Protein Alignment Usp8 and USP4

DIOPT Version :10

• Sequence 1: NP_650948.2
• Gene: Usp8 / 42509
• FlyBaseID: FBgn0038862
• Length: 896
• Species: Drosophila melanogaster
• Sequence 2: NP_003354.2
• Gene: USP4 / 7375
• HGNCID: 12627
• Length: 963
• Species: Homo sapiens
• Alignment Length: 808
• Identity: 181/808 - (22%)
• Similarity: 258/808 - (31%)
• Gaps: 361/808 - (44%)

  Fly   390 ATEDQELH--FRI-LQLESKAQDYIVENNRLREELSRIQELHNVTQQ--LSQKEVEATRNIESKI 449
            ||.::|:.  |.| .:.|::..:..:.|.  .|:||:   |.|..|.  |.|.:|..
Human   163 ATIEKEMRKLFNIPAERETRLWNKYMSNT--YEQLSK---LDNTVQDAGLYQGQVLV-------- 214

  Fly   450 RERQRLDEQHELERQERERLLAIARETKKHYKSPTPSGPPSPGRNLEDVHVVSDSLESLLQLTGD 514
                 ::.|:|.....|:.|      ..|...:|:.:...||..:......||.||.:    .||
Human   215 -----IEPQNEDGTWPRQTL------QSKSSTAPSRNFTTSPKSSASPYSSVSASLIA----NGD 264

  Fly   515 PDPTIAPNKAEIPTFDRAMKPQPRNVERTSQRVRDFSPVIGQNVGRGLTGLKNLGNTCYMNSILQ 579
            ...|...:.:.:..............|..|..::.           ||.||.||||||:|||.||
Human   265 STSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQP-----------GLCGLGNLGNTCFMNSALQ 318

  Fly   580 CLSNTPQLTEYCISDKYKNYISRSNK--TNGQVIEEVAALIKELWNGQYKCVASRDLRYVVGQYQ 642
            |||||..||:|.:.|:|:..|:|.|.  ..|::.|..|.|||::|:|:...||.|..:..||::.
Human   319 CLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFA 383

  Fly   643 KIFRGVDQQDSHEFLTILMDWLHSDLQTLHVPRQREMISA--------SEKAWLEFTKAKESMIL 699
            ..|.|..||||.|.|..|:|.||.||..:......|:..|        :::||.......:|:|:
Human   384 PQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIV 448

  Fly   700 HLFYGQMKSTVKCVACHKESATYESFSNLSLELP---------------PN-------------- 735
            ..|:|..|||:.|..|.|.|.|::.|..|:|.||               |:
Human   449 DTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMG 513

  Fly   736 --SNVCQ--------------------------------LNQCM--------------------- 745
              |::|:                                ||..|
Human   514 AVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECV 578

  Fly   746 --DMYF----------------------------------------------------------- 749
              .:||
Human   579 TLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSP 643

  Fly   750 ------------------------------------SGE-------------RIHG--------- 756
                                                |||             :|.|
Human   644 LEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFT 708

  Fly   757 ----------------------------------------------------------------- 756
                                                                             
Human   709 FSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKK 773

  Fly   757 -----------------------WNCPSCKTKRDAIKKLDISKLPPVLVVHLKRFYADPSNSGSY 798
                                   |.||:||..:.|.||.|:..||.:||||||||        ||
Human   774 TTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRF--------SY 830

  Fly   799 M-----KKQNYLRFPLENLDMNPYIARAESRAVTPKTYQLYAVSNHYGTMEGGHYTAFCKSANYG 858
            .     |....:.||:..|:|:.::....:|   |..|.|.|||||||.|..|||||:.|:...|
Human   831 NRYWRDKLDTVVEFPIRGLNMSEFVCNLSAR---PYVYDLIAVSNHYGAMGVGHYTAYAKNKLNG 892

  Fly   859 KWFKFDDQVVSALDSSNVVSSAAYILFY 886
            ||:.|||..||......:|:.|||:|||
Human   893 KWYYFDDSNVSLASEDQIVTKAAYVLFY 920

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp8	NP_650948.2	USP8_dimer	2..114	CDD:462647
		RHOD	148..278	CDD:444705
		Smc	<361..>511	CDD:440809	28/125 (22%)
		Peptidase_C19R	564..887	CDD:239139	146/629 (23%)
USP4	NP_003354.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..22
		Necessary for interaction with SART3. /evidence=ECO:0000269|PubMed:20595234	27..216		16/70 (23%)
		UBP12	35..923	CDD:227847	181/808 (22%)
		Nuclear export signal. /evidence=ECO:0000250|UniProtKB:P35123	133..141
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	219..253		7/39 (18%)
		Required for USP4 activation by providing conformational flexibility between the DUSP and catalytic domains. /evidence=ECO:0000269|PubMed:25404403	229..295		13/75 (17%)
		Regulates ubiquitin dissociation. /evidence=ECO:0000269|PubMed:25404403	384..386		0/1 (0%)
		Necessary for interaction with RBL2. /evidence=ECO:0000250|UniProtKB:P35123	405..407		1/1 (100%)
		Necessary for interaction with RB1 and RBL2. /evidence=ECO:0000250|UniProtKB:P35123, ECO:0000269|PubMed:11571652	459..463		1/3 (33%)
		Interacts with DUSP and ubiquitin-like 1 domains and is required for USP4 activation. /evidence=ECO:0000269|PubMed:25404403	485..775		13/289 (4%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	637..699		4/61 (7%)
		Nuclear localization signal. /evidence=ECO:0000250|UniProtKB:P35123	767..772		0/4 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	928..963