DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG4562 and Cftr

DIOPT Version :9

Sequence 1:	NP_001262747.1	Gene:	CG4562 / 42362	FlyBaseID:	FBgn0038740	Length:	1408	Species:	Drosophila melanogaster
Sequence 2:	NP_066388.1	Gene:	Cftr / 12638	MGIID:	88388	Length:	1476	Species:	Mus musculus

Alignment Length:	1480	Identity:	416/1480 - (28%)
Similarity:	723/1480 - (48%)	Gaps:	204/1480 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LPENPREKSNIFSSLMFCFAMPTFFKGRKKTLDENDLYRALQEHRSDHLGSKLSAAWEKEVEKKR 73
            :.::|.||::..|.|.|.:..|...||.:..|:.:|:|:|.....:|||..||...|::|    :
Mouse     1 MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDRE----Q 61

  Fly    74 KKKKTPSLLKASMNVFGWRLAGLGLVLFILEIGFRVTQPLFLGGLVAYYADASNQEGDNQTKAYL 138
            ..||.|.|:.|....|.||....|::|::.|: .:..||:.||.::|.| |..|:. :.....||
Mouse    62 ASKKNPQLIHALRRCFFWRFLFYGILLYLGEV-TKAVQPVLLGRIIASY-DPENKV-ERSIAIYL 123

  Fly   139 YALGVILTSACNVLFMHPYMLGMFHIGMKARIAMTSMIYRKALRLSRTALGDTTIGQVVNLISND 203
             .:|:.|......|.:||.:.|:..|||:.|.||.|:||:|.|:||...|...:|||:|:|:||:
Mouse   124 -GIGLCLLFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNN 187

  Fly   204 VGRLDVSVIHMNYLWLGPVEIGIITYLMYREIGISAFFGVAVMLLFIPLQAYLGKKTSVLRLKTA 268
            :.:.|..:...:::|:.|:::.::..|::..:..|||.|:.::::.:..||.|||.....|.:.|
Mouse   188 LNKFDEGLALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRA 252

  Fly   269 LRTDERVRMMNEIISGIQVIKMYAWEIPFSKMINYVRTKEMNAIRNVNYIRGTLQSFIMFVTRIS 333
            .:.:||:.:.:|||..|..:|.|.||....|||..:|..|:...|...|:|....|...|.....
Mouse   253 AKINERLVITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMRFFTSSAFFFSGFFV 317

  Fly   334 VFVSLVGFVLLGKLLTAEKAFVITAYYNILRNTMTVYFPMGISQFAELLVSIRRIQTFMLHEETK 398
            ||:|::.:.::..:: ..|.|...::..:||.::|..||..:..:.:....||:||.|:..:|.|
Mouse   318 VFLSVLPYTVINGIV-LRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFLQKQEYK 381

  Fly   399 VRDKSEDLDEQKLGKAGLIAEPTVA--------------QTTGVLKPSSRRTSEAEHSIVISKLK 449
            |.       |..|...|:|.|...|              |:.|     .|:.|..|:::..|.|.
Mouse   382 VL-------EYNLMTTGIIMENVTAFWEEGFGELLEKVQQSNG-----DRKHSSDENNVSFSHLC 434

  Fly   450 AKWDQKNTDNTLDNISLKFKPRQLVAVIGPVGSGKSSLIQAVLGELNPDSGSVKVNGTLSYASQE 514
            ...:.     .|.||:|..:..:::|:.|..||||:||:..:||||....|.:|.:|.:|:.||.
Mouse   435 LVGNP-----VLKNINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQF 494

  Fly   515 PWLFTGTVRQNILFGLPMDKHRYRTVVKRCALERDFELLPYADKTIVGERGASLSGGQKARISLA 579
            .|:..||:::||:||:..|::||::|||.|.|::|.......|.|::||.|.:|||||:||||||
Mouse   495 SWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLA 559

  Fly   580 RAVYRKADIYLLDDPLSAVDTHVGRHLFDQCMRGFLREEIVLLVTHQLQFLEQADVIVIMDKGKI 644
            ||||:.||:||||.|...:|......:|:.|:...:..:..:|||.:::.|.:||.|:|:.:|..
Mouse   560 RAVYKDADLYLLDSPFGYLDVFTEEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSS 624

  Fly   645 SAMGTYESMAKSGLDF------------------AQMLTDPSKK---DEGAGDAPDKKSLSRQNS 688
            ...||:..:.....||                  :.:||:..::   |:.:......|...||..
Mouse   625 YFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDSSAPWSKPKQSFRQTG 689

  Fly   689 KLRDRH-----GSISSMESAAESLAAESPM-------QTQEGRV-------EGRIGM-------- 726
            ::.::.     .|.||:...  |:..::|:       ..||.|:       :|...:
Mouse   690 EVGEKRKNSILNSFSSVRKI--SIVQKTPLCIDGESDDLQEKRLSLVPDSEQGEAALPRSNMIAT 752

  Fly   727 ----------------------------------------------------------------- 726

Mouse   753 GPTFPGRRRQSVLDLMTFTPNSGSSNLQRTRTSIRKISLVPQISLNEVDVYSRRLSQDSTLNITE 817

  Fly   727 ------------------------KLYKKYFGANGYGLFIVFAFFCIGAQVLASGGDIFLSYWVN 767
                                    ..|.:||..: .||.:|. .:|:...::.....:|: .|:.
Mouse   818 EINEEDLKECFLDDVIKIPPVTTWNTYLRYFTLH-KGLLLVL-IWCVLVFLVEVAASLFV-LWLL 879

  Fly   768 KNGEAERDTFMARLRRAFPETRINADTDPVDIYYFTGINVSVIIFSLVRSMLFFYLAMRSSTTLH 832
            ||..........::..:.....|.:.:.....|.:.|:..:::..||.|.:...:..:.:|..||
Mouse   880 KNNPVNSGNNGTKISNSSYVVIITSTSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILH 944

  Fly   833 NTMFQGVTRAAMHFFNTNPSGRILNRFSKDLGQVDEILPSVMMDVMQIFLAIVGIVVVLCIINVW 897
            ..|...:..|.|...:...:|.||||||||:..:|:.||..:.|.:|:...::|.::|:..:..:
Mouse   945 RKMLHSILHAPMSTISKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPY 1009

  Fly   898 YILATVFLVIVFYLLRVFYLSTSRDVKRLEAVTRSPIYSHLSASLNGLATIRAFGAQKELIAEFD 962
            ..||||..::||.|||.::|.|::.:|:||:..||||::||..||.||.|:|||..|......|.
Mouse  1010 IFLATVPGLVVFILLRAYFLHTAQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFH 1074

  Fly   963 NYQDMHSSGYYMFLATSRAFGYWLDCVCVVYIAVITLSFFLFSPENGGDVGLAITQAMGMTGMVQ 1027
            ...::|::.::|:|||.|.|...:|.:.|::..|:|....|.:.|..|..|:.:|.||.:...:|
Mouse  1075 KALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQ 1139

  Fly  1028 WGMRQSAELENTMTAVERVVEYEDLEPEGDFESKPNKKPPKD------------------WPEDG 1074
            |.:..|.:.::.|.:|.||.::.|::.|....::..|:.|::                  ||..|
Mouse  1140 WAVNSSIDTDSLMRSVSRVFKFIDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGG 1204

  Fly  1075 KIVFDDLSLKYFPDKAADYVLRSLNIAIEGCEKVGIVGRTGAGKSSLINALFRLSYNEGAILIDR 1139
            ::|..||::||..|..|  ||.:::.:|...::||::||||:|||:|::|..|:...:|.|.||.
Mouse  1205 EMVVKDLTVKYMDDGNA--VLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDG 1267

  Fly  1140 RDTNDLGLHDLRSKISIIPQEPVLFSGTMRYNLDPFDEYSDAKLWESLEEVKLKQVVADLPSGLQ 1204
            ...|.:.|.:.|....:|.|:..:||||.|.||||..::.|.::|:..:||.||.|:...|..|.
Mouse  1268 VSWNSVTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLN 1332

  Fly  1205 SKISEGGTNFSVGQRQLVCLARAILRENRILVMDEATANVDPQTDALIQTTIRNKFKDCTVLTIA 1269
            ..:.:||...|.|.:||:||||::|.:.:|:::||.:|::||.|..:|:..::..|..|||:...
Mouse  1333 FTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCE 1397

  Fly  1270 HRLHTVMDSDKVLVMDAGKAVEFGSPFELLTTSEKKVFHSMVKQTGDSTF 1319
            ||:..::|..:.||::.....::.|...||  |||.:|...:..:....|
Mouse  1398 HRIEAMLDCQRFLVIEESNVWQYDSLQALL--SEKSIFQQAISSSEKMRF 1445

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG4562	NP_001262747.1	CFTR_protein	12..1300	CDD:273530	410/1456 (28%)
		ABC_membrane	98..367	CDD:294371	81/268 (30%)
		ABCC_MRP_domain1	443..643	CDD:213217	78/199 (39%)
		ABC_membrane	799..1027	CDD:294371	75/227 (33%)
		ABCC_MRP_domain2	1074..1295	CDD:213211	78/220 (35%)
Cftr	NP_066388.1	CFTR_protein	1..1464	CDD:273530	416/1480 (28%)
		ABC_membrane	85..303	CDD:294371	72/221 (33%)
		ABCC_CFTR1	389..670	CDD:213258	94/290 (32%)
		CFTR_R	639..844	CDD:291094	17/206 (8%)
		Disordered R region. /evidence=ECO:0000250\|UniProtKB:P13569	654..826		15/173 (9%)
		ABC_membrane	861..1142	CDD:279056	83/281 (30%)
		ABCC_CFTR2	1204..1464	CDD:213256	86/246 (35%)
		Interaction with GORASP2. /evidence=ECO:0000250\|UniProtKB:P13569	1382..1476		17/66 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1446..1476		416/1480 (28%)
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P13569	1474..1476
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167841710
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.850