DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mekk1 and SSK2

DIOPT Version :9

Sequence 1:	NP_732373.1	Gene:	Mekk1 / 42253	FlyBaseID:	FBgn0024329	Length:	1571	Species:	Drosophila melanogaster
Sequence 2:	NP_014428.1	Gene:	SSK2 / 855765	SGDID:	S000005314	Length:	1579	Species:	Saccharomyces cerevisiae

Alignment Length:	1824	Identity:	354/1824 - (19%)
Similarity:	612/1824 - (33%)	Gaps:	665/1824 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 QQSL-RLQKTP------AATTNAEEQVAREEENGNGHHSTVTAETPPTPPIPPIPPIRLRREQSV 73
            |||| :|...|      ...:..|.::.::....|.:...:.  |.||..|.|
Yeast   129 QQSLPKLSTQPVPVHKKVEASKTESEIIKKPAPVNSNQDPLL--TTPTLVISP------------ 179

  Fly    74 EEDVARV---EYRVKQTPSRPVQMTRNRIGALEEDMPPEDELAAHYEAFGTTPPRTRLKIKNRDW 135
              ::|.:   ...:..||......|.|:            .:....:..|:|...|
Yeast   180 --ELASLNTTNTSIMSTPQNITNQTSNK------------HIPTRSQPNGSTSSST--------- 221

  Fly   136 ERKQKVVNVTASADLPASVGGTPKKTRTARS----------RVLRRNTMD-----CALLNEMFVN 185
              .|.:|...:|.......||:....||.:.          |.:|....|     ..:.:..|.:
Yeast   222 --LQDIVTTNSSQRSVGHHGGSTTSLRTYKKQYVLNEQLYLRKMRNRANDDYYTRGIVASSNFED 284

  Fly   186 DESKRTDK-------RLQSLLRDSEREMKN--SLATTAVAAPRGNSFHETAHPLE----SLDQIM 237
            ||...::|       .:..||:....:..|  :.....:.:...|:.:..:..|.    .||.:.
Yeast   285 DEENFSNKGEDDLELEMDDLLKVEGEDKDNDFNFGYNFITSSTKNNENVVSMSLNYLKGKLDWLR 349

  Fly   238 PLNSEAMAPIPLRIASKVVESCNRYRCVSSRPIGYRSSAPPLAFAAQLPAGMLRSDGRATPGLGK 302
            .:|::.    |..|..:...|                    :..:..|.:.:|::     |.:..
Yeast   350 DVNNDQ----PCEIEDEEWHS--------------------ILGSEDLLSKLLQN-----PMVNN 385

  Fly   303 RKDFHETFANLIKLGSVDRQDAKLSQEEH--TWQTELKDLIWLELQAWQADRTVEQQDKYLFEAR 365
            |.::....:.::|...|..:..|::.:..  .:.|:..|.||:||:||...||||.|:|.|...|
Yeast   386 RFEWQTMLSKVLKGDIVRNEKTKIANQGKGPGFNTQFSDDIWIELKAWMNGRTVEDQNKSLRIFR 450

  Fly   366 QGVSDLLTHIINYKFQPRYRREPSLISLDSGTHSDSNSNASSPLPSKMCQGCMSLYCKDCMDHQE 430
            .....:...|:.:|.:                                          |.|...|
Yeast   451 DSTDSVFQEIMAFKLE------------------------------------------DNMSADE 473

  Fly   431 LALREVEGMLTRLEAAEALYPSSQAMGALHPIYKSQSFVGRIKSMCLWYNITKQNKLKLSILGKI 495
             |...::.::.:......|:|:.:.|.|..||.|:::|..||.::..|.|........::.|.|
Yeast   474 -AAETIKSLVDKYYRVLNLWPNIKRMHAEKPITKTEAFRNRIDTLNSWLNFKFNFDTNIAYLKK- 536

  Fly   496 LARLQDEKFSWPVCTSSYIATDSGSSSASGVENDDSAVNSMDSSKPPSMAGSASRKGVTPCHKVQ 560
                      |.|         ......|..|.|::.||..|    |::
Yeast   537 ----------WIV---------GNKELESTTEVDNTTVNLDD----PAV---------------- 562

  Fly   561 FMLNDATHVPGETSSSNESTSTEVSQWSSECSHSHMRKGSMHDINIFSVEPLGTCSSNGTGEQST 625
                                      :::.|
Yeast   563 --------------------------FATNC---------------------------------- 567

  Fly   626 GLYRKFIENVLKSRGLAKSLAFLHKLHNVALYKAHIALEKPGAEDFDYESDAESIEEDVPRLDPE 690
               ::|.|.::|.:.:  .|.|..|:.                               .| |.|.
Yeast   568 ---KRFAEQIMKEKDI--ELIFQKKIF-------------------------------FP-LAPW 595

  Fly   691 ISREQVVELRTYGYWSEEAQSINLPSYIPTFVFLSGIPLQFMHEFLRMRLETRPVRPNP--LSLE 753
            |.:.:...|:....|:|    :||........||...|::.:.:.:.:||.......||  :.::
Yeast   596 ILKAKFFFLKYQKTWNE----LNLSYLDQDLEFLLMFPMRLVKDIILIRLSYAKKIQNPTLMMID 656

  Fly   754 QLMKELREGLTLALTHRERYQRHITTALVENEAELGSY-------ISILNHYDATVRKTFELYLE 811
            |:|.:....:.||:      |...|.|         ||       :.|...:|.||.:..|.:..
Yeast   657 QMMDDFSTYIKLAV------QMKFTVA---------SYCNDWFFKVKIDPEFDHTVVEGLEYFFS 706

  Fly   812 YIDQLVLV----AVPEGNQKSVLEKEW-------------------MFTKLISPMIKGMHT---- 849
            .::..:|.    :.....:..:|.|.|                   .||||...::..:|.
Yeast   707 ILELRILYSGKNSFKTSKEPDLLLKYWEMFRNVGYYIDDAGELIAAEFTKLTLRLVHRLHAYLLR 771

  Fly   850 -----------LASQKFCGIISKLLRSISERL--------------VKRTVE------------- 876
                       .|::|:...|.::|.|:..:|              |:..:|
Yeast   772 QQNTPPKLENEAAAEKWLVQIFEILGSMKRKLNRFTNILTKAFQNFVRYKIEDHNYLLKQLKETG 836

  Fly   877 ---------LDQ---------QIDGTADTD-----DNEEVKWQL---------LTICR------E 903
                     |:|         ::.|..|.|     .|.::...|         |||..      .
Yeast   837 HFLIYTGGYLEQNGTYLIGSPELLGCKDDDILRIIKNSDIGCDLVPKLEINNSLTIYNALDDNWN 901

  Fly   904 TQSLLTVERERSIKVLFFAKTFCRDVETTDFHREHYEHDVANQQHDFICSDVKAAFKLLQQDVLQ 968
            :.|.|..:........::.|   .|:.|   ....|..:..|::.||..|      :..:::..:
Yeast   902 SNSSLGSDISNDGTPFYYIK---NDLTT---QPRSYNGNRVNREPDFENS------RSTEEEFYE 954

  Fly   969 VRNKLTA-------------IIEGVQKRCCLSNMRDLDEQD-KQAVLSRTREILHQGYKFGFEYH 1019
            :..:|.:             :.||....  ||:.:.:..:. ...|:..:.:::.||..:..||.
Yeast   955 LETRLNSLGYVLVLTPQEPLLWEGEMYN--LSDNKTIKPEGLNLKVIPNSIDLMCQGSSYALEYQ 1017

  Fly  1020 KDVIRLFEQKIMD--QKDSGAHTV------------------------------------DLALG 1046
            .|..:......:.  :|.|.:.||                                    ||...
Yeast  1018 CDRFQQISGSSVSFLEKKSSSETVKNNLQRINKAYFRCTYSVLKNYTKIVTTFKKVSPVNDLLNN 1082

  Fly  1047 IIAYAK-----------------------------MWMHFVMERCERGRGMRPRWASQGLEFL-- 1080
            |..:.:                             .|:.|:.|.|:.......||....:||.
Yeast  1083 IFLFGRDFGLNFLRINVANNEKRSIIILLMMRLSIGWLKFLAEDCDPTDQRVFRWCVTSMEFAMH 1147

  Fly  1081 ------ILACDPQITQHLDDDEFEALKQQMDRC----ISH--VIGITS-EPEKVAKKKASPRTRK 1132
                  |||        ||:.:|.:|||::..|    |||  :||..| |.||:.::
Yeast  1148 MVSGWNILA--------LDECQFSSLKQKISECMSLLISHFDIIGARSIEVEKINQQ-------- 1196

  Fly  1133 TSSPATSRSRTPTRTPMSAGMVLNPNTPPLQSPPYNKLLHPQFSLKEDVSGTSYSPVDSSDYVDT 1197
                                                                :.|.:|..|..|.
Yeast  1197 ----------------------------------------------------ARSNLDLEDVFDD 1209

  Fly  1198 PCQRSANGELRLLVPQTPPTPASPGKSSLESTPLALRQERVRDAVNRLDMDLEDGLRERRLIGQV 1262
            ......|.|.|:             :|.:|      .:||::...::....::|..:..:.:
Yeast  1210 DMMLQVNSEFRV-------------QSIME------LEERIKRNPHQTGKVIDDSDKGNKYL--- 1252

  Fly  1263 KSLNSSDKVHIRARSVHFRWHRGIKIGQGRFGKVYTAVNNNTGELMAMKEIAIQPGET--RALKN 1325
            .||.||      ..:|..||.:...||.|.||:||:||:.:.||::|:|||.||..::  :....
Yeast  1253 VSLASS------ISNVSMRWQKRNFIGGGTFGRVYSAVDLDNGEILAVKEINIQDSKSMQKIFPL 1311

  Fly  1326 VAEELKILEGIKHKNLVRYYGIEVHREELLIFMELCSEGTLESLVELTGNLPEALTRRFTAQLLS 1390
            :.||:.:||.:.|.|:|.|||:||||:::.||||.|..|:|.:|:|......|.:|:.:|.|||.
Yeast  1312 IKEEMSVLEILNHPNIVSYYGVEVHRDKVNIFMEYCEGGSLAALLEHGRIEDEMVTQVYTLQLLE 1376

  Fly  1391 GVSELHKHGIVHRDIKTANIFLVDGSNSLKLGDFGSAVKIQAHTT----------VPGE------ 1439
            |::.||:.||||||:|..|| |:|.:..:|..|||:|.||..:.|          ..||
Yeast  1377 GLAYLHESGIVHRDVKPENI-LLDFNGVIKYVDFGAAKKIANNGTRLASMNKIENADGEHEDVTH 1440

  Fly  1440 -------------LQGYVGTQAYMAPEVFTKTNSDGHGRAADIWSVGCVVVEMASGKRPWAQFDS 1491
                         |...:||..|||||..|.:.:.|...|.|:||:||||:||.:|:||||..|:
Yeast  1441 VSDSKAVKNNENALLDMMGTPMYMAPESITGSTTKGKLGADDVWSLGCVVLEMITGRRPWANLDN 1505

  Fly  1492 NFQIMFKVGMGEKPQAP--ESLSQEGHDFIDHCLQHDPKRRLTAVELL------EHNFCKYGRDE 1548
            .:.||:.|..|..||.|  :.:|..|..|::.||..:|.:|.:|||||      :.....:|.|.
Yeast  1506 EWAIMYHVAAGHTPQFPTKDEVSSAGMKFLERCLIQNPSKRASAVELLMDPWIVQIREIAFGDDS 1570

  Fly  1549 CSSE 1552
            .|::
Yeast  1571 SSTD 1574

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mekk1	NP_732373.1	STKc_MEKK4	1281..1542	CDD:270796	117/299 (39%)
Mekk1	NP_732373.1	S_TKc	1282..1541	CDD:214567	116/297 (39%)
SSK2	NP_014428.1	STKc_MEKK4	1265..1558	CDD:270796	117/293 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	200	1.000	Domainoid score	I563
eggNOG	1	0.900	-	-		E1_KOG4645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	282	1.000	Inparanoid score	I552
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002637
OrthoInspector	1	1.000	-	-		otm46482
orthoMCL	1	0.900	-	-		OOG6_104404
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1672
SonicParanoid	1	1.000	-	-		X2354
TreeFam	0	0.000	Not matched by this tool.
	9	8.880