DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment E2f1 and E2f1

DIOPT Version :9

Sequence 1:	NP_001262809.1	Gene:	E2f1 / 42550	FlyBaseID:	FBgn0011766	Length:	821	Species:	Drosophila melanogaster
Sequence 2:	NP_001094248.1	Gene:	E2f1 / 399489	RGDID:	728892	Length:	432	Species:	Rattus norvegicus

Alignment Length:	399	Identity:	122/399 - (30%)
Similarity:	175/399 - (43%)	Gaps:	97/399 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly   221 VAKPASHHPFSLSTPQQQLAASVA-----------SSSSSGDRNRADTSLGILTKKFVDLLQESP 274
            :.:|.......|.|..|.||.|..           ...|.|:::|.:|||.:.||:|::||..|.
  Rat    81 LGRPPVKRRLDLETDHQYLAGSSGPFRGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSA 145

  Fly   275 DGVVDLNEASNRLHVQKRRIYDITNVLEGINILEKKSKNNIQWRCGQSMVS-QERSRHIEADSLR 338
            |||||||.|:..|.||||||||||||||||.::.|||||:|||...::||. .:|...:..|..:
  Rat   146 DGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSRTMVGIGQRLEGLTQDLQQ 210

  Fly   339 LEQQENELNKAIDLMRENLAEISQEVENSGGMAYVTQNDLLNVDLFKDQIVIVIKAPPEAKLVRS 403
            |::.|.:|:..:.:....|..:|:: .:...:||||..||.::....:|:|||||||||.:|...
  Rat   211 LQESEQQLDHLMHICTTQLQLLSED-SDIQRLAYVTCQDLRSIADPAEQMVIVIKAPPETQLQAV 274

  Fly   404 ATALKFQPLPFNLNLPNTKLPREIYVKAENSGEINVFLCHDTSPENSPIAPG-AGYVGAPGAGCV 467
            .:|..||                |.:|:: .|.|:||||.:.|.|.  |:|| ..|....|..
  Rat   275 DSAETFQ----------------ISLKSK-QGPIDVFLCPEESAEG--ISPGRTSYQETSGED-- 318

  Fly   468 RTATSTRLHPLTNQRLNDPLFNNIDAMSTKGLFQTPYRSARNLSKSIEEAAKQSQPEYNNICDIA 532
            |.|.|....|..:.....|..:             |.:|...|.   :||.   .|...|
  Rat   319 RNADSGTAGPPPSPPSTSPTLD-------------PSQSLLGLE---QEAV---LPRIGN----- 359

  Fly   533 MGQHHNLNQQQQQQQQQLLQQPEEDDVDVELNQLVPTLTNPVVRTHQFQQHQQPSIQELFSSLTE 597
                              |:.|.|:|           ..:|:|......:|    ::|.||.|..
  Rat   360 ------------------LRAPMEED-----------RLSPLVAADSLLEH----VKEDFSGLLP 391

  Fly   598 S-----SPP 601
            .     |||
  Rat   392 GEFISLSPP 400

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
E2f1	NP_001262809.1	E2F_TDP	256..318	CDD:280479	41/61 (67%)
		E2F_DD	327..444	CDD:304549	35/116 (30%)
		coiled coil	327..362	CDD:271137	6/34 (18%)
E2f1	NP_001094248.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	39..85		0/3 (0%)
		Cyclin A:CDK2 binding. /evidence=ECO:0000250\|UniProtKB:Q01094	65..106		7/35 (20%)
		Interaction with BIRC2/c-IAP1. /evidence=ECO:0000250\|UniProtKB:Q01094	87..189		53/113 (47%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	98..126		11/38 (29%)
		E2F_TDP	127..190	CDD:396755	39/63 (62%)
		Leucine-zipper. /evidence=ECO:0000250\|UniProtKB:Q01094	151..172		16/20 (80%)
		DEF box. /evidence=ECO:0000250\|UniProtKB:Q01094	156..192		20/36 (56%)
		Required for interaction with TRIM28. /evidence=ECO:0000250\|UniProtKB:Q01094	190..377		49/187 (26%)
		Dimerization. /evidence=ECO:0000255	193..282		26/88 (30%)
		E2F_DD	219..299	CDD:271137	25/79 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	297..342		16/45 (36%)
		Transactivation. /evidence=ECO:0000250\|UniProtKB:Q01094	363..432		11/68 (16%)
		RB1 binding. /evidence=ECO:0000250\|UniProtKB:Q01094	404..421		4/16 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	59	1.000	Domainoid score	I10380
eggNOG	1	0.900	-	-		E1_KOG2577
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	170	1.000	Inparanoid score	I4037
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000356
OrthoInspector	1	1.000	-	-		otm46224
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR12081
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X977
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	11	10.920