DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Usp8 and DOA4

DIOPT Version :9

Sequence 1:	NP_650948.2	Gene:	Usp8 / 42509	FlyBaseID:	FBgn0038862	Length:	896	Species:	Drosophila melanogaster
Sequence 2:	NP_010354.3	Gene:	DOA4 / 851641	SGDID:	S000002476	Length:	926	Species:	Saccharomyces cerevisiae

Alignment Length:	1009	Identity:	235/1009 - (23%)
Similarity:	394/1009 - (39%)	Gaps:	255/1009 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 KLKKLHMSSNLDDLEKMSIIPDLRSKGMKILLNTARKLYMTAEEYRLDGDEELAYITYMKYFNML 67
            |.|...:||.||....:..|....||.:|.:::...|..|.:....:  ..:|.|: |.|..:::
Yeast    42 KAKDQDLSSLLDKCIDILSIYKKNSKDIKNIISCKNKGAMISSNSVM--IIQLNYV-YYKVIHII 103

  Fly    68 --TAIHKKSDYPS---HKTTVRQMLGDTESN--------RRIMDTL---------EEIINSLRHR 110
              |.|...|::..   ||:|..:..|:..:|        ..:::||         :..|.|...:
Yeast   104 VTTNIPHLSEFAKIKLHKSTSDEGNGNNNNNEFQLMNIYNTLLETLLKDENIAKIKSFIKSSIKQ 168

  Fly   111 YAQQHQAPEPIAPDLVSNGRAGVDSPITQPTQYARLGLITCQDLYRRMQEKSVLVMDCRPSADYE 175
            ....|:..|   .:|:..|     |.||.    .:|..:..........:..:|::|.|...::.
Yeast   169 TKLNHEQEE---CNLMRTG-----SYITS----NQLNSLISSSANSASSQMEILLIDIRSRLEFN 221

  Fly   176 ASHL---TYYC----AFNV--------PEELITPGMSAGRLQARLSSSAKASWASRSVKDSVVLM 225
            .||:   ...|    :|.:        .:.|||...|..::           :.||::...::|.
Yeast   222 KSHIDTKNIICLEPISFKMSYSDHDLEKKSLITSPNSEIKM-----------FQSRNLFKFIILY 275

  Fly   226 ----DWNTKDAQPATNTAISTLLDILKNWDPDVTYRAPIQ-------IVEGGYEYFIMM-YPTHC 278
                ::|.|..        |.|||||.|.    ::..||.       |:|.|:..::.. |
Yeast   276 TDANEYNVKQQ--------SVLLDILVNH----SFEKPISDDFTKIFILESGFPGWLKSNY---- 324

  Fly   279 TNPSVQAPQQNNNDIETIDDIEY-----------------PSIH---DITMKEDISAKDFRPRPD 323
             ...|.:...:||:|:  ||..|                 |||.   |.:|||.:.|
Yeast   325 -GRQVSSSFPSNNNIK--DDSVYINGNTSGLSLQHLPKMSPSIRHSMDDSMKEMLVA-------- 378

  Fly   324 FNRANKPAATRVNEQGISRPSPPAKPIAEIMRDQAEFLQRAEQNDEQLEKASKMWKRQAAEGDGL 388
                               |:    |:..:.:.|    |:...||..|:::|...|..:
Yeast   379 -------------------PT----PLNHLQQQQ----QQQSDNDHVLKRSSSFKKLFS------ 410

  Fly   389 NATEDQELHFRILQLESKAQDYIVENNRLREELSRIQELHNV------TQQLSQKEV-EATRNIE 446
            |.|.....:       |.:..|.:.:..:....|.: .||:.      :..::..|. ...:|.|
Yeast   411 NYTSPNPKN-------SNSNLYSISSLSISSSPSPL-PLHSPDPVKGNSLPINYPETPHLWKNSE 467

  Fly   447 SKIRERQRLDEQHELERQERERLLAIARETKKHYKSPTPSGPPSPGRNLEDVHVVSDSLESLLQL 511
            :.....||       |:........||....|...||:.:..|...|..:.:     |:...:..
Yeast   468 TDFMTNQR-------EQLNHNSFAHIAPINTKAITSPSRTATPKLQRFPQTI-----SMNLNMNS 520

  Fly   512 TG--DPDPTIAPNKAEIPTFDRAMKPQPRNVERTSQRVRDFSPVIGQNVGRGL-TGLKNLGNTCY 573
            .|  ....||.|:...:...|        :::.|     |.:|....|..... .||:||||:||
Yeast   521 NGHSSATSTIQPSCLSLSNND--------SLDHT-----DVTPTSSHNYDLDFAVGLENLGNSCY 572

  Fly   574 MNSILQCLSNTPQLTEYCISDKYKNYISRSNK--TNGQVIEEVAALI----KELWNGQYK-CVAS 631
            ||.|:||:..|.:||:..:.|.|..:|:.::|  :.|.:.:..|.|:    ||..:|..| .::.
Yeast   573 MNCIIQCILGTHELTQIFLDDSYAKHININSKLGSKGILAKYFARLVHMMYKEQVDGSKKISISP 637

  Fly   632 RDLRYVVGQYQKIFRGVDQQDSHEFLTILMDWLHSDLQ-----------TLHVPRQREMIS---A 682
            ...:...|....:|:...|||..||...|:|.||.||.           :.....:||.:|   |
Yeast   638 IKFKLACGSVNSLFKTASQQDCQEFCQFLLDGLHEDLNQCGSNPPLKELSQEAEARREKLSLRIA 702

  Fly   683 SEKAWLEFTKAKESMILHLFYGQMKSTVKCVACHKESATYESFSNLSLELPPNS--NVCQLNQCM 745
            |...|..|.....|:|:.||.||..|.:||..|...|.||:.|:.||:.:|..:  |...:..|.
Yeast   703 SSIEWERFLTTDFSVIVDLFQGQYASRLKCKVCSHTSTTYQPFTVLSIPIPKKNSRNNITIEDCF 767

  Fly   746 DMYFSGERI---HGWNCPSCKTKRDAIKKLDISKLPPVLVVHLKRFYADPSNSGSYMKKQN-YLR 806
            ..:...|.:   ..|.||.|:.::.:.|:|.|::||..|:||||||       .:.:.|.| ::.
Yeast   768 REFTKCENLEVDEQWLCPHCEKRQPSTKQLTITRLPRNLIVHLKRF-------DNLLNKNNDFVI 825

  Fly   807 FPLENLDMNPYIA------------------RAESRAVTPKTYQLYAVSNHYGTMEGGHYTAFCK 853
            :|. .||:.|:.|                  |.:   :.|..|:||.|:.|:||:.||||||:.|
Yeast   826 YPF-LLDLTPFWANDFDGVFPPGVNDDELPIRGQ---IPPFKYELYGVACHFGTLYGGHYTAYVK 886

  Fly   854 SANYGKWFKFDDQVVSAL-DSSNVVSSAAYILFY 886
            ......|..|||.....: :.::.::|.||:|||
Yeast   887 KGLKKGWLYFDDTKYKPVKNKADAINSNAYVLFY 920

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Usp8	NP_650948.2	USP8_dimer	2..113	CDD:286108	28/131 (21%)
		RHOD	148..278	CDD:294087	29/156 (19%)
		UCH	562..886	CDD:278850	114/370 (31%)
		Peptidase_C19R	564..887	CDD:239139	116/369 (31%)
DOA4	NP_010354.3	RHOD	208..324	CDD:197731	28/138 (20%)
DOA4	NP_010354.3	COG5533	495..924	CDD:227820	130/455 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1868
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000821
OrthoInspector	1	1.000	-	-		mtm9239
orthoMCL	1	0.900	-	-		OOG6_101021
Panther	1	1.100	-	-	O	PTHR21646
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	5.810