DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment e and nrps-1

DIOPT Version :9

Sequence 1:	NP_524431.2	Gene:	e / 42521	FlyBaseID:	FBgn0000527	Length:	879	Species:	Drosophila melanogaster
Sequence 2:	NP_499488.3	Gene:	nrps-1 / 190498	WormBaseID:	WBGene00013440	Length:	2942	Species:	Caenorhabditis elegans

Alignment Length:	709	Identity:	160/709 - (22%)
Similarity:	276/709 - (38%)	Gaps:	209/709 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 SLPQLSIV-----KGLQQDFVPRALHRIFE--------------EQQLRHADKVALIYQPSTTGQ 48
            |:|::.::     ||.......|...:||.              .||...|:..::.   :||.:
Worm  2161 SVPEMQVILNIVLKGFNDTPCRRKHEKIFYGPRKDTPNLSIPSIYQQFFTANSTSIF---ATTSK 2222

  Fly    49 GMAPSQSSYRQMNERANRAARLLVAETHGRFL----QPNSDGDFIVAVCMQPSEGLVTTL--LAI 107
            .......:||::::.::..::        :||    ||....|.|.....:.:|   ||:  ||:
Worm  2223 TSTTYAQAYRRLSQYSSCLSK--------KFLTSKAQPIRSDDVISVAGTRSTE---TTMKCLAV 2276

  Fly   108 WKAGGAYLPIDPSFPANRIHHILLEAKPTLVIRDDDID---AGRFQGTPTLSTTELYAKSLQLAG 169
            ...|.||||||.|:|..|        |.| :::|...:   .||....|.
Worm  2277 QFTGAAYLPIDASYPEER--------KKT-ILKDSVFNFEYNGRVDQEPR--------------- 2317

  Fly   170 SNLLSEEMLRGGNDHIAI--------VLYTSGSTGVPKGVRLPHESILNRLQWQWATFPYTANEA 226
                        :.|.||        ::.|||:||.||.|.:..:|:||.......|...:::..
Worm  2318 ------------HRHFAISTDYCLSYIITTSGTTGTPKSVAIGAKSLLNLFLSSTLTMKCSSSSR 2370

  Fly   227 VSVFKTALTFVDSIAE-----------LWGPLMCGLAILVVPKAVTKDPQRLVALLERYKIRRLV 280
            ...| |...|.:|:.|           ::|||..             ||......:|...|...:
Worm  2371 TYQF-TNFVFDNSVLEVSMSIASQGTLVYGPLCF-------------DPADFEKSIETEGITHCL 2421

  Fly   281 LVPTLLRSLLMYLKMEGGGAAQKLLYNLQIWVCSGEPLSVSLASSFFDYFDEGVHRLYNFYGSTE 345
            |.|:|::|..:           ..:.||..||..||||..||..|....   |:..:.| ||.||
Worm  2422 LFPSLVQSFNI-----------SKIKNLAYWVVGGEPLPQSLLDSALAL---GIKVIQN-YGPTE 2471

  Fly   346 VLGDVTYFACESKKQLSLYD-NVPIGIPLSNTVVYLLDADYRPVKNGEIGEIFASGLNLAAGYVN 409
                .|.||.  .|.:...| ...||:|..|:.:       |.|:..:.||:..|||.:..||:|
Worm  2472 ----TTAFAI--AKHMKKGDAGCQIGLPAVNSKI-------RIVEGEDQGELLISGLGIMRGYLN 2523

  Fly   410 GRDPERFLENPLAVEKKYARLYRTGDYGSLKNGSIMYEGRTDSQVKIRGHRVDLSEVEKNVAE-- 472
                           :|.::.|.:||...::.|.:.:.||||:|:||||:||:|:|:||.:..
Worm  2524 ---------------RKESQWYASGDVCRIEKGVVEFVGRTDTQIKIRGYRVELTEIEKTIESRN 2573

  Fly   473 -----LPLVDKAI--VLCYHAGQVDQAILAFVKL-RDDAPMVTEMQMEARLKDKLADYMTPQVVI 529
                 ..|.||..  :..::.|:::       || .:||            ::....|:.|..:.
Worm  2574 GIASCKALFDKESQQIHVFYTGELE-------KLSSNDA------------REHCERYLEPHKIP 2619

  Fly   530 -----LEHVPLLVNGKVDRQALLKTYETANNNEGDSSIVLDFDYSQVPEDLKLTARDLFETVGGV 589
                 ::..||..|.|:|...|::             :|.:..:..:|..|:.....::.::...
Worm  2620 STFNGIDKFPLTGNSKIDTLKLME-------------LVKNLPHPTLPNSLESDLSSIWTSLLNC 2671

  Fly   590 IGRSTRATLAPHSNFYELGGNSLNSIFTVTLLREK-GYNIGISEFIAAKNLGEIIEKMA 647
            ...|      |..:|:.:||:||..:....|:..| |.::.:.|.:......:::|.:|
Worm  2672 PEPS------PSDHFFLIGGHSLLLVRLRHLIETKLGISLKVPEILQNLKFSDMLELLA 2724

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
e	NP_524431.2	A_NRPS	35..546	CDD:213296	133/554 (24%)
		AA-adenyl-dom	56..481	CDD:273779	119/462 (26%)
		PP-binding	580..644	CDD:278949	11/64 (17%)
nrps-1	NP_499488.3	Condensation	441..686	CDD:305010
		HxxPF_rpt	712..802	CDD:290463
		AFD_class_I	860..1269	CDD:302604
		AA-adenyl-dom	860..1207	CDD:273779
		PP-binding	1312..1361	CDD:278949
		Condensation	1379..1606	CDD:305010
		PP-binding	1712..1776	CDD:278949
		Condensation	1800..>1941	CDD:305010
		AFD_class_I	2223..2641	CDD:302604	131/540 (24%)
		AA-adenyl-dom	2253..2578	CDD:273779	110/420 (26%)
		PP-binding	2661..2723	CDD:278949	12/67 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	75	1.000	Domainoid score	I5932
eggNOG	1	0.900	-	-		E1_COG1020
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S6452
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002418
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100454
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5888
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	7	6.690