Protein Alignment Usp8 and Usp4

DIOPT Version :10

• Sequence 1: NP_650948.2
• Gene: Usp8 / 42509
• FlyBaseID: FBgn0038862
• Length: 896
• Species: Drosophila melanogaster
• Sequence 2: NP_001128484.1
• Gene: Usp4 / 290864
• RGDID: 1587387
• Length: 961
• Species: Rattus norvegicus
• Alignment Length: 630
• Identity: 144/630 - (22%)
• Similarity: 197/630 - (31%)
• Gaps: 315/630 - (50%)

  Fly   561 GLTGLKNLGNTCYMNSILQCLSNTPQLTEYCISDKYKNYISRSNK--TNGQVIEEVAALIKELWN 623
            ||.||.||||||:|||.|||||||..||||.:.|:|:..|:|.|.  ..|::.|..|.|||::|:
  Rat   300 GLCGLGNLGNTCFMNSALQCLSNTGPLTEYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWS 364

  Fly   624 GQYKCVASRDLRYVVGQYQKIFRGVDQQDSHEFLTILMDWLHSDLQTLHVPRQREMISA------ 682
            |:...||.|..:..||::...|.|..||||.|.|..::|.||.||..:......|...|
  Rat   365 GRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKPYLEPKDANGRPDA 429

  Fly   683 --SEKAWLEFTKAKESMILHLFYGQMKSTVKCVACHKESATYESFSNLSLELP------------ 733
              :::||.......:|:|:..|:|..|||:.|..|.|.|.|::.|..|:|.||
  Rat   430 VVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIMEVFLV 494

  Fly   734 ----------------------------------------------------------------- 733
                                                                             
  Rat   495 PADPHCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPR 559

  Fly   734 -------------------------------PNSN----------------------------VC 739
                                           |:|.                            ||
  Rat   560 DDIFVYEICTTPMDGSEYITLPVYFREKKSRPSSTSSGAVLYGQPLLVSVPKHRLTLESLYQAVC 624

  Fly   740 Q---------------------------------------------------------------- 740
            :
  Rat   625 ERISRYIKQPLPEEFLSSPLEPGACNGSRGSYEGDEEEMDHQEEGKEQLSEVEESGEDSQGGDPT 689

  Fly   741 ----------------------------------------------------------------- 740
                                                                             
  Rat   690 ETTQKAKGPPRHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQES 754

  Fly   741 ---------------------LNQCMDMYFSGERI--HG-WNCPSCKTKRDAIKKLDISKLPPVL 781
                                 |.:|::::.:.|.:  |. |.||:||..:.|.||.|:..||.:|
  Rat   755 EACEKHTSMSQPQKKKKAAIALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKIL 819

  Fly   782 VVHLKRFYADPSNSGSYM-----KKQNYLRFPLENLDMNPYIARAESRAVTPKTYQLYAVSNHYG 841
            |||||||        ||.     |....:.||:..|:|:.::.   .||..|..|.|.|||||||
  Rat   820 VVHLKRF--------SYNRYWRDKLDTVVEFPVRALNMSEFVC---DRAARPYVYDLIAVSNHYG 873

  Fly   842 TMEGGHYTAFCKSANYGKWFKFDDQVVSALDSSNVVSSAAYILFY 886
            .|..|||||:.|:...|||:.|||..||......:|:.|||:|||
  Rat   874 AMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIVTKAAYVLFY 918

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp8	NP_650948.2	USP8_dimer	2..114	CDD:462647
		RHOD	148..278	CDD:444705
		Smc	<361..>511	CDD:440809
		Peptidase_C19R	564..887	CDD:239139	142/627 (23%)
Usp4	NP_001128484.1	Necessary for interaction with SART3. /evidence=ECO:0000250|UniProtKB:Q13107	27..216
		UBP12	35..921	CDD:227847	144/630 (23%)
		Nuclear export signal. /evidence=ECO:0000250|UniProtKB:P35123	133..141
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	219..257
		Required for USP4 activation by providing conformational flexibility between the DUSP and catalytic domains. /evidence=ECO:0000250|UniProtKB:Q13107	229..295
		Regulates ubiquitin dissociation. /evidence=ECO:0000250|UniProtKB:Q13107	384..386		0/1 (0%)
		Necessary for interaction with RBL2. /evidence=ECO:0000250|UniProtKB:P35123	405..407		1/1 (100%)
		Necessary for interaction with RB1 and RBL2. /evidence=ECO:0000250|UniProtKB:P35123	459..463		1/3 (33%)
		Interacts with DUSP and ubiquitin-like 1 domains and is required for USP4 activation. /evidence=ECO:0000250|UniProtKB:Q13107	485..773		4/287 (1%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	641..700		0/58 (0%)
		Nuclear localization signal. /evidence=ECO:0000250|UniProtKB:P35123	765..770		0/4 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	924..961