DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Cyp12a5 and CYP27A1

DIOPT Version :9

Sequence 1:	NP_650782.1	Gene:	Cyp12a5 / 42293	FlyBaseID:	FBgn0038680	Length:	536	Species:	Drosophila melanogaster
Sequence 2:	NP_000775.1	Gene:	CYP27A1 / 1593	HGNCID:	2605	Length:	531	Species:	Homo sapiens

Alignment Length:	520	Identity:	146/520 - (28%)
Similarity:	239/520 - (45%)	Gaps:	83/520 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    45 KPFEEIPKANILSLFAKSALPGGKYKNLEMMEMIDALRQDYGNIIFLPGMMGRDGLVMTHNPKD- 108
            :..||||:...|..|.:..:.|  |. |::.::....:..||     |..|...|..|..|...
Human    55 RSLEEIPRLGQLRFFFQLFVQG--YA-LQLHQLQVLYKAKYG-----PMWMSYLGPQMHVNLASA 111

  Fly   109 --FEVVFRNEGVWPFRPGSDILRYHRTVYRKDFFDGVQGIIPSQGKSWGDFRSIVNPVLMQPKNV 171
              .|.|.|.||.:|.|...::.:.||     |..|...|...::|..|...|..:|..|::|...
Human   112 PLLEQVMRQEGKYPVRNDMELWKEHR-----DQHDLTYGPFTTEGHHWYQLRQALNQRLLKPAEA 171

  Fly   172 RLYFKKMSQVNQEFVELIKEIR--DASTQEVPGNFLETINRWTLESVSVVALDKQLGLLRESGKN 234
            .||....::|..:|:..:.::|  .||..:| .:..:....:.||::..:..:|::|.|:.|...
Human   172 ALYTDAFNEVIDDFMTRLDQLRAESASGNQV-SDMAQLFYYFALEAICYILFEKRIGCLQRSIPE 235

  Fly   235 SEATKLFKYLDEFFLHSADLEMKPSLWRYF-------KTPLLKKMLRTMDSVQEVTLKYVDEAIE 292
            ...|         |:.|..|..:.||:..|       ..|..|:.|...:::.....|.:||.:|
Human   236 DTVT---------FVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLE 291

  Fly   293 RLEKEAK----EGVVRPEHEQSVLEKLL---KVDKKVATVMAMDMLMAGVDTTSSTFTALLLCLA 350
            .:|.:.:    :|:    .....|..||   ::..:.|.....::|||||||||:|.|..|..|:
Human   292 DMEAQLQAAGPDGI----QVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLS 352

  Fly   351 KNPEKQARLREEVMKVLP------NKDSEFTEASMKNVPYLRACIKESQRVYPLVIGNARGLTRD 409
            |:||.|..|.|||:.|:|      :||       ..::|.|:|.:||:.|:||:|..|:|.:.::
Human   353 KDPEIQEALHEEVVGVVPAGQVPQHKD-------FAHMPLLKAVLKETLRLYPVVPTNSRIIEKE 410

  Fly   410 SVISGYRVPAGT-------IVSMIPINSLYSEEYFPKPTEFLPERWLRNASDSAGKCPANDLKTK 467
            ..:.|:..|..|       :||..|       ..|.:|..|.|.|||||:.      ||.. :.:
Human   411 IEVDGFLFPKNTQFVFCHYVVSRDP-------TAFSEPESFQPHRWLRNSQ------PATP-RIQ 461

  Fly   468 NPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRNFNVEFNHST---KNAFRSALINLPNIPLKF 529
            :||..:|||:|.|.|:|:||.|:|::|..||||:.:.|.....|   |:..|..|:....:.|:|
Human   462 HPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQF 526

Fly 530 529
Human 527 526

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Cyp12a5	NP_650782.1	p450	81..531	CDD:278495	137/484 (28%)
CYP27A1	NP_000775.1	p450	61..526	CDD:306555	142/512 (28%)
CYP27A1	NP_000775.1	Sterol-binding. /evidence=ECO:0000255	384..398		6/13 (46%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	194	1.000	Domainoid score	I3171
eggNOG	1	0.900	-	-		E2759_KOG0159
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	201	1.000	Inparanoid score	I3768
Isobase	1	0.950	-	0	Normalized mean entropy	S4770
OMA	1	1.010	-	-		QHG48087
OrthoDB	1	1.010	-	-		D185685at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8467
orthoMCL	1	0.900	-	-		OOG6_100895
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X156
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	10	9.730