Protein Alignment Cyp12a5 and cyp27a1.4

DIOPT Version :9

• Sequence 1: NP_650782.1
• Gene: Cyp12a5 / 42293
• FlyBaseID: FBgn0038680
• Length: 536
• Species: Drosophila melanogaster
• Sequence 2: NP_001315442.1
• Gene: cyp27a1.4 / 322341
• ZFINID: ZDB-GENE-030131-1060
• Length: 522
• Species: Danio rerio
• Alignment Length: 539
• Identity: 142/539 - (26%)
• Similarity: 251/539 - (46%)
• Gaps: 61/539 - (11%)

  Fly    15 KPIVFSASQQRWQTNVPTAEIRNDPEWLQAKPFEEIPKANILSLFAKSALPGGKYKN----LEMM 75
            :|...:...:|...|...|.:.......:.|...::|:..|.::..:....|  |.|    |::.
Zfish    19 RPTTAATGFRRAAGNSAAASVSVQDGHRKLKTEADLPEIKIFTMLYQMLFKG--YLNSVHELQLY 81

  Fly    76 EMIDALRQDYGNI--IFLPGMMGRDGLVMTHNPKDFEVVFRNEGVWPFRPGSDILRYHRTVYRKD 138
            :     :|.||.:  |....:.|    :...:.:..|.:.|.:..:|.|....:...||.:
Zfish    82 Q-----KQVYGPLWKINAGNLQG----ISITSVELLEELLRKDEKYPCRGYMTLWTEHRDL---- 133

  Fly   139 FFDGVQ-GIIPSQGKSWGDFRSIVNPVLMQPKNVRLYFKKMSQVNQEFVELIKEIRDAS-TQEVP 201
              .|:. |....:|:.|...|:::|..::.||:...|...::.|..:|::.|..:|:.| |.::.
Zfish   134 --RGISYGPFTEEGEKWYKLRAVLNKRMLHPKDSLQYGDVVNAVITDFIKRIYYLREMSPTGDLV 196

  Fly   202 GNFLETINRWTLESVSVVALDKQLGLLRESGKNSEATKLFKYLDEFFLHSADLEMKPSLWRYFKT 266
            .|....:.|::||.::.:..:.::|.| |....:|..:....:.:.|.::..:.:.|: |.....
Zfish   197 SNLTNELYRFSLEGIASILFETRIGCL-EKEIPAETQEFINSIAQMFTYNMHVALLPN-WTRNYL 259

  Fly   267 PLLKKMLRTMDSVQEVTLKYVDEAIERLEKEAKEGVVRPEHEQSVLEKLL-------KVDKKVAT 324
            |..:|.:...|.:.:...|.::..:|.::       .|.:..|.|..:.|       |:..|...
Zfish   260 PFWQKYIDGWDGIFKFGTKMINLKMEAIQ-------TRLDTNQEVAGEYLTYLLSSGKMSCKDVY 317

  Fly   325 VMAMDMLMAGVDTTSSTFTALLLCLAKNPEKQARLREEVMKVLPNKDSEFTEASMKNVPYLRACI 389
            ....::|:|||||||:|....|..|:|:|..|..|.:||.|||.. |...|...:.::|:|:|.|
Zfish   318 GSVSEVLLAGVDTTSNTMLWALYLLSKDPAAQETLHQEVTKVLKG-DRIPTAEEVNSMPFLKAVI 381

  Fly   390 KESQRVYPLVIGNARGLTRDSVISG-YRVPAGTIVSMIPINSLYSEEYFPKPTEFLPERWLRNAS 453
            ||:.|:||:|..|:|.:....||.| |..|..|..::......:.|:.||:|.:|.||||||
Zfish   382 KETLRLYPVVPVNSRLIAESEVIIGEYLFPKKTTFNLFHYAISHDEKVFPEPQKFKPERWLR--- 443

  Fly   454 DSAGKCPANDLKTK-NPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRNFNVEFNHS---TKNA 514
                     |.:|: |||..:|||||.|.|||:||.|:|:.|..||||:.|.:..:.:   .|..
Zfish   444 ---------DGRTRPNPFGSIPFGFGVRACVGRRIAELEMHLALARLIKLFEMRPDPTVGEVKAN 499

  Fly   515 FRSALINLPNIPLKFKFTD 533
            |||.|:  ||..:...|.:
Zfish   500 FRSVLV--PNKKVNLHFVE 516

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cyp12a5	NP_650782.1	p450	81..531	CDD:278495	130/465 (28%)
cyp27a1.4	NP_001315442.1	p450	66..505	CDD:278495	131/477 (27%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	217	1.000	Domainoid score	I2609
eggNOG	1	0.900	-	-		E2759_KOG0159
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	219	1.000	Inparanoid score	I3547
OMA	1	1.010	-	-		QHG48087
OrthoDB	1	1.010	-	-		D185685at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm6352
orthoMCL	1	0.900	-	-		OOG6_100895
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X156
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	9	8.780