Protein Alignment Ask1 and nsy-1

DIOPT Version :9

• Sequence 1: NP_477089.3
• Gene: Ask1 / 42366
• FlyBaseID: FBgn0014006
• Length: 1363
• Species: Drosophila melanogaster
• Sequence 2: NP_001370698.1
• Gene: nsy-1 / 24104671
• WormBaseID: WBGene00003822
• Length: 1498
• Species: Caenorhabditis elegans
• Alignment Length: 1457
• Identity: 590/1457 - (40%)
• Similarity: 809/1457 - (55%)
• Gaps: 219/1457 - (15%)

  Fly     1 MDVVCVMDTAVGDHLEDRQCALEEIKQAVQSAGANFQRVQFERLDFGETNVLETFYNADVAIIDL 65
            :.||.|:|..|..:|..|:.||:::::...:...|..|:.|::||||||..|:.|||||||::|:
 Worm    63 LHVVIVIDQKVQKNLRVREMALKDVQKVADTLNVNLTRIDFDKLDFGETETLDLFYNADVALVDV 127

  Fly    66 SILTQQRPLSYHYGVRESFGMKEN-ILTYNDIDSKQTL--SLKLSCANYLFLSYKRNAETNSCHL 127
            ::..||..|.||.|||||.|...| ||||...|.:..:  :||.:.|:...:.|..:.::|....
 Worm   128 TVTHQQPSLCYHIGVRESMGQSYNMILTYWSPDPEYHIMDALKKTHAHLPMIVYIHHQDSNQLQS 192

  Fly   128 TSQPNSGNNSKEPNAEGRVP----TLQWRLKRKLQDVEIQSKAHMREKFLSDMRTARDTYATNGA 188
            ..:.|:.::||.|.|...||    |.|.|:|:.|:.|::::.||.||||:||:|.||:  .|:|.
 Worm   193 YDKNNNDDDSKPPFARTNVPAKTITFQHRMKQVLKSVQVEASAHSREKFMSDLRKARE--ITDGD 255

  Fly   189 KLQSILHEMRKRLDDPHVLSGEVVHSFMCSLRDVQDYDAMVRLVNDLKNIPNTRKYVETGNMSFL 253
            :....|.:||.|||:|.||..:.|...|.|.||.|:|..|:|||:|||.||:..|.|:|..:.:.
 Worm   256 QKNDYLDKMRTRLDNPDVLHPDTVSLMMLSYRDNQNYGGMIRLVDDLKRIPDCLKVVDTPVIRYQ 320

  Fly   254 YAFALNRRNRKGDREKALESSLKALE-KKENH------FPDMLCLCGRIYKDIFVESDYTDATSL 311
            |||||||||:.|||:.||.:.|..:| ..||.      .||::||.||||||.|:.|:|.|..||
 Worm   321 YAFALNRRNKDGDRDLALNTVLSLVEGTTENEEKNGPLSPDVVCLAGRIYKDKFIASNYEDRESL 385

  Fly   312 AHAIKWYRQSFEVQPNEYAGINLATLLVIEGKEFTNTEELQHIGMTLNNLIGKKGSLSSLSEYWD 376
            ..||:|||::||:.|.||:||||.|||...|:.|.|..|:|.|.:.||:|:|:||:|.:|.||||
 Worm   386 NSAIEWYRRAFEMSPLEYSGINLTTLLRASGEHFENNLEMQQIAVVLNSLLGRKGALQNLMEYWD 450

  Fly   377 VATFFEISVLANDYAKAIQAAECMFKLKPPNWYLKSTIGNISLIHRFRK--KPEERQPPIEEQVF 439
            |||:||:||||.:|.||.:||..|.|||||.||||||:.||.||:|...  .|.|:    |:|.|
 Worm   451 VATYFEVSVLAENYQKACEAALMMVKLKPPVWYLKSTMENIKLINRCAATISPIEK----EKQQF 511

  Fly   440 QFWMDFFLEATNTEEVKNSIRFPILILEPQKIYMPSYVTINMDADEKSIQIVNICLAHAKNACKK 504
            .||.:||:|||..:...:..|:|:||||..|.:.|||:|:|   :|:...|::..|.:::.  ||
 Worm   512 LFWSEFFMEATEADTDISCPRYPVLILELNKEFTPSYLTLN---NEEGTVILSHVLENSQQ--KK 571

  Fly   505 IHD--------FLFVASKIKSVSLYKRDDRCAYLYVHHNSDDFQIYFPSTERRQKFYDMILEMTA 561
            ||.        :.|..:.||:|:..|||||..:||||.|||||.:.||:....:|.||.:..| |
 Worm   572 IHQSELRGIHRWHFARNNIKAVTESKRDDRQLFLYVHENSDDFNLLFPTKAHCKKAYDDMKSM-A 635

  Fly   562 DQVVFVN-----LSN-DDANIEYEYDYDEQNRKMVLGKGTYGTVYAARDKQTQVRIAIKEVPEKN 620
            | |...|     ||| |:..|.:||:....|.::|||||||||||||||..||.:|.:||:..|.
 Worm   636 D-VADGNYQGRVLSNPDNEKIRFEYELSNSNERVVLGKGTYGTVYAARDMDTQRQIVVKEIEVKY 699

  Fly   621 SQDVQPLHEEIKLHSQLRHRNIVRYLGSC------SENGFFKIFMEQVPGGSLSDLLETKWGPLK 679
            .::||||.|||.|||.|.|.|||:||| |      ..|..|.||||.|||||||.||.:||||:
 Worm   700 DEEVQPLMEEISLHSTLCHANIVQYLG-CDLVGKDGSNDHFLIFMEHVPGGSLSSLLRSKWGPM- 762

  Fly   680 DNESTMAFYSKQILEGLKYLHEQDIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLARINPMTETF 744
             ||:.|.:|.||||||||||||..|||||||||||||||||||.|||||||.||||.:||:||||
 Worm   763 -NENAMNYYGKQILEGLKYLHELKIVHRDIKGDNVLVNTYSGVCKISDFGTCKRLAGLNPVTETF 826

  Fly   745 TGTLQYMAPEVIDQGVRGYGPAADIWSFGCTNVEMATGKPPFIELGSAHAAMFKVGFYKKHPNIP 809
            |||||||||||||.|.||||..|||||||||.||||||:|||:|:.:..||||:||.:|.||.||
 Worm   827 TGTLQYMAPEVIDHGQRGYGAPADIWSFGCTMVEMATGRPPFVEMQNPQAAMFRVGMFKTHPPIP 891

  Fly   810 EELSANAKNFILRCFAISVMDRPSASQLLEDPFLQDKPRKV----------RPALPI-------- 856
            .|::...:|||..||.....|||||..||:|||:......:          :||..|
 Worm   892 TEITEKCRNFIKSCFLPEACDRPSAKDLLQDPFIYHNHHSISRTRSGSINKKPATKIELNHDKEK 956

  Fly   857 ------NTEFGRSISVPADRFVHKTTPP---------------LSYNTTCNTPTTP------ELD 894
                  ..|..||.|......|.:.:||               :...|..|..:|.      .|.
 Worm   957 KEKSKNQREMLRSTSHIGGMGVVERSPPTPEPMSATLTAGFSHVHSQTVSNALSTAREEKKLHLK 1021

  Fly   895 ITH------SSSVDIDELPSNQFMLERRNSH-GFLLSPEIEPSTP---------------SLRT- 936
            |.|      |||   ..:|..|.......|| ||.||   :||:|               ||.|
 Worm  1022 IDHARNRTFSSS---SPVPDGQSSAGTNMSHPGFQLS---QPSSPIVDDTNHPHLIVSPISLNTM 1080

  Fly   937 ------------SISETSETDG--FYRLKKDSQRRTTLSKVLALDESKICDIWLKKVDADQNSIA 987
                        :||:.|....  |:.|:|||:||.:|.:.:...:..|.|.|        :::.
 Worm  1081 GSPLSSAALLNRTISDESSNSSSRFFMLQKDSERRRSLGQFMQDYKDLIIDSW--------STLL 1137

  Fly   988 IRKSD---------LEVLIRGLRDYIMNENEKHLEATINELKQKLNNDAVALDHLHLALYSFQDA 1043
            |::||         ||:|:.|:||:::.::...::..|::::..|:.|...:..::||||.|.|:
 Worm  1138 IKQSDTELVVTVYMLEMLLDGMRDFLLKKDNTKMQKMIDDIRGLLDYDTAKIGQINLALYHFSDS 1202

  Fly  1044 VVCVLRYHCIKPHWMFALDNLVKRAVQAAVTIFSPELGANLADKDLSGNDDESLRTSLMQKGSTE 1108
            :..|||...|||||||||.||:..|||.|::|.||:|...|..:|           :|....|..
 Worm  1203 IQPVLRRLDIKPHWMFALSNLITSAVQCAISILSPDLSLLLHAQD-----------NLPSTSSIV 1256

  Fly  1109 SQEKQSLEKVLPTSNTREGVGLTPAESGGDALSSSMECARILRDIRDNQQKLQRQLLEQQRQSMR 1173
            :....||                   |.|:||..|...:|..| :|:::::| |.|.|:....:.
 Worm  1257 AIRNSSL-------------------SEGEALIESRPPSREER-VREDRKEL-RTLQEENEILIE 1300

  Fly  1174 ALHNISQELAHIYMGGRKR---------LRRTINGFNKK--CHR-----MSTSVG----GT-SGV 1217
            .|..:.:||......|..|         .|.|...|...  .|.     .|.|.|    || :..
 Worm  1301 RLLQVERELNAQLKSGITRANRFRDFAMYRNTYPPFRTPPVAHAPPTPPFSASCGAQPSGTFTNQ 1365

  Fly  1218 RPNIAG-NPRRQL------NKHHFGLHEVDEQLEQWLTQQEIDEFSKTLILNEGFTFDDFIYNME 1275
            .|:.|. .|..|.      .::::.:..|.|:|..||...||||.|..||.:|.:|..|.:..:.
 Worm  1366 PPSFASIKPIAQKIIMPPGTENNYQVTRVQEELVSWLRGLEIDERSIALIASEAYTKSDMMDFVT 1430

  Fly  1276 KLDLMRLGLRVGIEVRLWKLI--IQER 1300
            :.:|:.:|:..|...|:.:.|  ::||
 Worm  1431 RDELLSIGVGGGSSCRIMRAIGEVRER 1457

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ask1	NP_477089.3	DUF4071	74..444	CDD:290020	181/385 (47%)
		STKc_ASK	576..843	CDD:270794	178/272 (65%)
		S_TKc	590..843	CDD:214567	174/258 (67%)
nsy-1	NP_001370698.1	DUF4071	136..517	CDD:404208	181/386 (47%)
		ASK_PH	529..631	CDD:408809	42/106 (40%)
		STKc_ASK	655..925	CDD:270794	178/272 (65%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160160050
Domainoid	1	1.000	348	1.000	Domainoid score	I547
eggNOG	1	0.900	-	-		E1_KOG4279
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	879	1.000	Inparanoid score	I203
Isobase	1	0.950	-	0	Normalized mean entropy	S2727
OMA	1	1.010	-	-		QHG45642
OrthoDB	1	1.010	-	-		D122434at33208
OrthoFinder	1	1.000	-	-		FOG0001687
OrthoInspector	1	1.000	-	-		oto17488
orthoMCL	1	0.900	-	-		OOG6_104013
Panther	1	1.100	-	-	LDO	PTHR11584
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3835
SonicParanoid	1	1.000	-	-		X1591
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.750