DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Calx and slc8a4b

DIOPT Version :9

Sequence 1:	NP_001247215.1	Gene:	Calx / 42481	FlyBaseID:	FBgn0013995	Length:	973	Species:	Drosophila melanogaster
Sequence 2:	NP_001116712.1	Gene:	slc8a4b / 557499	ZFINID:	ZDB-GENE-050809-103	Length:	925	Species:	Danio rerio

Alignment Length:	942	Identity:	438/942 - (46%)
Similarity:	592/942 - (62%)	Gaps:	121/942 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    93 DAEELRE----------CSEGLVLPLWMPQRNISVGDRLVRGFVYFVLLIYLFVGVSIIADRFMA 147
            ||::|.:          |::|::||:|.|| |.|||| :.|..||.|.|:|:|:|:||||||||.
Zfish    33 DAQDLDQSTSNCSQNDVCTDGVLLPVWNPQ-NPSVGDXVARAIVYLVALVYMFLGMSIIADRFMT 96

  Fly   148 AIEAITSIERAVVVKGPNNTKQVMHVRIWNETVANLTLMALGSSAPEILLSVIEIYAKDFESGDL 212
            |||.|||.|:.:..|.||.......||||||||:||||||||||||||||||||:...:||:|.|
Zfish    97 AIEVITSQEKEITTKRPNGETVTTTVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHNFEAGSL 161

  Fly   213 GPGTIVGSAAYNLFMIIAVCMIWIPAGEVRRIRHLRVFFVTALFSVFAYVWLWLILSVFTPGVIL 277
            ||.|||||||:|:|:||.:|:..:|.||.|:::|||||||||.:|:|||:||:|||:|.:||.:.
Zfish   162 GPSTIVGSAAFNMFVIIGLCVYVVPEGERRKVKHLRVFFVTAAWSMFAYIWLYLILAVISPGEVE 226

  Fly   278 VWEAIVTLLFFPLTVLWAYIAERRLLVYKYMDKNYRVNK-RGTVVA--GEH----------DQVE 329
            ||||::|.|||||.|:.|:||:||||.|||..|.||.:| ||.:|:  ||.          |.:|
Zfish   227 VWEAVLTFLFFPLCVVQAWIADRRLLFYKYARKRYRTDKGRGIIVSEGGEELGKEAGFTKMDMLE 291

  Fly   330 MD-----------AEKGPKQPMVTSARGNDAEAFDEARREYITLLTELRQKYPDADLEQLEMMAQ 383
            :|           .|.|        ..|.|.|  :|||||....|.||:|::|:.|:|||..||.
Zfish   292 IDGITTHLDGVLGGESG--------LGGRDQE--EEARREMARTLKELKQRHPEKDMEQLIEMAN 346

  Fly   384 EQVLARSSKSRAFYRIQATRKMVGSGNLMRKIQERAHSDLTEVKAQLHAGDD-----------EE 437
            .|||.:..||||||||||||.|:|:||:::|                ||.|.           |:
Zfish   347 YQVLMQQQKSRAFYRIQATRMMIGAGNILKK----------------HAADQARKVVSSGEPREQ 395

  Fly   438 ADDP--IRMYFEPGHYTVMENCGEFEVRVVRR-GDISTYASVEYETQDGTASAGTDFVGRKGLLS 499
            .|||  .|:.|||..|...||||..::.|.|. ||......|:|.|:||||:||:|:...:|.|.
Zfish   396 EDDPHVTRIDFEPALYQCFENCGSLKLTVQRHGGDAGCSVKVDYRTEDGTANAGSDYEFAEGTLV 460

  Fly   500 FPPGVDEQRFRIEVIDDDVFEEDECFYIRLFNP-----SEGVKLAVPM-------------IATV 546
            |.||.:.:...:.:||||:|||||.||:||.||     ::|..:|:..             .|||
Zfish   461 FKPGENVKDIAVGIIDDDIFEEDEYFYVRLSNPRIVGWADGHPIALETGAPAPSAALGEAHTATV 525

  Fly   547 MILDDDHAGIFAFTDSVFEITESVGRFELKVMRYSGARGTVIVPYWTENDTATESKDYEGARGEL 611
            .|.||||||||.|......::||:|..::||.|.|||||...|||.|...||...:|||...|:|
Zfish   526 TIYDDDHAGIFTFESESMRVSESIGIMQVKVQRTSGARGLAAVPYQTVEGTACGGEDYEEVSGKL 590

  Fly   612 VFENNESEKFIDLFILEESSYEKDVSFKVHIGEPRLAPDDTNKESFFNRFFEKHEHSGEPTHDEL 676
            .|:|:|:.|.|::.|:::..|||:.:|.:.:|||.|.           ...:||..|.| ...|:
Zfish   591 EFQNDETMKTIEVKIIDDEEYEKNKTFSIELGEPVLL-----------EIGQKHGDSNE-NKPEI 643

  Fly   677 AAKIKEVEKKPVQDLTELDRILLLSKPRNGELTTAYVRIRESQEFKATVDKLVAKANVSAVLGTS 741
            .|:.:||.|              :..|..||.|...|.|.||.|||.|||||:.|.|::.|:|:|
Zfish   644 GAEEEEVAK--------------MGCPSLGEHTRLEVVIEESYEFKNTVDKLIKKTNLALVVGSS 694

  Fly   742 SWKEQFKDALTVIPADESEFDNDDEEEEVPSCFSYVSHFVCLFWKVLFAFVPPTDICGGYVTFVV 806
            ||:|||..|:||...|:.|  .:..||.:||||.|:.||:.:||||||||||||:...|:..|:|
Zfish   695 SWREQFVSAVTVSAGDDDE--EESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFIV 757

  Fly   807 SIFVIGVITAIIGDAASYFGCALNIKDSVTAILFVALGTSIPDTFASMIAAKHDEGADNCIGNVT 871
            |||:||.:||:.||.||:|||.:.:||||||::|||||||:||||||.:||..|:.||..|||||
Zfish   758 SIFLIGALTAVTGDLASHFGCTVGLKDSVTAVVFVALGTSVPDTFASKVAAIQDQYADASIGNVT 822

  Fly   872 GSNAVNVFLGIGLAWTIAAVYHSSHGMTFNVEPGTIGFAVALFCGEALIAIMLIMFRRWHKGIGA 936
            ||||||||||||:||:|||:|..|.|.:|:|:||::.|:|.||...|::.:.::::||.....|.
Zfish   823 GSNAVNVFLGIGVAWSIAAIYWRSKGKSFHVDPGSLAFSVTLFTALAVVCVSVLLYRRRPSVAGG 887

  Fly   937 ELGGPKVSKYISAAILVFLWVFYVVICILEAY 968
            |||||:..|.:::.:.:.||:.|:::..||.|
Zfish   888 ELGGPRTCKILTSLLFISLWLIYILLASLETY 919

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Calx	NP_001247215.1	caca	4..973	CDD:273296	438/942 (46%)
		Na_Ca_ex	126..297	CDD:279963	108/170 (64%)
		Na_Ca_ex_C	300..414	CDD:293103	59/137 (43%)
		Calx_beta	441..529	CDD:197594	40/90 (44%)
		Calx_beta	554..643	CDD:197594	37/88 (42%)
		Na_Ca_ex	800..965	CDD:279963	87/164 (53%)
slc8a4b	NP_001116712.1	caca	8..925	CDD:273296	438/942 (46%)
		Na_Ca_ex	75..242	CDD:279963	106/166 (64%)
		Na_Ca_ex_C	249..380	CDD:293103	61/156 (39%)
		Calx-beta	401..491	CDD:281194	40/89 (45%)
		Calx_beta	533..622	CDD:197594	37/88 (42%)
		Na_Ca_ex	751..916	CDD:279963	87/164 (53%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170592426
Domainoid	1	1.000	244	1.000	Domainoid score	I2153
eggNOG	1	0.900	-	-		E1_KOG1306
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	816	1.000	Inparanoid score	I460
OMA	1	1.010	-	-		QHG59599
OrthoDB	1	1.010	-	-		D109488at33208
OrthoFinder	1	1.000	-	-		FOG0000830
OrthoInspector	1	1.000	-	-		otm24329
orthoMCL	1	0.900	-	-		OOG6_101777
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X483
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	13	12.670