DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment e and sib1

DIOPT Version :9

Sequence 1:	NP_524431.2	Gene:	e / 42521	FlyBaseID:	FBgn0000527	Length:	879	Species:	Drosophila melanogaster
Sequence 2:	NP_593102.1	Gene:	sib1 / 2541451	PomBaseID:	SPAC23G3.02c	Length:	4924	Species:	Schizosaccharomyces pombe

Alignment Length:	707	Identity:	186/707 - (26%)
Similarity:	308/707 - (43%)	Gaps:	126/707 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    22 LHRIFEEQQLRH--ADKVALIYQPSTTGQGMAPS--QSSYRQMNERANRAARLLVAETHGRFLQP 82
            ||..|....|.|  .|.:..||:   .|..:..:  ..|:.:::..|.:.::|:..:..
pombe   210 LHGCFYHNTLSHPYQDALKFIYE---IGDDLEDTFRSFSFLELHSLAIKLSKLVTCKNE------ 265

  Fly    83 NSDGDFIVAVCMQPSEGLVTTLLAIWKAGGAYLPIDPSFPANRIHHILLEAKPTLVIRDDDIDAG 147
                  :|.:.:..|..|...:|||..:|.||.|||......|:|.|..:...:..|..::. ..
pombe   266 ------VVPIMVSHSPALFVGILAILYSGNAYCPIDVETRTERVHFISKDVDASFAIVSEEF-VN 323

  Fly   148 RFQGTPTLSTTELYAKSLQLAGSNLLSEEMLRGGNDHIAIVLYTSGSTGVPKGVRLPHESILNRL 212
            ||.....:.....|.:|:::...:.:.........|.:|.|||||||||.||||.:.|.:..|.:
pombe   324 RFPNNTIILKVPEYNESMEIKVDDEIPPFPFPESLDSVAYVLYTSGSTGNPKGVAISHRAATNSI 388

  Fly   213 Q---WQWATFPYTANEAVSVFKTALTFVDSIAELWGPLMCGLAILVVPKAVTKDPQRLVALLE-- 272
            :   :.:..|.....:|...|.. :||..|:.|::|....||.:      ||...|.|:..||
pombe   389 KSHGYLYPMFGLERGDAWLQFAN-VTFDVSVFEIFGNWNNGLTL------VTSKRQNLIGNLEYL 446

  Fly   273 --RYKIRRLVLVPTLLRSLLMYLKMEGGGAAQKLLYNLQIWVCSGEPLSVSLASSFFDYFDEGVH 335
              .|||..|.|.||:...:.:       ...::|..::::.:..||    .|.:...|::.|
pombe   447 IYDYKIAALELTPTVANVISL-------DENKELFTSVRMLITIGE----LLTNRIIDFWGE--- 497

  Fly   336 RLYNFYGSTEVLGDVTYFACESKKQLSLYDNVPIGIPLSNT---VVYLLDADYRP--VKNGEIGE 395
            ||.|.||.||....||  ...||...::|   .:|:||.:.   ||.|...|.:|  :..|.:||
pombe   498 RLVNAYGPTEAAIHVT--LNPSKALTTVY---LVGVPLQSATICVVSLPTEDSQPHVLHEGFLGE 557

  Fly   396 IFASGLNLAAGYVNGRDPERFLENPLAVEKKY----ARLYRTGDYGSLKNGSIMYEGRT--DSQV 454
            :..:|..|::||:|  .||  :.....||.:|    ..:|||||.|.:.||.:...||.  |.|:
pombe   558 VCIAGPQLSSGYIN--RPE--INAKAFVEVQYNEQTLSIYRTGDLGRIINGKLYIFGRIAGDMQI 618

  Fly   455 KIRGHRVDLSEVEKNVAELPLVDKAIVLCYHAGQVDQAILAFVKLRDDAPMVT-----EMQMEAR 514
            ||||.|:::.|:|..:|  |.||.               ||..|:.|:  :|.     ||::...
pombe   619 KIRGRRIEIGEIESKLA--PSVDS---------------LAIEKIGDN--LVAFYVGDEMKLRKH 664

  Fly   515 LKDKLADYMTP-QVVILEHVPLLVNGKVDRQALLKTYETANNNEGDSSIVLDFDYSQVPEDLKLT 578
            .:..||.:|.| :.|.|...|.|.:||.||:||...:..::.:..|.|   .|:.|::     |.
pombe   665 AESHLASWMCPTKYVGLPGFPRLASGKTDRKALKIQFSASDKHSTDFS---SFNQSEL-----LV 721

  Fly   579 ARDLFE----TVGGVIGRSTRATLAPHSNFYELGGNSLNSIFTVTLLREKGYNIGISEFIAAKNL 639
            |..|.|    ....|:      :::..|:.::||.:||:.::.|..:|..|:....|..:.:|.:
pombe   722 ANVLHEICEKRFNSVV------SISRFSSIFDLGLDSLDIVYFVRKIRSLGFEANPSIVLTSKVV 780

  Fly   640 GEIIEKM-AANHDAVQLEEESLNACPHLKMEAVPLRLEHRQEVIDIIVASFYNKADL 695
            .:|.|.: ....:.||.:...||.|..|:.              .::..||.|..:|
pombe   781 FKIAESLILLKPNEVQSKNNFLNKCTPLQK--------------GMLYESFSNNGNL 823

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
e	NP_524431.2	A_NRPS	35..546	CDD:213296	148/536 (28%)
		AA-adenyl-dom	56..481	CDD:273779	125/442 (28%)
		PP-binding	580..644	CDD:278949	14/67 (21%)
sib1	NP_593102.1	AFD_class_I	217..698	CDD:302604	151/545 (28%)
		AA-adenyl-dom	265..640	CDD:273779	121/419 (29%)
		Condensation	806..1077	CDD:305010	5/32 (16%)
		EntF	1013..>1152	CDD:223951
		PP-binding	1224..1291	CDD:278949
		Condensation	1326..1599	CDD:305010
		EntF	1535..2125	CDD:223951
		AFD_class_I	1760..>2122	CDD:302604
		PP-binding	2176..2242	CDD:278949
		Condensation	2310..2568	CDD:305010
		A_NRPS_SidN3_like	2726..3226	CDD:213285
		AA-adenyl-dom	2753..3139	CDD:273779
		PP-binding	3259..3320	CDD:278949
		Condensation	3361..3613	CDD:305010
		EntF	3549..>3698	CDD:223951
		Condensation	3866..4150	CDD:305010
		EntF	4087..>4263	CDD:223951
		PP-binding	4411..4474	CDD:278949
		Condensation	4508..4789	CDD:305010
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	110	1.000	Domainoid score	I1606
eggNOG	1	0.900	-	-		E1_COG1020
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002418
OrthoInspector	1	1.000	-	-		oto100994
orthoMCL	1	0.900	-	-		OOG6_100454
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5888
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	7	6.740