DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Usp8 and Usp32

DIOPT Version :9

Sequence 1:	NP_650948.2	Gene:	Usp8 / 42509	FlyBaseID:	FBgn0038862	Length:	896	Species:	Drosophila melanogaster
Sequence 2:	XP_011247292.1	Gene:	Usp32 / 237898	MGIID:	2144475	Length:	1618	Species:	Mus musculus

Alignment Length:	1461	Identity:	228/1461 - (15%)
Similarity:	388/1461 - (26%)	Gaps:	756/1461 - (51%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 LKKLHMSSNLDDLEKMSIIPDLRSKGMKILLN---TARKLYMTAEEYRLDGDEELAYITYMKYFN 65
            |.::...:..||:.::.:  || |..::.:||   |.:..::|.|:|::   ..:..:...::.|
Mouse   296 LLEVWKDNRTDDIPELHM--DL-SDIVERILNAHDTTKVGHLTLEDYQI---WSVKNVLANEFLN 354

  Fly    66 ML-----TAIHKKSDYPSHKTTVRQMLGDTESNRRIMDTLEEIINSLR-----HRYAQQHQAPEP 120
            :|     ..:..:...|..:..:.:...:.||...:.......|.|::     ..|.:...:|..
Mouse   355 LLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVV 419

  Fly   121 IAPDLVSNGRAGVDSPITQPTQYARLGLITCQDLYRRMQEKSVLVMDCRPSADYEASHLTYYCAF 185
            |.|..|.||..........|.::..                           |..:::|.|   .
Mouse   420 IEPSSVLNGGKFSFGTAAHPIEHGE---------------------------DRISNNLGY---M 454

  Fly   186 NVPEELITPGMSAGRLQARLSSSAKASWASRSVKDSVVLMDWNTKDAQPATNTAISTLLDILKNW 250
            |..||..:..:|:.. :|..|:.:...::.....|.......||.|..  ....:....:||.:.
Mouse   455 NTTEEKYSDNISSAS-EASESTGSGFLYSGTPGADMCFARQHNTSDNN--NQCLLGANGNILLHL 516

  Fly   251 DPD---------VTYRAPIQ----IVEGG-------------YE-----YFIMMYPTHCTNPSVQ 284
            :|.         :..:.|::    .:|||             ||     .:..:|..:.:|.::.
Mouse   517 NPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALP 581

  Fly   285 APQQNNNDIETIDDIEYPSIHDITMKEDISAKDFRPRPDFNRANKPAATRVNEQGI--------- 340
            .|...|:                  |.||...:..||.......:| |||..:..|
Mouse   582 RPVIKNS------------------KTDIPELELFPRYLLFLRQQP-ATRTQQSNIWVNMGMMSL 627

  Fly   341 ----------SRPSPPAKPIAEIMRDQAEFLQRAEQNDEQLEKASKMWKRQAAEGDGLNATEDQE 395
                      :.|||.| |:..::.....|              |:|             ...:|
Mouse   628 RMFPQHLPRGNVPSPNA-PLKRVLAYTGCF--------------SRM-------------QTIKE 664

  Fly   396 LHFRILQLESKAQDYIVENNRLREELSRIQELHNVTQQLSQKEVEATRNIESKIRERQRLDEQHE 460
            :|           :|:.:..|::||..|:. |:|....|:..:                 ||.|.
Mouse   665 IH-----------EYLSQRLRIKEEDMRLW-LYNSENYLTLLD-----------------DEDHR 700

  Fly   461 LER---QERERLLAIARETKKHYKSPTPSGPPSPGRNLEDVHVVSDSLESLLQLTGDPDPTIAPN 522
            ||.   |:.:.|:...|  .|....|            |::..:::|.:.              :
Mouse   701 LEYLKIQDEQHLVIEVR--NKDMSWP------------EEMSFIANSSKI--------------D 737

  Fly   523 KAEIPTFDRAMKPQPRNVERTSQRVRDFSPVIGQNVGRGLTGLKNLGNTCYMNSILQCLSNTPQL 587
            :.::||                              .:|.|||.||||||:|||.:||:|||..|
Mouse   738 RHKVPT------------------------------EKGATGLSNLGNTCFMNSSIQCVSNTQPL 772

  Fly   588 TEYCISDKYKNYISRSNK--TNGQVIEEVAALIKELWNGQYKCVASRDLRYVVGQYQKIFRGVDQ 650
            |:|.||.::...::|:|.  ..|.:.:....|::|||:|..|.||...||:.:.:|...|.|..|
Mouse   773 TQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQ 837

  Fly   651 QDSHEFLTILMDWLHSDLQTLHVPRQREMISA--------SEKAWLEFTKAKESMILHLFYGQMK 707
            |||.|.|..|:|.||.||..:|.....|:..:        :.:||....:...|:::.||:||::
Mouse   838 QDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLR 902

  Fly   708 STVKCVACHKESATYESFSNLSLELP--------------------------------------- 733
            |.|||..|...|..::.|:.|||.||
Mouse   903 SQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQL 967

  Fly   734 ----------------------------------------------------------------- 733

Mouse   968 SDLCGLKSEQILFAEVHSSNIKNFPQDNQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSP 1032

  Fly   734 ------------------------PNS-------------------------------------- 736
                                    ||:
Mouse  1033 STNGLFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKM 1097

  Fly   737 ----------------------------------------------------------------- 736

Mouse  1098 MRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDS 1162

  Fly   737 -------------------------------------------NVC------------------- 739
                                                       |.|
Mouse  1163 MGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNACIAVDWDPTALHLRYQTSQE 1227

  Fly   740 --------------------QLNQCMDMYFSGERIHGWN----CPSCKTKRDAIKKLDISKLPPV 780
                                .|:.|:..:.|.|.: |.|    |..|||...|.||||:.:|||:
Mouse  1228 RVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEEL-GENEMYYCSKCKTHCLATKKLDLWRLPPI 1291

  Fly   781 LVVHLKRFYADPSNSGSYMKKQNYLRFPLENLDMNPYI--------------------------- 818
            |::|||||   ...:|.::|.|..::||.|:.|.:.::
Mouse  1292 LIIHLKRF---QFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPTLCQHKPLTPQGDDFSELRIPA 1353

  Fly   819 --------------------------------------------------ARAESRAVTPKT--- 830
                                                              ::..|...:|:|
Mouse  1354 GDVKKVDIQSSAGEEDVLLSKSPSSLSANVTSSPKGSPSSSRKSGASCPSSKNSSPNSSPRTLGR 1418

  Fly   831 ----------------------------------------------------------------- 830

Mouse  1419 NKGRLRLPQIGSKNKLSNSKENLDTSKENGAGQICELADTLNRRHVLGGSQPELVTPLDHEITLA 1483

  Fly   831 ----------------------------------------YQLYAVSNHYGTMEGGHYTAFCKSA 855
                                                    |.|||:|.|.|.:.||||..:.|:.
Mouse  1484 NGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREETHSKPIYNLYAISCHSGILGGGHYVTYAKNP 1548

  Fly   856 NYGKWFKFDDQVVSALDSSNVVSSAAYILFY 886
            |. ||:.::|.....|....:.:.:||||||
Mouse  1549 NC-KWYCYNDSSCKELHPDEIDTDSAYILFY 1578

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Usp8	NP_650948.2	USP8_dimer	2..113	CDD:286108	20/121 (17%)
		RHOD	148..278	CDD:294087	23/160 (14%)
		UCH	562..886	CDD:278850	130/835 (16%)
		Peptidase_C19R	564..887	CDD:239139	131/835 (16%)
Usp32	XP_011247292.1	FRQ1	189..339	CDD:227455	11/45 (24%)
		UBP12	518..1330	CDD:227847	160/949 (17%)
		UCH	<1248..1578	CDD:366104	55/334 (16%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1591
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	1.030