DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG4562 and cftr

DIOPT Version :9

Sequence 1:	NP_001262747.1	Gene:	CG4562 / 42362	FlyBaseID:	FBgn0038740	Length:	1408	Species:	Drosophila melanogaster
Sequence 2:	NP_001038348.1	Gene:	cftr / 559080	ZFINID:	ZDB-GENE-050517-20	Length:	1485	Species:	Danio rerio

Alignment Length:	1547	Identity:	454/1547 - (29%)
Similarity:	742/1547 - (47%)	Gaps:	264/1547 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LPENPREKSNIFSSLMFCFAMPTFFKGRKKTLDENDLYRALQEHRSDHLGSKLSAAWEKEVEKKR 73
            :..:|.|.:|..|...|.:..|...||.|:.|..:|:|:|..:..:|.|..:|...|::||...:
Zfish     1 MQRSPVEDANCLSRYFFWWTNPIMRKGFKEKLRPSDVYQAPSQDAADILAERLEKEWDREVASGK 65

  Fly    74 KKKKTPSLLKASMNVFGWRLAGLGLVLFILEIGFRVTQPLFLGGLVAYYADASNQEGDNQTKAYL 138
            ||   ||||:|....:.......|.:|:|.| ..:..||..||.::|.:..|...|..|   .|.
Zfish    66 KK---PSLLRAMARCYIKPFLLFGFLLYIGE-ATKTVQPQLLGRIIASFDPAHEPERAN---GYF 123

  Fly   139 YALGVILTSACNVLFMHPYMLGMFHIGMKARIAMTSMIYRKALRLSRTALGDTTIGQVVNLISND 203
            .|.|:.|......|.:.|.|.|:.|:||:.|||:.|:||:|.|:||...|...:.||:|:|:|.:
Zfish   124 LAFGLGLLFTARFLLLQPAMFGLHHLGMQIRIALFSIIYKKTLKLSSRVLDKISTGQLVSLMSAN 188

  Fly   204 VGRLDVSVIHMNYLWLGPVEIGIITYLMYREIGISAFFGVAVMLLFIPLQAYLGKKTSVLRLKTA 268
            :|:.|.|:...:::|:.|::..:.|.|::..|.:::|..:|.:.|...|||:|..|....:.:..
Zfish   189 LGKFDQSLGMAHFIWISPLQCILCTGLIWELIDVNSFCALAAISLLGVLQAFLSHKMGPYKAQKV 253

  Fly   269 LRTDERVRMMNEIISGIQVIKMYAWEIPFSKMINYVRTKEMNAIRNVNYIRGTLQSFIMFVTRIS 333
            |.|::|:.:.:||:..:..:|.|.||.....:|..:|..|:...|.:..:| ...|...|.:.|.
Zfish   254 LLTNKRLALTSEIMENLHSVKAYGWEEIMETLIKNIRQDEVKLTRKIGSLR-YFYSSAYFFSAIF 317

  Fly   334 VFVSLVGFVLLGKLLTAEKAFVITAYYNILRNTMTVYFPMGISQFAELLVSIRRIQTFMLHEETK 398
            |.|:.|....|.:.:...:.|...:|..:||.|:|...|..|..:.:.:..|.:|:.|:..||.|
Zfish   318 VIVAAVVPHALSRGINLRRIFTTLSYCMVLRMTVTRQLPGSIQMWYDTMRLIWKIEEFLSKEEYK 382

  Fly   399 VRDKSEDLDEQKLGKAGLIAEPTVAQTTGVLKPSSRRTSEAEHSIVISKLKAKWD---------- 453
            :.:....:.|.:|                                  ..:.|.||
Zfish   383 LMEYDLSITELEL----------------------------------QDVTASWDEGPGELLERI 413

  Fly   454 -QKNTDN------------------TLDNISLKFKPRQLVAVIGPVGSGKSSLIQAVLGELNPDS 499
             |:|..|                  .|.:||||.|..:::||.|.:|||||||:..:||||.|.|
Zfish   414 KQENKANGHHNGDAGLFFTNLYVAPVLKDISLKLKKGEMLAVTGSMGSGKSSLLMTILGELVPSS 478

  Fly   500 GSVKVNGTLSYASQEPWLFTGTVRQNILFGLPMDKHRYRTVVKRCALERDFELLPYADKTIVGER 564
            |.::.:|.:||:||..|:..||:|.||||||..|::||::|||.|.||.|...||..|||.:.|.
Zfish   479 GKIRHSGRISYSSQTAWIMPGTIRDNILFGLTYDEYRYKSVVKACQLEEDLAALPEKDKTPMAEG 543

  Fly   565 GASLSGGQKARISLARAVYRKADIYLLDDPLSAVDTHVGRHLFDQCMRGFLREEIVLLVTHQLQF 629
            |.:||||||||::|||||||.||:||||.|.:.:|....:.:||:|:...:..:..:|||::::.
Zfish   544 GLNLSGGQKARVALARAVYRDADLYLLDAPFTHLDIATEKEIFDKCLCKLMASKTRILVTNKIEH 608

  Fly   630 LEQADVIVIMDKGKISAMGTYESMAKSGLDFAQM------------------LTDPSKK---DEG 673
            |::||.|:::..|:....||:..:.....||:.:                  ||:...:   ||.
Zfish   609 LKRADKILLLHNGESFFYGTFPELQSERPDFSSLLLGLEAYDNISAERRCSILTETLHRVSVDES 673

  Fly   674 AG-------------------------------------------------DAPDKK-SLSRQN- 687
            ||                                                 ..||:| ||..:|
Zfish   674 AGMQPERSAFRQVPPTKPMYIDERKASVIVNPLGVARKASFIQVPEEEVRRTLPDRKFSLVPENE 738

  Fly   688 --------SKLRDRHG---------SISSMESAAE----------------------SLAAESPM 713
                    |.:...||         |:.:..:.|:                      .||:|..:
Zfish   739 LVDESFMGSDVYHNHGVHMAGQRRQSVLAFMTNAQGQGRREHLQSSFRRRLSVVPQSELASELDI 803

  Fly   714 QT--------------QEGRVEGRIGMKL--------------YKKYFGANGYGLFI-VFAFFCI 749
            .|              :|..:|..:..::              |.:|...|...|:: :|..|..
Zfish   804 YTRRLSDSTYDMTGILEEENIEACLTDEIDEIEETFETTKWNTYVRYVSNNKSLLYVLIFILFIA 868

  Fly   750 GAQVLASGGDIFL---SYWVNKNGEAERDTFMARLRRAFPETRINADT-DPVDIYYFTGINV--- 807
            ..::..|...|||   ..|..::..:|.:  |.:...|....:..|.| .|...||...|.|
Zfish   869 AIEIAGSVAGIFLITDELWREEHQRSEPN--MTKHSNASSSGQTYAITVTPTSSYYILYIYVATS 931

  Fly   808 -SVIIFSLVRSMLFFYLAMRSSTTLHNTMFQGVTRAAMHFFNTNPSGRILNRFSKDLGQVDEILP 871
             |::.....|.:.|.:..:..|..||..|...|..|.|...||..:|||:|||:||:..:|::||
Zfish   932 ESLLAMGFFRGLPFVHTTITISKKLHQKMLHAVLSAPMSVLNTMKTGRIMNRFTKDMATIDDMLP 996

  Fly   872 SVMMDVMQIFLAIVGIVVVLCIINVWYILATVFLVIVFYLLRVFYLSTSRDVKRLEAVTRSPIYS 936
            .:|.|.:|:.:.:||.::|:.|:..:..||...|.|:|.::|.::|.|.:.:|:||...||||:|
Zfish   997 LLMFDFVQLTVVVVGCILVVSIVRPYIFLAATPLAIIFIVMRKYFLRTGQQLKQLETEARSPIFS 1061

  Fly   937 HLSASLNGLATIRAFGAQKELIAEFDNYQDMHSSGYYMFLATSRAFGYWLDCVCVVYIAVITLSF 1001
            ||..||.||.|||||..|....|.|....:.|::.::::|:|.|.|.:..|   ::::...||:.
Zfish  1062 HLIMSLKGLWTIRAFERQAYFEALFHKTLNTHTATWFLYLSTLRWFLFRAD---ILFVFFFTLAA 1123

  Fly  1002 FL---FSPENGGDVGLAITQAMGMTGMVQWGMRQSAELENTMTAVERVVEYEDLEPEGDFESKPN 1063
            ::   .:.:..|::|:.|..||.:.|..||.:..|..::..|.:|:||.::.||..|   ..||:
Zfish  1124 WIAVGTNQDKPGEIGIIICLAMLILGTFQWCVATSIAVDGMMRSVDRVFKFIDLPSE---TPKPD 1185

  Fly  1064 KKPPKD-------------WPEDGKIVFDDLSLKYFPDKAADYVLRSLNIAIEGCEKVGIVGRTG 1115
            |....|             ||..|:|...:|::||  .:|...||::|:.:.||.::|||:||||
Zfish  1186 KGKDSDLIIENVDAQADSSWPHRGQIEVRNLTVKY--TEAGHAVLKNLSFSAEGRQRVGILGRTG 1248

  Fly  1116 AGKSSLINALFRLSYNEGAILIDRRDTNDLGLHDLRSKISIIPQEPVLFSGTMRYNLDPFDEYSD 1180
            :|||||.|||.:|.|.:|.|.||..:.|.:.|...|....::||:..:|:|.:|.||||:..:||
Zfish  1249 SGKSSLFNALLKLVYTDGEISIDGVNWNKMPLQKWRKAFGVVPQKVFIFTGPLRMNLDPYGCHSD 1313

  Fly  1181 AKLWESLEEVKLKQVVADLPSGLQSKISEGGTNFSVGQRQLVCLARAILRENRILVMDEATANVD 1245
            .:||...|||.||.|:...|..|..::..||...|.|.:||:||||:||...|||::||.:|::|
Zfish  1314 EELWRVAEEVGLKTVIEQFPDKLDFQLEYGGYVLSNGHKQLICLARSILSGARILLLDEPSAHLD 1378

  Fly  1246 PQTDALIQTTIRNKFKDCTVLTIAHRLHTVMDSDKVLVMDAGKAVEFGSPFELLTTSEKKVFHSM 1310
            |.|..:::.|:|..|..||:|...|::..:::....|:||.|:...:.|..:||..:      |.
Zfish  1379 PVTIKVLKKTLRQSFSTCTILLSEHKVEPLLECQSFLMMDKGQVKTYDSIQKLLNET------SH 1437

  Fly  1311 VKQTGDSTFDALLKVAQKVGLRFKIQCNLSTCLSSLSSF-----QAHDDNLR 1357
            :||.        :..|:::.|..:...::.|..|.|||.     :..:||::
Zfish  1438 LKQA--------ISPAERLKLFPRRNSSMRTPQSKLSSVTQTLQEEAEDNIQ 1481

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG4562	NP_001262747.1	CFTR_protein	12..1300	CDD:273530	441/1480 (30%)
		ABC_membrane	98..367	CDD:294371	79/268 (29%)
		ABCC_MRP_domain1	443..643	CDD:213217	94/228 (41%)
		ABC_membrane	799..1027	CDD:294371	78/234 (33%)
		ABCC_MRP_domain2	1074..1295	CDD:213211	88/220 (40%)
cftr	NP_001038348.1	AAA	1..1485	CDD:333001	454/1547 (29%)
		Disordered R region. /evidence=ECO:0000250\|UniProtKB:P13569	653..826		22/172 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1452..1485		7/30 (23%)
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P13569	1483..1485
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170586103
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	5	4.850