DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG4562 and CFTR

DIOPT Version :9

Sequence 1:	NP_001262747.1	Gene:	CG4562 / 42362	FlyBaseID:	FBgn0038740	Length:	1408	Species:	Drosophila melanogaster
Sequence 2:	NP_000483.3	Gene:	CFTR / 1080	HGNCID:	1884	Length:	1480	Species:	Homo sapiens

Alignment Length:	1476	Identity:	419/1476 - (28%)
Similarity:	733/1476 - (49%)	Gaps:	208/1476 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LPENPREKSNIFSSLMFCFAMPTFFKGRKKTLDENDLYRALQEHRSDHLGSKLSAAWEKEVEKKR 73
            :..:|.||:::.|.|.|.:..|...||.::.|:.:|:|:......:|:|..||...|::|:
Human     1 MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDREL---- 61

  Fly    74 KKKKTPSLLKASMNVFGWRLAGLGLVLFILEIGFRVTQPLFLGGLVAYYADASNQEGDNQTKAYL 138
            ..||.|.|:.|....|.||....|:.|::.|: .:..|||.||.::|.| |..|:| :.....||
Human    62 ASKKNPKLINALRRCFFWRFMFYGIFLYLGEV-TKAVQPLLLGRIIASY-DPDNKE-ERSIAIYL 123

  Fly   139 YALGVILTSACNVLFMHPYMLGMFHIGMKARIAMTSMIYRKALRLSRTALGDTTIGQVVNLISND 203
             .:|:.|......|.:||.:.|:.||||:.||||.|:||:|.|:||...|...:|||:|:|:||:
Human   124 -GIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNN 187

  Fly   204 VGRLDVSVIHMNYLWLGPVEIGIITYLMYREIGISAFFGVAVMLLFIPLQAYLGKKTSVLRLKTA 268
            :.:.|..:...:::|:.|:::.::..|::..:..|||.|:..:::....||.||:.....|.:.|
Human   188 LNKFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFLIVLALFQAGLGRMMMKYRDQRA 252

  Fly   269 LRTDERVRMMNEIISGIQVIKMYAWEIPFSKMINYVRTKEMNAIRNVNYIRGTLQSFIMFVTRIS 333
            .:..||:.:.:|:|..||.:|.|.||....|||..:|..|:...|...|:|....|...|.....
Human   253 GKISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKAAYVRYFNSSAFFFSGFFV 317

  Fly   334 VFVSLVGFVLLGKLLTAEKAFVITAYYNILRNTMTVYFPMGISQFAELLVSIRRIQTFMLHEETK 398
            ||:|::.:.|: |.:...|.|...::..:||..:|..||..:..:.:.|.:|.:||.|:..:|.|
Human   318 VFLSVLPYALI-KGIILRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYK 381

  Fly   399 VRDKSEDLDEQKLGKAGLIAEPTVA---QTTGVL------KPSSRRTSEAEHSIVISKLKAKWDQ 454
            ..       |..|....::.|...|   :..|.|      ..::|:||..:.|:..|........
Human   382 TL-------EYNLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTP 439

  Fly   455 KNTDNTLDNISLKFKPRQLVAVIGPVGSGKSSLIQAVLGELNPDSGSVKVNGTLSYASQEPWLFT 519
                 .|.:|:.|.:..||:||.|..|:||:||:..::|||.|..|.:|.:|.:|:.||..|:..
Human   440 -----VLKDINFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSEGKIKHSGRISFCSQFSWIMP 499

  Fly   520 GTVRQNILFGLPMDKHRYRTVVKRCALERDFELLPYADKTIVGERGASLSGGQKARISLARAVYR 584
            ||:::||:||:..|::|||:|:|.|.||.|.......|..::||.|.:|||||:||||||||||:
Human   500 GTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYK 564

  Fly   585 KADIYLLDDPLSAVDTHVGRHLFDQCMRGFLREEIVLLVTHQLQFLEQADVIVIMDKGKISAMGT 649
            .||:||||.|...:|....:.:|:.|:...:..:..:|||.:::.|::||.|:|:.:|.....||
Human   565 DADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGT 629

  Fly   650 YESMAKSGLDFA------------------QMLTDPSKKDEGAGDAP-----DKKSLSRQNSKL- 690
            :..:.....||:                  .:||:...:....||||     .||...:|..:.
Human   630 FSELQNLQPDFSSKLMGCDSFDQFSAERRNSILTETLHRFSLEGDAPVSWTETKKQSFKQTGEFG 694

  Fly   691 -RDRHGSISSMESAAE-SLAAESPMQ---------------------TQEG-------------- 718
             :.::..::.:.|..: |:..::|:|                     :::|
Human   695 EKRKNSILNPINSIRKFSIVQKTPLQMNGIEEDSDEPLERRLSLVPDSEQGEAILPRISVISTGP 759

  Fly   719 ---------------------------------------------------RVEGRIGMKL---- 728
                                                               |:....|:::
Human   760 TLQARRRQSVLNLMTHSVNQGQNIHRKTTASTRKVSLAPQANLTELDIYSRRLSQETGLEISEEI 824

  Fly   729 ------------------------YKKYFGANGYGLFIVFAFFCIG---AQVLASGGDIFLSYWV 766
                                    |.:|...:...:|::  .:|:.   |:|.||    .:..|:
Human   825 NEEDLKECFFDDMESIPAVTTWNTYLRYITVHKSLIFVL--IWCLVIFLAEVAAS----LVVLWL 883

  Fly   767 NKNGEAERDTFMARLRRAFPETRINADTDPVDIYYFTGINVSVIIFSLVRSMLFFYLAMRSSTTL 831
            ..|...:........|.......|.:.:.....|.:.|:..:::.....|.:...:..:..|..|
Human   884 LGNTPLQDKGNSTHSRNNSYAVIITSTSSYYVFYIYVGVADTLLAMGFFRGLPLVHTLITVSKIL 948

  Fly   832 HNTMFQGVTRAAMHFFNTNPSGRILNRFSKDLGQVDEILPSVMMDVMQIFLAIVGIVVVLCIINV 896
            |:.|...|.:|.|...||..:|.||||||||:..:|::||..:.|.:|:.|.::|.:.|:.::..
Human   949 HHKMLHSVLQAPMSTLNTLKAGGILNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQP 1013

  Fly   897 WYILATVFLVIVFYLLRVFYLSTSRDVKRLEAVTRSPIYSHLSASLNGLATIRAFGAQKELIAEF 961
            :..:|||.:::.|.:||.::|.||:.:|:||:..||||::||..||.||.|:||||.|......|
Human  1014 YIFVATVPVIVAFIMLRAYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLF 1078

  Fly   962 DNYQDMHSSGYYMFLATSRAFGYWLDCVCVVYIAVITLSFFLFSPENGGDVGLAITQAMGMTGMV 1026
            ....::|::.::::|:|.|.|...::.:.|::...:|....|.:.|..|.||:.:|.||.:...:
Human  1079 HKALNLHTANWFLYLSTLRWFQMRIEMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTL 1143

  Fly  1027 QWGMRQSAELENTMTAVERVVEYEDLEPEGDFESKPNK--KPPKD-------------------W 1070
            ||.:..|.::::.|.:|.||.::.|:..||    ||.|  ||.|:                   |
Human  1144 QWAVNSSIDVDSLMRSVSRVFKFIDMPTEG----KPTKSTKPYKNGQLSKVMIIENSHVKKDDIW 1204

  Fly  1071 PEDGKIVFDDLSLKYFPDKAADYVLRSLNIAIEGCEKVGIVGRTGAGKSSLINALFRLSYNEGAI 1135
            |..|::...||:.||  .:..:.:|.:::.:|...::||::||||:|||:|::|..||...||.|
Human  1205 PSGGQMTVKDLTAKY--TEGGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTEGEI 1267

  Fly  1136 LIDRRDTNDLGLHDLRSKISIIPQEPVLFSGTMRYNLDPFDEYSDAKLWESLEEVKLKQVVADLP 1200
            .||....:.:.|...|....:|||:..:||||.|.||||::::||.::|:..:||.|:.|:...|
Human  1268 QIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFP 1332

  Fly  1201 SGLQSKISEGGTNFSVGQRQLVCLARAILRENRILVMDEATANVDPQTDALIQTTIRNKFKDCTV 1265
            ..|...:.:||...|.|.:||:||||::|.:.:||::||.:|::||.|..:|:.|::..|.||||
Human  1333 GKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTV 1397

  Fly  1266 LTIAHRLHTVMDSDKVLVMDAGKAVEFGSPFELLTTSEKKVFHSMV 1311
            :...||:..:::..:.||::..|..::.|..:||  :|:.:|...:
Human  1398 ILCEHRIEAMLECQQFLVIEENKVRQYDSIQKLL--NERSLFRQAI 1441

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG4562	NP_001262747.1	CFTR_protein	12..1300	CDD:273530	416/1460 (28%)
		ABC_membrane	98..367	CDD:294371	86/268 (32%)
		ABCC_MRP_domain1	443..643	CDD:213217	78/199 (39%)
		ABC_membrane	799..1027	CDD:294371	73/227 (32%)
		ABCC_MRP_domain2	1074..1295	CDD:213211	79/220 (36%)
CFTR	NP_000483.3	CFTR_protein	1..1480	CDD:273530	419/1476 (28%)
		ABC_membrane	85..303	CDD:279056	75/221 (34%)
		ABCC_CFTR1	389..670	CDD:213258	93/285 (33%)
		CFTR_R	639..849	CDD:291094	18/209 (9%)
		Disordered R region. /evidence=ECO:0000269\|PubMed:10792060	654..831		16/176 (9%)
		ABC_membrane	866..1147	CDD:279056	84/284 (30%)
		ABCC_CFTR2	1208..1480	CDD:213256	84/238 (35%)
		Interaction with GORASP2. /evidence=ECO:0000269\|PubMed:21884936	1386..1480		16/58 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1452..1480
		PDZ-binding. /evidence=ECO:0000269\|PubMed:11707463, ECO:0000269\|PubMed:16331976	1478..1480
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165151592
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	6	5.850