Protein Alignment Nlg4 and cest-4

DIOPT Version :10

• Sequence 1: NP_001369025.1
• Gene: Nlg4 / 42402
• FlyBaseID: FBgn0083975
• Length: 1437
• Species: Drosophila melanogaster
• Sequence 2: NP_501043.1
• Gene: cest-4 / 177445
• WormBaseID: WBGene00015930
• Length: 592
• Species: Caenorhabditis elegans
• Alignment Length: 705
• Identity: 136/705 - (19%)
• Similarity: 230/705 - (32%)
• Gaps: 233/705 - (33%)

  Fly     4 SQLLLLLRLLLLPTVLISWMHCAGASTADIYKGARLGHRIVQTRYGRLHGLILPLDSFRFLRSVE 68
            :.|||.|...||               ..|.|.|     ||.|..|::.|     |.   :.|..
 Worm     9 NNLLLFLSFFLL---------------FSITKSA-----IVSTSLGQIDG-----DQ---IGSFH 45

  Fly    69 VFLGVPYATPPTKQNRFSPTRAPAPWDGIRISDKYSPVCPQRLPNIQNETAALEKMPKGRLEYLK 133
            :|..:|.|.||..:.||.....|..|.|:|.:..|.|.|      |.|  :::...|:..::
 Worm    46 LFKHIPLAKPPIGKLRFQKPEPPEKWTGVRNAKAYGPAC------ISN--SSVSTSPQKWID--- 99

  Fly   134 RLLPFLENQSEDCLYLNVFSPVNAGANEKKLPVIVFIHGESFEWSSGNPYDGSVLAS-------- 190
                      ||||::|:|:. :.....|...|:.:.||      .|..||.:|:.:
 Worm   100 ----------EDCLHINIFTS-DKCLKSKDCAVVTYFHG------GGLMYDSAVMFNDTYLLGTF 147

  Fly   191 -YGEVVVVTLNYRLGILGFLNANPNPHAHARVANYGLMDQMAALHWIQQNIQKFGGDPNSVTLAG 254
             ..:|::....:||||...........|...:|.|   |.:..:.:::..|..|||:....|:.|
 Worm   148 VNQDVIIAIPAFRLGIFSHFVVEDQSIAPTNLALY---DILLGVEFVKNEIHNFGGNNQKNTIMG 209

  Fly   255 HGTGAACINYLMTSPTMVR--GLFHRAILMSGSAYSSWALVEDPVLFAIKLAKEVNCTIPDDINR 317
            |..|.:.:|.|..|..:..  .||.::|.||..  ..:..:|..:....:.||...||:|
 Worm   210 HSYGGSIVNILTFSSKINHDLSLFQQSICMSSG--HDFETLEIQIQKTNRFAKHAGCTVP----- 267

  Fly   318 HHEQIVDCLRDVPLEDLY-LADIQAPNFLTSFGPSVDGVVIRPGHSNLDIDDLMARNSRRSSADS 381
               .|::........||| :..:|                      |.|..:|:  ..:|...|.
 Worm   268 ---SIIEKKMTTRQSDLYRMKCLQ----------------------NKDALELL--RVQRLLEDE 305

  Fly   382 GFQSSAGGGGGQGGGAGGGGGGGSGSSFGGGYFGGSGAGTMNMGGHYDVLFGVVTGESIWRFSAH 446
            |:.:.                         ||                    :|..|.:  ....
 Worm   306 GYPTY-------------------------GY--------------------LVQREPL--IQEI 323

  Fly   447 DIQNGFEGERRDKIIRTYVR-------------NAYNYHLNEIFYTIVNEYTDWDRTSQHPINTR 498
            ..|...:..::..::....|             .|..:...|..|....:  |||.......|..
 Worm   324 PYQEFMKSPKKIPMLTGCTRYEMDHSPAPKPIGQALGFENPEEVYMKFRK--DWDDEVYEFGNHS 386

  Fly   499 DTAVAALSDAQFVAPIVRAGDILAANSPPPVSSSSTAGSPGANAAASTSAGSTQPSGRCYFYVFD 563
            |...|.:...:     ||..::|:.|.|.                                |:::
 Worm   387 DETQAIMVQVR-----VRVNNLLSKNVPA--------------------------------YLYE 414

  Fly   564 YQTKDGDYPQRMGTVHGEDLPYIFGAPLVDGFSHFPQNYTKSETALSEAVMIFWTNFARTGNPNE 628
            |     .||:.  ..|.:||.|:.|.      ..|.::  ::|..|.:.....:.|||:.|:|.|
 Worm   415 Y-----TYPKH--ARHTDDLFYLMGV------HRFEKD--ENEIELGKVYETIFMNFAKFGDPKE 464

  Fly   629 HHRQDSSLPVSKERNRFRSITWENYDPLHQKYLEIGMKPRIKNHFRAHQLSIWLR 683
            ....     .:.|...:..:.|:.         |.|.:|::|..|....::.||:
 Worm   465 GFEL-----ANAENESYYEVYWDG---------ETGERPQMKTKFEEKIINYWLK 505

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nlg4	NP_001369025.1	COesterase	43..681	CDD:395084	125/662 (19%)
cest-4	NP_501043.1	alpha/beta hydrolases	26..503	CDD:473884	126/669 (19%)