DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mekk1 and Map3k4

DIOPT Version :9

Sequence 1:	NP_732373.1	Gene:	Mekk1 / 42253	FlyBaseID:	FBgn0024329	Length:	1571	Species:	Drosophila melanogaster
Sequence 2:	NP_036078.2	Gene:	Map3k4 / 26407	MGIID:	1346875	Length:	1597	Species:	Mus musculus

Alignment Length:	1738	Identity:	522/1738 - (30%)
Similarity:	782/1738 - (44%)	Gaps:	396/1738 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    51 AETP--------PTPPIPPIPP---IRLRREQSVEEDVARVEYRVKQTPSRPVQMTRNRIGALEE 104
            |:||        |.||..|.|.   ....|::.:..:.||     |...|.|            |
Mouse    15 ADTPAAAMEELRPAPPPQPEPDPECCPAARQECMLGESAR-----KSMESDP------------E 62

  Fly   105 DMPPEDELAAHYEAFGTTPPRTRLKIKNRDWERKQKVVNVTASADLPASVGGTPKKTRT---ARS 166
            |...|......|   ||:||.|..::|....:.::.      ||..|||.....:|..|   :..
Mouse    63 DFSDETNTETLY---GTSPPSTPRQMKRLSAKHQRN------SAGRPASRSNLKEKMNTPSQSPH 118

  Fly   167 RVLRRNTMDCALLNEMFVNDESKRTDKRLQSLLRDSEREMKNSLATTAVAAPRGNSFHETAHPLE 231
            :.|.:....        |.:.|.:.:|::::.||.:||:.|.:.              :.:..|:
Mouse   119 KDLGKGVET--------VEEYSYKQEKKIRATLRTTERDHKKNA--------------QCSFMLD 161

  Fly   232 SLDQIMPLNSEAMAPIPLRIASKVVES--CNRYRCVSSRPIGYRSSAPPLAFAAQLPAGMLRSDG 294
            |:...:|..|     ||....:|...|  |:..:...|.|:       |:|..|:..:   |:|.
Mouse   162 SVAGSLPKKS-----IPDVDLNKPYLSLGCSNAKLPVSMPM-------PIARTARQTS---RTDC 211

  Fly   295 RATPGLGKRKDFHETFANLIKLGSVD-RQDAKLSQEEHT---WQTELKDLIWLELQAWQADRTVE 355
            .|     .|..|.||...|:||.||. ::|.:...:|:|   |.....:|||||||||.|.||:.
Mouse   212 PA-----DRLKFFETLRLLLKLTSVSKKKDREQRGQENTAAFWFNRSNELIWLELQAWHAGRTIN 271

  Fly   356 QQDKYLFEARQGVSDLLTHIINYKFQPRYRREPSLISLDSGTHSDSNSNASSPLPSKMCQ----- 415
            .||.:|:.|||.:.|::..|:.:|           ::..|...|.:.:..:.||....|:
Mouse   272 DQDLFLYTARQAIPDIINEILTFK-----------VNYGSIAFSSNGAGFNGPLVEGQCRTPQET 325

  Fly   416 ---GCMSLYCKDCMDHQELALREVEGMLTRLEAAEALYPSSQAMGALHPIYKSQSFVGRIKSMCL 477
               ||.|.:  :.:..|.::..:|:.::..||..||||||.||:...:..|.::.|..|::::||
Mouse   326 NRVGCSSYH--EHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCL 388

  Fly   478 WYNITKQNKLKLSILGKILARLQDEKFSWPVCTSSYIATDSGSSSASGVENDDSAVNSMDSSKPP 542
            |.||||....||.|:|.:|.........|||..........|......||:.:..:..::|..
Mouse   389 WLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEDEVEDTEVELRELESGT-- 451

  Fly   543 SMAGSASRKGVTPCHKVQFMLNDATHVPGETSSSNESTSTEVSQWSSECSHSHMRKGSMHDINIF 607
                ..|.:..||..:|..:              ..||...:...|..|....:.:....:.:|
Mouse   452 ----EESDEEPTPSPRVPEL--------------RLSTDAILDSRSQGCVSRKLERLESEEDSI- 497

  Fly   608 SVEPLGT--CSSNGTGEQSTGLYRKFIENVLKSRGLAKSLAFLHKLHNVALYKAHIALEKPGA-- 668
               ..||  |....:....|.:||.|::..||..||.|.:..||||.|.:|.:|.:||.|...
Mouse   498 ---GWGTADCGPEASRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMNGSLQRARVALVKDDRPV 559

  Fly   669 --EDFD---YESDAESIEEDVPRLDPEISREQVVELRTYGYWSEEAQSINLPSYIPTFVFLSGIP 728
              .||.   :.||...:....|..:.:.|...         | ||.::::|||:.|.|:.|..:.
Mouse   560 EFSDFPGPMWGSDYVQLSGTPPSSEQKCSAVS---------W-EELRAMDLPSFEPAFLVLCRVL 614

  Fly   729 LQFMHEFLRMRLETRPV-RPNPLSLEQLMKELREGLTLALTHRERYQ---RHITTALVENEAELG 789
            |..:||.|::|||.||. .|:.||::||::|.:|.|...|..::.||   :.:...|.:.:..:.
Mouse   615 LNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLGGLEKTDCNMD 679

  Fly   790 SYISILNHYDATVRKTFELYLEYIDQLV--LVAVPEGNQ--KSVLEKEWMFTKLISPMIKGMHTL 850
            :       ::..::|...:|.:|:...:  |..:|:.:.  |::||:||.|||.|:..|:|....
Mouse   680 A-------FEEDLQKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQ 737

  Fly   851 ASQKFCGIISKLLRSISERLVKRTVELDQQIDGTADTDDN---EEVKWQLLTICRETQSLLTVER 912
            |.:.||.|...||:|....|.....|...::..:|  |||   :|::..::.|.|..:.|....|
Mouse   738 AGKLFCDIAGMLLKSTGSFLESGLQESCAELWTSA--DDNGAADELRRSVIEISRALKELFHEAR 800

  Fly   913 ERSIKVLFFAKTFCRDVE-TTDF-----HREHYEHDVANQQ-----------HDFI--------- 951
            ||:.|.|.|||...:|:| ..:|     .||..:...|.|.           |.|:
Mouse   801 ERASKALGFAKMLRKDLEIAAEFVLSASARELLDALKAKQYVKVQIPGLENLHVFVPDSLAEEKK 865

  Fly   952 -------------CS----DV-------------------------------------------- 955
                         ||    ||
Mouse   866 IILQLLNAATGKDCSKDPDDVFMDAFLLLTKHGDRARDSEDGWGTWEARAVKIVPQVETVDTLRS 930

  Fly   956 -------------------KAAF----------------------KLLQQ---DVLQVRNKLTAI 976
                               :.||                      |.|||   |.|::.|:::..
Mouse   931 MQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVRHEQTSSQPIIAKGLQQLKNDALELCNRISDA 995

  Fly   977 IEGVQKRCCLSNMRDLDEQDKQAVLSRTREILHQGYKFGFEYHKDVIRL----FEQKIMDQKDSG 1037
            |:.|.....|....:::|.:...:....||.:.|||.|||||||:|:||    |.|||.|:
Mouse   996 IDRVDHMFTLEFDAEVEESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDK---- 1056

  Fly  1038 AHTVDLALGIIAYAKMWMHFVMERCERGRGMRPRWASQGLEFLILACDPQITQHLDDDEFEALKQ 1102
                     .|::|:.||::|:.:||.|||.|||||:||.:|| .|.:|.....|.:|:|.:|:.
Mouse  1057 ---------YISFAQKWMNYVLTKCESGRGTRPRWATQGFDFL-QAIEPAFISALPEDDFLSLQA 1111

  Fly  1103 QMDRCISHVIG-----ITS------EPEKVAKKKASP-------------RTRKTSSPA------ 1137
            .|:.||.||||     :|:      .|.||.:..:.|             .||...|.|
Mouse  1112 LMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARTHGNS 1176

  Fly  1138 -------TSRSRTPTRTPMSAGMVLNPNTPPLQSP-------PYNKLLHPQFSLKEDVSGTSY-- 1186
                   .:.:.|....|...|....|..|...:|       |.|..|   .|:..::...|.
Mouse  1177 VAAAAAVAAAATTAAGRPGPGGGDSVPAKPVNTAPDTRGSSVPENDRL---ASIAAELQFRSLSR 1238

  Fly  1187 --SPVDSSDYVDTPCQRSANG-----ELRLLVPQTPPTPASPGK-SSLESTPLALRQERVRDAVN 1243
              ||.:..|....|...|:..     |||.|:.||..:.:..|. .:::.:.....:.|.|:
Mouse  1239 HSSPTEERDEPAYPRSDSSGSTRRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYRE--- 1300

  Fly  1244 RLDMDLEDGLRERRLIGQV-KSLNSSDKV-HIRARSVHFRWHRGIKIGQGRFGKVYTAVNNNTGE 1306
                     :|.:.:|||| .:..|.|.| |:..|.|.|:|.||.|||:|::|||||.::.:|||
Mouse  1301 ---------MRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGE 1356

  Fly  1307 LMAMKEIAIQPGETRALKNVAEELKILEGIKHKNLVRYYGIEVHREELLIFMELCSEGTLESLVE 1371
            |||||||..||.:.:.:|..|:||||.|||||.|||||:|:|:||||:.||||.|.|||||.:..
Mouse  1357 LMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSR 1421

  Fly  1372 LTGNLPEALTRRFTAQLLSGVSELHKHGIVHRDIKTANIFLVDGSNSLKLGDFGSAVKIQAHT-T 1435
            |  .|.|.:.|.:|.|:...::.||:|||||||||.|||||. .|..:||||||.:||::.:. |
Mouse  1422 L--GLQEHVIRLYTKQITVAINVLHEHGIVHRDIKGANIFLT-SSGLIKLGDFGCSVKLKNNAQT 1483

  Fly  1436 VPGELQGYVGTQAYMAPEVFTKTNSDGHGRAADIWSVGCVVVEMASGKRPWAQFDSNFQIMFKVG 1500
            :|||:...:||.|||||||.|:...:||||||||||:||||:||.:|||||.:::.|||||:|||
Mouse  1484 MPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVG 1548

  Fly  1501 MGEKPQAPESLSQEGHDFIDHCLQHDPKRRLTAVELLEHNFCKYGRDE 1548
            ||.||..||.||.||..|:.|||:.|||.|.||.:||:|.|.|...||
Mouse  1549 MGHKPPIPERLSPEGKAFLSHCLESDPKIRWTASQLLDHAFVKVCTDE 1596

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mekk1	NP_732373.1	STKc_MEKK4	1281..1542	CDD:270796	153/261 (59%)
Mekk1	NP_732373.1	S_TKc	1282..1541	CDD:214567	153/259 (59%)
Map3k4	NP_036078.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..129		32/147 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	424..465		7/46 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1137..1157		4/19 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1190..1220		5/29 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1233..1263		6/29 (21%)
		STKc_MEKK4	1331..1590	CDD:270796	153/261 (59%)
		S_TKc	1332..1590	CDD:214567	153/260 (59%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	341	1.000	Domainoid score	I1083
eggNOG	1	0.900	-	-		E1_KOG4645
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H31346
Inparanoid	1	1.050	656	1.000	Inparanoid score	I768
Isobase	1	0.950	-	0	Normalized mean entropy	S4833
OMA	1	1.010	-	-		QHG46651
OrthoDB	1	1.010	-	-		D55144at2759
OrthoFinder	1	1.000	-	-		FOG0002637
OrthoInspector	1	1.000	-	-		oto91948
orthoMCL	1	0.900	-	-		OOG6_104404
Panther	1	1.100	-	-	LDO	PTHR48016
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1672
SonicParanoid	1	1.000	-	-		X2354
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	16	15.820