DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-alpha180 and Pole

DIOPT Version :9

Sequence 1:	NP_536736.2	Gene:	DNApol-alpha180 / 42553	FlyBaseID:	FBgn0259113	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_035262.2	Gene:	Pole / 18973	MGIID:	1196391	Length:	2283	Species:	Mus musculus

Alignment Length:	1648	Identity:	286/1648 - (17%)
Similarity:	493/1648 - (29%)	Gaps:	667/1648 - (40%)

- Green bases have known domain annotations that are detailed below.

  Fly   310 PKKETTGSPKESESEDISRLLNNWESICQMDDDFEKSVLTTEQDSTISSDQQLRFWYWEAYEDPV 374
            |..:..||..:..|..:|.|..           .|:|..|.:.|        |||.:     :.:
Mouse    13 PGADGEGSRDDGPSSSVSALKR-----------LERSQWTDKMD--------LRFGF-----ERL 53

  Fly   375 KMPGEVFLFGRTADGKSVCLRVQNINRVLYLLPRQFLLDPISKEPTKQKVTVADIY--------- 430
            |.|||     ||             ..::.:.|.:.|      :..|:.|:..|.|
Mouse    54 KEPGE-----RT-------------GWLINMHPTEIL------DEDKRLVSAVDYYFIQDDGSRF 94

  Fly   431 ---------------KEFDSEVANQLKLEFFRSRKVTK---------SFAHHAIGIEVPQSCDYL 471
                           |..|.||::.|..:|  ..|:.|         ...:|.:|::    ..|:
Mouse    95 KVALPYMPYFYIAARKGCDREVSSFLSKKF--QGKIAKLENVPKEDLDLPNHLVGLK----RSYI 153

  Fly   472 EVHYDGKKPLPNL-----SADKKYNSIAHIFGATTNALE-------------------------- 505
            ::.:...:.|..:     .|.||.....|.....|..|.
Mouse   154 KLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKKIADQLDNI 218

  Fly   506 ------------RFLLDRKIKGPCWLQVTGFKVSPTPMSWCNTEVTLTEPKNVELVQDKGKPAPP 558
                        |..:|.:|....|..|. |:.:..|:     |:|    :..:||:   :|.|
Mouse   219 VDMREYDVPYHIRLSIDLRIHVAHWYNVR-FRGNAFPV-----EIT----RRDDLVE---RPDP- 269

  Fly   559 PPLTLLSLNVRTS----MNPKTSRNEICMIS-------MLTHNRFHIDRP------APQPAFNRH 606
               .:|:.::.|:    ..|....::|.|||       .|..||..:...      .|:|.:...
Mouse   270 ---VVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGP 331

  Fly   607 MCALTRPAVVSWPLDLNFEMAKYKSTTVHKHDSERALLSWFLAQYQKIDADLIVTFD-------- 663
            .|....|                        |....:..|| ...|:....::||::
Mouse   332 FCVFNEP------------------------DEVHLIQRWF-EHIQETKPTIMVTYNGDFFDWPF 371

  Fly   664 ----------SMDCQLNVITDQIVALKIPQWSRMGRLRLSQSFGKRLLEHFVGRMVCDVKRSAEE 718
                      ||..::....|.....|.||...|..||.                   |||  :.
Mouse   372 VEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRW-------------------VKR--DS 415

  Fly   719 CIRARSYDLQTLCKQVLKLKESERMEVNADDLLEMYEKGESITKLISLTMQDNSYLLRLMCELNI 783
            .:...|::|:...|..|..   :.:|::.:|:..|..:........|::....:|.|.:.   .:
Mouse   416 YLPVGSHNLKAAAKAKLGY---DPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMK---YV 474

  Fly   784 MPLALQITNICGNTMTRTLQGGRSERNEFLLL-HAFHEKNYIVPDKK------------------ 829
            .|....:..|........|:.|.....|.||: .||| .|.|.|:|:
Mouse   475 HPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFH-ANIIFPNKQEQEFNKLTDDGHMLDAET 538

  Fly   830 ------------------PVSKRSGAGDTDATLSGADATM-----------------------QT 853
                              |...|......|..|...:.||                       |.
Mouse   539 YVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQI 603

  Fly   854 KKK----------------------AAYAGGLV---LEPMRGLYEKYVLLMDFN----------- 882
            |.|                      |.|...::   |:|...:.|......|||
Mouse   604 KTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAIVDEATCAACDFNKPGASCQRKMA 668

  Fly   883 -----SLYPSIIQEYNICFTTVQQPVDADELPTL-PD---------SKTEPGILPLQLKRL---- 928
                 ...|:...||:    .:|..:::::.|.| |:         |:.|..  ..:.:||
Mouse   669 WQWRGEFMPASRSEYH----RIQHQLESEKFPPLFPEGPARAFHELSREEQA--KYEKRRLADYC 727

  Fly   929 -----------VESRKEV-------------------------------KKLMAAPDLS------ 945
                       ||.|...                               |||.||.::.
Mouse   728 RKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVK 792

  Fly   946 --PELQMQYHIRQMALKLTANSMYGCLGFAHSRFFAQHLAALVTHKGREILTNTQQLVQKMNYDV 1008
              ..:::.|...|:|.|...||.||.:....:|:::..:|.:|...|..|:|..::|::::...:
Mouse   793 RCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPL 857

  Fly  1009 VYGDTDSL-------------------------------MIN-------TN-----ITDYDQVYK 1030
            .. |||.:                               |:|       ||     :|:...:..
Mouse   858 EL-DTDGIWCVLPNSFPENFVIKTTNAKKPKLTISYPGAMLNIMVKEGFTNHQYQELTEPSSLTY 921

  Fly  1031 IGHNIKQSVNKLYKQLELDIDGVFGCLLL--------LKKKKYAAIKLSKDSKGNLRREQEHKGL 1087
            :.|    |.|.::    .::||.:..::|        ..||:||..    :..|:|   .|.||.
Mouse   922 VTH----SENSIF----FEVDGPYLAMILPASKEEGKKLKKRYAVF----NEDGSL---AELKGF 971

  Fly  1088 DIVRRDWSQLAVMVGKAVLDEVLSEKPLEEKLDAVHAQLEKIKT-------QIAEGVVPLPLFVI 1145
            ::.||...||..:...:|.:..|....|||    |:..:.|:..       ..|..:....||.:
Mouse   972 EVKRRGELQLIKIFQSSVFEAFLKGSTLEE----VYGSVAKVADYWLDVLYSKAANMPDSELFEL 1032

  Fly  1146 ---TKQLTRTPQEYANSASLPHVQVALRMNRERNRRYKK--GDMVDYVICLDGTTNAAMQRAYHL 1205
               .:.::|..::|....| ..:..|.|:......:..|  |....|:|......:...:||..|
Mouse  1033 ISENRSMSRKLEDYGEQKS-TSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPL 1096

  Fly  1206 ---------------DELKTSE----DKKLQLDTNYY---LGHQIHPVVT--------------- 1233
                           ..||:|.    |.:..||.:||   ||..|..::|
Mouse  1097 AIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRV 1161

  Fly  1234 --------RMVE--------------VLEG------TDASRIA--------ECLGMDPTKFRQNA 1262
                    :::|              ||||      ..||..:        |.:|:.........
Mouse  1162 KHPDWLHKKLLEKNDIYKQKKISELFVLEGKRQIVMAQASENSLSLCTPDMEDIGLTKPHHSTVP 1226

  Fly  1263 QRTQRENTEQSEGES-----------------LLKTT-------LQLYRLCEPFRFQC---VTCK 1300
            ..|:|:...:::.||                 .|.||       ||.::  :.::.|.   :..:
Mouse  1227 VATKRKRVWETQKESQDIALTVPWQEVLGQPPSLGTTQEEWLVWLQFHK--KKWQLQAQQRLALR 1289

  Fly  1301 TEQLMASA--------YRPGPSNSHIAVLQQCAK------------SECQTAPIQYL-ASVRNQL 1344
            .:|.:.||        .|.|||....:.|::.|:            ||.:.|.:..| |.:.|.|
Mouse  1290 KKQRLESAEDMPRLGPIREGPSTGLGSFLRRTARSIMDLPWQIIQISETRQAGLFRLWAIIGNDL 1354

  Fly  1345 QLSMRQYVQRFYKNWLVCDHPDCNFNTRTHSLRKKSHRPLCQKCRSGSLLRQYTERDLYNQLCYL 1409
            ........:.||.|..|.                        |...|...|:.......:.:.|.
Mouse  1355 HCIKLSIPRVFYVNQRVA------------------------KAEDGPAYRKVNRALPRSNIVYN 1395

  Fly  1410 RFMFDLGKQTLQQ------KPTLTPELEQAYQ----LLYE---------TVDQQL-------QSS 1448
            .:.:.:.:...|:      .....|::|..|:    ||:.         .|::||       ::.
Mouse  1396 LYEYSVPEDMYQEHINEINTELSVPDIEGVYETQVPLLFRALVQLGCVCVVNKQLTRHLSGWEAE 1460

  Fly  1449 SYVIISLSKLFARSLAQMS-LQP 1470
            ::   :|..|..|||||.| |:|
Mouse  1461 TF---ALEHLEMRSLAQFSYLEP 1480

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-alpha180	NP_536736.2	DNA_pol_alpha_N	24..83	CDD:289058
		pol2	52..1237	CDD:273159	224/1310 (17%)
		DNA_polB_alpha_exo	567..789	CDD:99819	42/256 (16%)
		POLBc_alpha	852..1254	CDD:99915	115/642 (18%)
		zf-DNA_Pol	1286..1459	CDD:286132	35/222 (16%)
Pole	NP_035262.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..32		5/18 (28%)
		PolB	99..1165	CDD:223494	199/1168 (17%)
		Lyase_I_like	<125..>231	CDD:294018	12/109 (11%)
		DNA_polB_epsilon_exo	268..471	CDD:99822	43/255 (17%)
		POLBc_epsilon	531..1153	CDD:99918	112/648 (17%)
		DUF1744	1528..1924	CDD:285663
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1935..1968
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2014..2041
		CysB motif. /evidence=ECO:0000250\|UniProtKB:P15436	2218..2235
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900