DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment E2f1 and E2F4

DIOPT Version :9

Sequence 1:	NP_001262809.1	Gene:	E2f1 / 42550	FlyBaseID:	FBgn0011766	Length:	821	Species:	Drosophila melanogaster
Sequence 2:	NP_001941.2	Gene:	E2F4 / 1874	HGNCID:	3118	Length:	413	Species:	Homo sapiens

Alignment Length:	574	Identity:	142/574 - (24%)
Similarity:	237/574 - (41%)	Gaps:	189/574 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   250 GDRNRADTSLGILTKKFVDLLQESPDGVVDLNEASNRLHV-QKRRIYDITNVLEGINILEKKSKN 313
            |..:|.:.|||:||.|||.||||:.|||:||..|::.|.| |||||||||||||||.::||||||
Human    13 GTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKN 77

  Fly   314 NIQWR-----CGQSMVSQERSRHIEADSLRLEQQENELNKAIDLMRENLAEISQEVENSGGMAYV 373
            :|||:     |....:: ::...::|:...|:|:|.||::....:::::..::::|:|| .:|||
Human    78 SIQWKGVGPGCNTREIA-DKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNS-CLAYV 140

  Fly   374 TQNDLLNVDLFKDQIVIVIKAPPEAKLVRSATALKFQPLPFNLNLPNTKLPREIYVKAENSGEIN 438
            |..|:..  .|....::.|:||       |.|:|:. |:|..|   |.:...:|::|:. ||.|.
Human   141 THEDICR--CFAGDTLLAIRAP-------SGTSLEV-PIPEGL---NGQKKYQIHLKSV-SGPIE 191

  Fly   439 VFLCHDTSPENSPIAPGAGYVGAPGAGCVRTATSTRLHPLTNQRLNDPLFNNIDAMSTKGLFQTP 503
            |.|.:..:..:.|:|     |..|..                    :.|..:..|:||......|
Human   192 VLLVNKEAWSSPPVA-----VPVPPP--------------------EDLLQSPSAVSTPPPLPKP 231

  Fly   504 YRSARNLSKSIEEAAKQSQPEYNNICDIAMGQHHNLNQQQQQQQQQLLQQPEEDDVDVELNQLVP 568
                 .|::| :||::.:.|                                         ||.|
Human   232 -----ALAQS-QEASRPNSP-----------------------------------------QLTP 249

  Fly   569 TLTNPVVRTHQFQQHQQPSIQELFSSLTESSPPTPTKRRREAAAAAIAAGSSTTATTTLNSHNNR 633
            |   .|..:.:.|....|:     :.:|.|..| .|..:.....:::..|.:|..|..|.|
Human   250 T---AVPGSAEVQGMAGPA-----AEITVSGGP-GTDSKDSGELSSLPLGPTTLDTRPLQS---- 301

  Fly   634 NHSNHSNHSNHSSSNNSKSQPPTIGYGSSQRRSDVPMYNCAMEGATTTSATADTTAATSRSAAAS 698
                                                            ||..|:::::|.|:::|
Human   302 ------------------------------------------------SALLDSSSSSSSSSSSS 318

  Fly   699 SLQMQFAAVAESNNGSSSGGGGGGGGYGSIAGAGANADPHQPYSHDRNSLPPGVADCDANSNSSS 763
            |         .||:.||||..              .:...:|...|    |.||.:..  ...|.
Human   319 S---------NSNSSSSSGPN--------------PSTSFEPIKAD----PTGVLELP--KELSE 354

  Fly   764 VTLQGLDALFNDIGSDYFSNDIAFVSI----NPPDDNDYPYALNANEGIDRLFD 813
            :.....:.:.:::..:..|::: |..:    .||.|:||.|.|:.:||:..|||
Human   355 IFDPTRECMSSELLEELMSSEV-FAPLLRLSPPPGDHDYIYNLDESEGVCDLFD 407

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
E2f1	NP_001262809.1	E2F_TDP	256..318	CDD:280479	43/62 (69%)
		E2F_DD	327..444	CDD:304549	32/116 (28%)
		coiled coil	327..362	CDD:271137	6/34 (18%)
E2F4	NP_001941.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..20		2/6 (33%)
		E2F_TDP	18..83	CDD:367033	44/64 (69%)
		Leucine-zipper	43..65		14/21 (67%)
		DEF box	48..85		26/36 (72%)
		Dimerization. /evidence=ECO:0000255	86..181		26/109 (24%)
		E2F_DD	95..198	CDD:271137	32/117 (27%)
		coiled coil	95..130	CDD:271137	6/34 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	211..340		40/279 (14%)
		Transactivation. /evidence=ECO:0000255	337..413		20/78 (26%)
		HCFC1-binding-motif (HBM)	389..392		1/2 (50%)
		Interaction with RBL1 and RBL2. /evidence=ECO:0000255	390..407		7/16 (44%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG2577
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	156	1.000	Inparanoid score	I4302
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000356
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101186
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3254
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	6	5.790