DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ask1 and map3k5

DIOPT Version :9

Sequence 1:	NP_477089.3	Gene:	Ask1 / 42366	FlyBaseID:	FBgn0014006	Length:	1363	Species:	Drosophila melanogaster
Sequence 2:	NP_001155222.1	Gene:	map3k5 / 555703	ZFINID:	ZDB-GENE-030827-3	Length:	1364	Species:	Danio rerio

Alignment Length:	1353	Identity:	552/1353 - (40%)
Similarity:	768/1353 - (56%)	Gaps:	164/1353 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 ALEEIKQAVQSAGANFQRVQFERLDFGETNVLETFYNADVAIIDLSILTQQRPLSYHYGVRESFG 85
            ||:.:|.|....|.....|.|.:||||||:||:.|||||:|:::|:...:|..|.||.||||||.
Zfish    99 ALQCLKDACDIVGCKLDVVPFGKLDFGETSVLDHFYNADIAVVELTDAFRQPSLFYHLGVRESFC 163

  Fly    86 MKENILTYNDIDSKQTLSLK-------LSC-ANYLFLSYKRNAETN-SCHLTSQPNSGNNSKEPN 141
            |..||:.|.|.:|....||:       ::| |||.|:.|....::. .|..:|.....:...:|:
Zfish   164 MVNNIILYCDHNSDSLQSLQDLICQKNMTCAANYTFIPYMVTPQSKVYCCESSLMKGLSELMQPS 228

  Fly   142 AEGR-----VPTLQWRLKRKLQDVEIQSKAHMREKFLSDMRTARDTYATNGAKLQSILHEMRKRL 201
            .|..     :|.|. ||.:.|...:..:..:.||..|:::|.||:.|  .||:|.:.|..:::||
Zfish   229 FEALLGPICMPLLD-RLTQLLTGCKSNTCQYFRETVLNEIRKARELY--TGAELAAELRRIQQRL 290

  Fly   202 DDPHVLSGEVVHSFMCSLRDVQDYDAMVRLVNDLKNIPNTRKYVETGNMSFLYAFALNRRNRKGD 266
            |:...||.:||.:.:.|.||:|||:::|:||..|:.:| |...|...::.|.|||||||||..||
Zfish   291 DNVECLSADVVINLLLSYRDIQDYESIVKLVETLEKLP-TFDPVAHPHIKFHYAFALNRRNLPGD 354

  Fly   267 REKALESSLKALEKKENHFPDMLCLCGRIYKDIFVESDYTDATSLAHAIKWYRQSFEVQPNEYAG 331
            |:|||:..|..:...|....|:.||.||||||:|::|.:||..|....|.|:|:.||.:|..::|
Zfish   355 RQKALDIMLPLVNSDEQVASDIYCLVGRIYKDMFLDSHFTDTNSRDQGIHWFRRGFESEPTLHSG 419

  Fly   332 INLATLLVIEGKEFTNTEELQHIGMTLNNLIGKKGSLSSLSEYWDVATFFEISVLANDYAKAIQA 396
            ||.|.||:..|.:|.::.||:.:|:.|::|:||||||..|..||||..|...|:||.|..:.|||
Zfish   420 INYAVLLLAAGHQFDSSFELRKVGVKLSSLLGKKGSLDRLQSYWDVGFFLGASILACDNTRVIQA 484

  Fly   397 AECMFKLKPPNWYLKSTIGNISLIHRFRKKPEERQPPIEEQVFQFWMDFFLEATNTEEVKNSIRF 461
            :|.:||:|.|.|||:|.:..|.:...|.|...:.|.|.:|.| .|||||.:||||.::...|:||
Zfish   485 SEKLFKIKAPIWYLRSLVETILIYKHFTKVSTDPQAPKQELV-DFWMDFMVEATNKDKDVTSVRF 548

  Fly   462 PILILEPQKIYMPSYVTINMDADEKSIQIVNICLAHAKNACKKIHDFLFVASKIKSVSLYKRDDR 526
            |:|||||.|:|.|||::||.||:|.::.|.::    ..:..|.||::.|.|:.::.||:.|.|:|
Zfish   549 PVLILEPTKVYQPSYLSINKDAEENTLSIWHV----TPDDKKGIHEWNFSAASVRGVSISKFDER 609

  Fly   527 CAYLYVHHNSDDFQIYFPSTERRQKFYDMILEMTADQVVFVNLSNDDAN------IEYEYDYDEQ 585
            ..:|||.:||:||||||.:....::|.|::..:|.:.     ....|.|      :||:|:|||.
Zfish   610 SCFLYVVNNSEDFQIYFCTEMHCKRFCDLVNTVTEEA-----WKGSDENECESEALEYDYEYDEH 669

  Fly   586 NRKMVLGKGTYGTVYAARDKQTQVRIAIKEVPEKNSQDVQPLHEEIKLHSQLRHRNIVRYLGSCS 650
            ..|:||||||:|.|||.||...|||:||||:||::|:..|||||||.||..|:|:|||:||||.|
Zfish   670 GEKVVLGKGTFGVVYAGRDLSNQVRLAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSIS 734

  Fly   651 ENGFFKIFMEQVPGGSLSDLLETKWGPLKDNESTMAFYSKQILEGLKYLHEQDIVHRDIKGDNVL 715
            ||||.|||||||||||||.||.:||||||:||.|:.||:||||:||||||:..||||||||||||
Zfish   735 ENGFIKIFMEQVPGGSLSALLRSKWGPLKNNEPTIGFYTKQILDGLKYLHDNQIVHRDIKGDNVL 799

  Fly   716 VNTYSGVVKISDFGTSKRLARINPMTETFTGTLQYMAPEVIDQGVRGYGPAADIWSFGCTNVEMA 780
            :||||||:||||||||||||.|||.||||||||||||||:||:|.||||..|||||.|||.:|||
Zfish   800 INTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTIIEMA 864

  Fly   781 TGKPPFIELGSAHAAMFKVGFYKKHPNIPEELSANAKNFILRCFAISVMDRPSASQLLEDPFLQD 845
            ||||||.|||...|||||||.:|.||.||:.:|:.||.||||||......|.:|:.||...||..
Zfish   865 TGKPPFYELGEPQAAMFKVGMFKIHPEIPDSMSSEAKAFILRCFEPDPDSRATANDLLTHEFLTV 929

  Fly   846 KPRKVR------PALPINTEFGRSISVPADRFVHKTTPPLSY------NTTCNTPTT--PELDIT 896
            ..||.|      .||...:|:.||||:|....|..|:....|      |..|..|..  |.
Zfish   930 TSRKKRSKTSSFTALTPGSEYLRSISLPVPVVVEDTSSSSEYGSVSPDNELCTNPFNFRPS---- 990

  Fly   897 HSSSVDIDELPSNQFMLERRNSHGFLLSPEIE-------PSTPSLRTSISETSETDGFYRLKKDS 954
             |||....|       .|.:......||..:|       |.:|..:.|        ||:.|||||
Zfish   991 -SSSKSYSE-------REVKGHRSLFLSIPVENFEDHSAPPSPEEKDS--------GFFMLKKDS 1039

  Fly   955 QRRTTLSKVLALDESKICDIWLKKVDADQNSIAIRKSDLEVLIRGLRDYIMNENEKHLEATINEL 1019
            :||.||.::|..|..||....|:.:........::...:..|:..|.|::...:.|.:.:|:::|
Zfish  1040 ERRATLHRILTEDREKIVLHLLEALTQGSEETKLKHQHISTLVASLGDFVRMADRKIIASTLSQL 1104

  Fly  1020 KQKLNNDAVALDHLHLALYSFQDAVVCVLRYHCIKPHWMFALDNLVKRAVQAAVTIFSPEL---- 1080
            |.:|:.|:.|:..|.:||:.|||||..|||.|.|||||||||||::::|||.|:.|..|||
Zfish  1105 KLELDFDSTAISQLQVALFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAIVILVPELRPHF 1169

  Fly  1081 --------------------GANLADKDLS----GNDDESLRTSLMQKGSTESQEKQSLEKVLPT 1121
                                ..|.|.||::    .:||....:.:....||.|.|.||.::.:..
Zfish  1170 TLASESDPAEQEDIDDDAVPQGNAALKDMAPALVHHDDTVATSGVSTLSSTVSHESQSAQRSISM 1234

  Fly  1122 SNTREGVGLTPAESGGDALSSSMECARILRDIRDNQQKLQ---RQLLEQQRQSMRALHNISQELA 1183
            ...|                ..:|..|:|.::.:.:::.|   :|:||.:.|.::.|
Zfish  1235 ELGR----------------MKLETKRLLEELVEKEREYQAILQQVLEDREQEIKLL-------- 1275

  Fly  1184 HIYMGGRKRLRRTINGFNKKCHRMSTSVGGTSGV-RPNIAGNPRRQLNKHHFGLHEVDEQLEQWL 1247
                               ||.        |..: .|....|.|..|:      .:.|.||.:||
Zfish  1276 -------------------KCR--------TGPIDAPVQLVNTRSSLD------GDGDLQLLEWL 1307

  Fly  1248 TQQEIDEFSKTLILNEGFTFDDFIYNMEKLDLMRLGLRVGIEVRLWKLIIQER 1300
            .....|..|...|:.|.::.||.::.:.:.||..|.||.|:..:||..|.:.|
Zfish  1308 KLHGADADSIERIIAEDYSLDDILHYVTRDDLKTLRLRGGVLCKLWSAITEHR 1360

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ask1	NP_477089.3	DUF4071	74..444	CDD:290020	151/383 (39%)
		STKc_ASK	576..843	CDD:270794	187/266 (70%)
		S_TKc	590..843	CDD:214567	180/252 (71%)
map3k5	NP_001155222.1	DUF4071	152..531	CDD:290020	151/383 (39%)
		STKc_ASK	660..927	CDD:270794	187/266 (70%)
		S_TKc	674..927	CDD:214567	180/252 (71%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170593558
Domainoid	1	1.000	377	1.000	Domainoid score	I819
eggNOG	1	0.900	-	-		E1_KOG4279
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	963	1.000	Inparanoid score	I312
OMA	1	1.010	-	-		QHG45642
OrthoDB	1	1.010	-	-		D122434at33208
OrthoFinder	1	1.000	-	-		FOG0001687
OrthoInspector	1	1.000	-	-		otm24261
orthoMCL	1	0.900	-	-		OOG6_104013
Panther	1	1.100	-	-	O	PTHR11584
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1591
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.770