DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-alpha180 and Pola1

DIOPT Version :9

Sequence 1:	NP_536736.2	Gene:	DNApol-alpha180 / 42553	FlyBaseID:	FBgn0259113	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_032918.1	Gene:	Pola1 / 18968	MGIID:	99660	Length:	1465	Species:	Mus musculus

Alignment Length:	1534	Identity:	601/1534 - (39%)
Similarity:	884/1534 - (57%)	Gaps:	168/1534 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 SESPSEPRAKRQRVDKNGRFAAMERLRQLK-GTKNKCKVEDQVDDVYDVVDEREYAKRAQEKYGD 65
            |.|.:..||:|::..|.||..|:|||::.| |.|.|.:||| :..||:.|||.:|:|..|.:..|
Mouse    19 SGSFAASRARREKKSKKGRQEALERLKKAKAGEKYKYEVED-LTSVYEEVDEEQYSKLVQARQDD 82

  Fly    66 DWI-EEDGTGYAEDLRDFFED--EDEY-------SDGE---EDRKDSKKKKGVAPNSKKRPRENE 117
            ||| ::||.||.||.|:.|:|  ||:.       |||:   :||||.||.....||
Mouse    83 DWIVDDDGIGYVEDGREIFDDDLEDDALDTCGKGSDGKAHRKDRKDVKKPSVTKPN--------- 138

  Fly   118 KPVTGKASIKNLF-SNAVPKKMDVKTSVKDDDILADILGEIKEEPAATSEKAEKVIAPAKISVTS 181
                   :||.:| ::|..|..|....:..||:|.|||.::..|.|.        |.|..:.:..
Mouse   139 -------NIKAMFIASAGKKTTDKAVDLSKDDLLGDILQDLNTETAQ--------ITPPPVLIPK 188

  Fly   182 RKFDAAAAKEYMNSFLNNIKVQEQERKKAEASSDNEMLERILKPKAAVPNTKVAFFSSPTIKKEP 246
            :|....|       .||...|.                    .|| |:|:.|.|   ||.::.||
Mouse   189 KKRSTGA-------LLNPFSVH--------------------TPK-AIPSGKPA---SPVLRNEP 222

  Fly   247 MPEKTPAKKA------TEDPFSDNEMDFSCLDDDENQFDVEKTQQTEKVSQTKTAAEKTSQ---- 301
            :....|.|:|      .:....::|.:...::.::..||  ::..||||.:....|:...|
Mouse   223 LLTPIPLKRAELAGELAQPECPEDEQELGVMEFEDGDFD--ESMDTEKVDEKPVTAKTWDQETEP 285

  Fly   302 -SKVAEKSAPKKETTGSPKESESEDISRLLNNWESICQMDDDFEKSVLTTEQDSTI------SSD 359
             .:|..::.|::.|| |..|:...|:|    .|:    :|.|.|......:.||:.      :.|
Mouse   286 VERVEHEADPERGTT-SYLENFLPDVS----CWD----IDQDDESIPQEVQVDSSNLPLVKGADD 341

  Fly   360 QQLRFWYW-EAYEDPVKMPGEVFLFGR-----TADGKSVCLRVQNINRVLYLLPRQFLLDPISKE 418
            :|:..:|| :|||||...||.|||||:     .....|.|:.|:||.|.||.|||:...|..:.:
Mouse   342 EQVFQFYWLDAYEDPYNQPGVVFLFGKVWIESVKTHVSCCVMVKNIERTLYFLPREMKFDLNTGK 406

  Fly   419 PTKQKVTVADIYKEFDSEVANQLKLEFFRSRKVTKSFAHHAIGIEVPQSCDYLEVHYDGKKPLPN 483
            .|...||:.|:|:||||:::.:.|:..|:|:.|.|::|...  .:||:..:||||.|..:  :|.
Mouse   407 ETAIPVTMKDVYEEFDSKISAKYKIMKFKSKIVEKNYAFEI--PDVPEKSEYLEVRYSAE--VPQ 467

  Fly   484 LSADKKYNSIAHIFGATTNALERFLLDRKIKGPCWLQVTGFKVSPTPMSWCNTEVTLTEPKNVEL 548
            |..:.|..:.:|:||..|::||.||::|||||||||:|...::...|:|||..||...:|..|.:
Mouse   468 LPQNLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKNPQLLNQPISWCKFEVMALKPDLVNV 532

  Fly   549 VQDKGKPAPPPPLTLLSLNVRTSMNPKTSRNEICMISMLTHNRFHIDRPAPQPAFNRHMCALTRP 613
            ::|    ..||||.::|.:::|..|.:..::||..::.|.|:.|.:|:..|:|.|..|.|.:::|
Mouse   533 IKD----VSPPPLVVMSFSMKTMQNVQNHQHEIIAMAALVHHSFALDKAPPEPPFQTHFCVVSKP 593

  Fly   614 AVVSWPLDLNFEMAKYKSTTVHKHDSERALLSWFLAQYQKIDADLIVTFDSMDCQLNVITDQIVA 678
            ....:|.|.. |:...|:..|....:||.|:.:|||:..|||.|::|..:....:|.|:..:|..
Mouse   594 KDCIFPCDFK-EVISKKNMKVEIAATERTLIGFFLAKVHKIDPDILVGHNICSFELEVLLQRINE 657

  Fly   679 LKIPQWSRMGRLRLS--------QSFGKRLLEHFVGRMVCDVKRSAEECIRARSYDLQTLCKQVL 735
            .|:|.||::||||.|        ..||:|  ....|||:|||:.||:|.|..:||.|..|.:|:|
Mouse   658 CKVPYWSKIGRLRRSNMPKLGSRSGFGER--NATCGRMICDVEISAKELIHCKSYHLSELVQQIL 720

  Fly   736 KLKESERMEVNADDLLEMYEKGESITKLISLTMQDNSYLLRLMCELNIMPLALQITNICGNTMTR 800
            |   :||:.:..:::..||.:...:..|:....:|..::|::|||||::|||||||||.||.|:|
Mouse   721 K---TERIVIPTENIRNMYSESSYLLYLLEHIWKDARFILQIMCELNVLPLALQITNIAGNIMSR 782

  Fly   801 TLQGGRSERNEFLLLHAFHEKNYIVPDK----KPVSKRSGAGDTDATLSG-ADATMQTKKKAAYA 860
            ||.|||||||||||||||:|.|||||||    ||..|   .||.|..:.| .:...:.:|||.||
Mouse   783 TLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQK---LGDEDEEIDGDTNKYKKGRKKATYA 844

  Fly   861 GGLVLEPMRGLYEKYVLLMDFNSLYPSIIQEYNICFTTVQ----------QPVDADELPTLPDSK 915
            |||||:|..|.|:|::||:||||||||||||:||||||||          :..:.:::|.|||..
Mouse   845 GGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVTSEVQKATEDEEQEQIPELPDPN 909

  Fly   916 TEPGILPLQLKRLVESRKEVKKLMAAPDLSPELQMQYHIRQMALKLTANSMYGCLGFAHSRFFAQ 980
            .|.||||.::::|||.||:||:||...||:|:|.:||.|||.|||||||||||||||::|||:|:
Mouse   910 LEMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAK 974

  Fly   981 HLAALVTHKGREILTNTQQLVQKMNYDVVYGDTDSLMINTNITDYDQVYKIGHNIKQSVNKLYKQ 1045
            .||||||:||||||.:|:.:|||||.:|:||||||:|||||.|:.::|:|:|:.:|..||||||.
Mouse   975 PLAALVTYKGREILMHTKDMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKL 1039

  Fly  1046 LELDIDGVFGCLLLLKKKKYAAIKLSKDSKGNLRREQEHKGLDIVRRDWSQLAVMVGKAVLDEVL 1110
            ||:|||.||..|||||||||||:.:...|.||...:||.||||||||||..||...|..|:.::|
Mouse  1040 LEIDIDAVFKSLLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQIL 1104

  Fly  1111 SEKPLEEKLDAVHAQLEKIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALRMNRER 1175
            |::..:..::.:..:|.:|...:..|.||:..|.|.|.||:.||:|.:..|||||.|||.:|.:.
Mouse  1105 SDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVHVALWINSQG 1169

  Fly  1176 NRRYKKGDMVDYVICLDGTTNAAMQRAYHLDELKTSEDKKLQLDTNYYLGHQIHPVVTRMVEVLE 1240
            .|:.|.||.|.||||.||:...|.||||..::|:..::  |.:||.|||..||||||.|:.|.::
Mouse  1170 GRKVKAGDTVSYVICQDGSNLTATQRAYAPEQLQKLDN--LAIDTQYYLAQQIHPVVARICEPID 1232

  Fly  1241 GTDASRIAECLGMDPTKFRQNAQRTQRENTEQSEGESLLKTTLQLYRLCEPFRFQCVTCKTEQLM 1305
            |.||..||..||:|.|:||.:......||.....|.:.| |..:.|:.||.|:..|.:|.||.:.
Mouse  1233 GIDAVLIALWLGLDSTQFRVHQYHKDEENDALLGGPAQL-TDEEKYKDCEKFKCLCPSCGTENIY 1296

  Fly  1306 ASAYRPG-----PSNSHIAVLQQCAKSECQTAPIQYLASVRNQLQLSMRQYVQRFYKNWLVCDHP 1365
            .:.:...     ||      |.:|:..:|:.:|:.::..:.|:|.:.:|:.::::|..||:|:.|
Mouse  1297 DNVFEGSGLDMEPS------LYRCSNVDCKVSPLTFMVQLSNKLIMDIRRCIKKYYDGWLICEEP 1355

  Fly  1366 DCNFNTRTHSLRKKSHRPLCQKCRSGSLLRQYTERDLYNQLCYLRFMFD----LGKQTLQQKPTL 1426
            .|....|...|....:.|||..|....|..:|:::.||.|||:.|::||    |.|.|..:|..|
Mouse  1356 TCCSRLRRLPLHFSRNGPLCPVCMKAVLRPEYSDKSLYTQLCFYRYIFDADCALEKLTEHEKDKL 1420

  Fly  1427 TPE-----LEQAYQLLYETVDQQLQSSSYVIISLSKLFA 1460
            ..:     :.|.|:.:....:|.|..|.|..::||||||
Mouse  1421 KKQFFPLRVLQDYRKVKNIAEQFLSWSGYSEVNLSKLFA 1459

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-alpha180	NP_536736.2	DNA_pol_alpha_N	24..83	CDD:289058	29/60 (48%)
		pol2	52..1237	CDD:273159	505/1244 (41%)
		DNA_polB_alpha_exo	567..789	CDD:99819	77/229 (34%)
		POLBc_alpha	852..1254	CDD:99915	222/411 (54%)
		zf-DNA_Pol	1286..1459	CDD:286132	53/186 (28%)
Pola1	NP_032918.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	20..39		7/18 (39%)
		DNA_pol_alpha_N	41..101	CDD:289058	29/60 (48%)
		pol2	56..1229	CDD:273159	512/1258 (41%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	105..135		11/29 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	261..297		9/37 (24%)
		DNA_polB_alpha_exo	539..771	CDD:99819	81/237 (34%)
		DNA-binding. /evidence=ECO:0000255	654..719		27/66 (41%)
		POLBc_alpha	836..1246	CDD:99915	222/411 (54%)
		DNA-binding. /evidence=ECO:0000255	1249..1380		36/137 (26%)
		zf-DNA_Pol	1269..1458	CDD:286132	55/195 (28%)
		CysB motif	1352..1378		8/25 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850541
Domainoid	1	1.000	476	1.000	Domainoid score	I434
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H6802
Inparanoid	1	1.050	994	1.000	Inparanoid score	I303
Isobase	1	0.950	-	0	Normalized mean entropy	S1805
OMA	1	1.010	-	-		QHG55031
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0004596
OrthoInspector	1	1.000	-	-		oto92888
orthoMCL	1	0.900	-	-		OOG6_102343
Panther	1	1.100	-	-	LDO	PTHR45861
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R343
SonicParanoid	1	1.000	-	-		X3211
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.740