DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment E2f1 and E2F1

DIOPT Version :9

Sequence 1:	NP_001262809.1	Gene:	E2f1 / 42550	FlyBaseID:	FBgn0011766	Length:	821	Species:	Drosophila melanogaster
Sequence 2:	NP_005216.1	Gene:	E2F1 / 1869	HGNCID:	3113	Length:	437	Species:	Homo sapiens

Alignment Length:	403	Identity:	113/403 - (28%)
Similarity:	177/403 - (43%)	Gaps:	102/403 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly   221 VAKPASHHPFSLSTPQQQLAASVASS-----------SSSGDRNRADTSLGILTKKFVDLLQESP 274
            :.:|.......|.|..|.||.|...:           .|.|:::|.:|||.:.||:|::||..|.
Human    83 LGRPPVKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSA 147

  Fly   275 DGVVDLNEASNRLHVQKRRIYDITNVLEGINILEKKSKNNIQWRCGQSMVS-QERSRHIEADSLR 338
            |||||||.|:..|.||||||||||||||||.::.|||||:|||....:.|. ..|...:..|..:
Human   148 DGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQ 212

  Fly   339 LEQQENELNKAIDLMRENLAEISQEVENSGGMAYVTQNDLLNVDLFKDQIVIVIKAPPEAKLVRS 403
            |::.|.:|:..:::....|..:|::.: |..:||||..||.::....:|:|:|||||||.:|...
Human   213 LQESEQQLDHLMNICTTQLRLLSEDTD-SQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAV 276

  Fly   404 ATALKFQPLPFNLNLPNTKLPREIYVKAENSGEINVFLCHDTSPENS--PIAPGAGYVGAPGAGC 466
            .::..||                |.:|:: .|.|:||||    ||.:  .|:||
Human   277 DSSENFQ----------------ISLKSK-QGPIDVFLC----PEETVGGISPG----------- 309

  Fly   467 VRTATSTRLHPLTNQRLNDPLFNNIDAMSTKGLFQTPYRSARNLSKSIEEAAKQSQPEYNNICDI 531
                 .|....:|::..|                           ::.:.|...|.|..:....:
Human   310 -----KTPSQEVTSEEEN---------------------------RATDSATIVSPPPSSPPSSL 342

  Fly   532 AMGQHHNLNQQQQQ---QQQQLLQQPEEDDVDVELNQLVPTLTNPVVRTHQFQQHQQPSIQELFS 593
            ......:|...:|:   .:...|:.|.::|           ..:|:|......:|    ::|.||
Human   343 TTDPSQSLLSLEQEPLLSRMGSLRAPVDED-----------RLSPLVAADSLLEH----VREDFS 392

  Fly   594 SLTES-----SPP 601
            .|...     |||
Human   393 GLLPEEFISLSPP 405

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
E2f1	NP_001262809.1	E2F_TDP	256..318	CDD:280479	41/61 (67%)
		E2F_DD	327..444	CDD:304549	34/116 (29%)
		coiled coil	327..362	CDD:271137	6/34 (18%)
E2F1	NP_005216.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	42..87		0/3 (0%)
		Cyclin A:CDK2 binding. /evidence=ECO:0000269\|PubMed:8033208	67..108		7/24 (29%)
		Interaction with BIRC2/c-IAP1. /evidence=ECO:0000269\|PubMed:21653699	89..191		50/101 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	101..128		5/26 (19%)
		E2F_TDP	129..192	CDD:280479	42/62 (68%)
		Leucine-zipper	153..174		15/20 (75%)
		DEF box	158..194		25/35 (71%)
		Required for interaction with TRIM28. /evidence=ECO:0000269\|PubMed:17704056	192..382		53/265 (20%)
		Dimerization. /evidence=ECO:0000255	195..284		27/105 (26%)
		E2F_DD	202..301	CDD:271137	35/120 (29%)
		coiled coil	202..236	CDD:271137	6/33 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	300..349		10/91 (11%)
		Transactivation	368..437		12/53 (23%)
		RB1 binding. /evidence=ECO:0000269\|PubMed:12598654	409..426
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	59	1.000	Domainoid score	I10661
eggNOG	1	0.900	-	-		E1_KOG2577
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000356
OrthoInspector	1	1.000	-	-		otm42079
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR12081
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3254
SonicParanoid	1	1.000	-	-		X977
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	10	9.900