol_alpha_N	24..83	CDD:289058	16/60 (27%)
		pol2	52..1237	CDD:273159	396/1242 (32%)
		DNA_polB_alpha_exo	567..789	CDD:99819	57/233 (24%)
		POLBc_alpha	852..1254	CDD:99915	180/409 (44%)
		zf-DNA_Pol	1286..1459	CDD:286132	27/124 (22%)
pol1	NP_594186.1	DNA_pol_alpha_N	15..78	CDD:289058	17/62 (27%)
		pol2	40..1200	CDD:273159	399/1246 (32%)
		DNA_polB_alpha_exo	522..757	CDD:99819	62/241 (26%)
		POLBc_alpha	829..1218	CDD:99915	173/394 (44%)
		zf-DNA_Pol	1238..1403	CDD:286132	30/132 (23%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	359	1.000	Domainoid score	I160
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H6802
Inparanoid	1	1.050	615	1.000	Inparanoid score	I152
OMA	1	1.010	-	-		QHG55031
OrthoFinder	1	1.000	-	-		FOG0004596
OrthoInspector	1	1.000	-	-		oto100924
orthoMCL	1	0.900	-	-		OOG6_102343
Panther	1	1.100	-	-	LDO	PTHR45861
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R343
SonicParanoid	1	1.000	-	-		X3211
TreeFam	1	0.960	-	-
	14	13.860