Protein Alignment htl and EPHA4

DIOPT Version :10

• Sequence 1: NP_524394.2
• Gene: htl / 42160
• FlyBaseID: FBgn0010389
• Length: 729
• Species: Drosophila melanogaster
• Sequence 2: NP_004429.1
• Gene: EPHA4 / 2043
• HGNCID: 3388
• Length: 986
• Species: Homo sapiens
• Alignment Length: 708
• Identity: 175/708 - (24%)
• Similarity: 284/708 - (40%)
• Gaps: 166/708 - (23%)

  Fly    29 SIWQPAVQVEGRRQMANSQEMIKDHLGARSQNKTPAITNNANQSSTSSADLDDGAADDDDNKADL 93
            |:|:.|......|....:..........|..:....:.:|.|:   :|.:|:..:..:...:.|:
Human   299 SVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNE---TSVNLEWSSPQNTGGRQDI 360

  Fly    94 PVNVSSKPYWR-NPKKMSFLQTRPSGSLLTLNCHALGNPEPNITWYRNGTVDWTRGYGSLKRNRW 157
            ..||..|.... :|.|     .||.||                      .|.:|.....||..:.
Human   361 SYNVVCKKCGAGDPSK-----CRPCGS----------------------GVHYTPQQNGLKTTKV 398

  Fly   158 TLTMEDLVPGDCGNYTCKVCNSLGCIRH----DTQVIVSDRVNHKPILMTGPLNLTLVVNSTGSM 218
            ::|  ||:...  |||.::....|..::    |..|.|:...|     ...|.::.||
Human   399 SIT--DLLAHT--NYTFEIWAVNGVSKYNPNPDQSVSVTVTTN-----QAAPSSIALV------- 447

  Fly   219 HCKYLSDLTSKKAWIFVPCHGMTNCSNNRSIIAE------DKDQLDFVNVRMEQEGWYTCV---- 273
            ..|.::..:...||:        .......:|.|      :|||         .|..|..|
Human   448 QAKEVTRYSVALAWL--------EPDRPNGVILEYEVKYYEKDQ---------NERSYRIVRTAA 495

  Fly   274 -ESNSLGQSNSTAYLRVVRS------------LHVLEAGVAS---GSLHSTSFVYIFVFGGLIFI 322
             .::..|.:..|:|:..||:            |.|....|.|   |...:::.:.:.|.|.::.:
Human   496 RNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANSTVLLVSVSGSVVLV 560

  Fly   323 FMTTLFVFYAIRKMKHEKVLKQRIETVHQWTKKVIIFKPEGGGDSSGSMDTMIMPVVRIQKQRTT 387
            .:.......:.|:.|:.|..::..|..|                             ..|..||.
Human   561 VILIAAFVISRRRSKYSKAKQEADEEKH-----------------------------LNQGVRTY 596

  Fly   388 VLQNGNEPAPFNEYEFP----LDSNWELPRSHLVLGATLGEGAFGRVVMAEV-----NNAIVAVK 443
            |       .||. ||.|    .:...|:..|.:.:...:|.|.||.|....:     ....||:|
Human   597 V-------DPFT-YEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIK 653

  Fly   444 MVKEGHTDDDIASLVREMEVMKIIGR--HINIINLLGCCSQNGPLYVIVEYAPHGNLKDFLYKNR 506
            .:|.|:||......:.|..:|   |:  |.|||:|.|..::..|:.:|.||..:|:|..||.||.
Human   654 TLKAGYTDKQRRDFLSEASIM---GQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKND 715

  Fly   507 PFGRDQDRDSSQPPPSPPAHVITEKDLIKFAHQIARGMDYLASRRCIHRDLAARNVLVSDDYVLK 571
              ||                 .|...|:.....|..||.||:....:||||||||:||:.:.|.|
Human   716 --GR-----------------FTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCK 761

  Fly   572 IADFGLARDIQSTDYYRKNT-NGRLPIKWMAPESLQEKFYDSKSDVWSYGILLWEIMTYGQQPYP 635
            ::|||::|.::........| .|::||:|.|||::..:.:.|.||||||||::||:|:||::||
Human   762 VSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPY- 825

  Fly   636 TIMSAEELYTYLMSGQRMEKPAKCSMNIYILMRQCWHFNADDRPPFTEIVEYMDKLLQ 693
            ..||.:::...:..|.|:..|..|.:.::.||..||.....|||.|.:||..:|||::
Human   826 WDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIR 883

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
htl	NP_524394.2	Ig	101..191	CDD:472250	19/94 (20%)
		Ig strand B	121..125	CDD:409353	0/3 (0%)
		Ig strand C	134..138	CDD:409353	0/3 (0%)
		Ig strand E	157..161	CDD:409353	0/3 (0%)
		Ig strand F	171..176	CDD:409353	3/4 (75%)
		Ig strand G	184..187	CDD:409353	0/6 (0%)
		Ig	200..289	CDD:472250	17/99 (17%)
		Ig strand B	216..220	CDD:409353	0/3 (0%)
		Ig strand C	229..233	CDD:409353	1/3 (33%)
		Ig strand E	255..259	CDD:409353	2/3 (67%)
		Ig strand F	269..274	CDD:409353	2/9 (22%)
		Ig strand G	282..285	CDD:409353	0/2 (0%)
		Protein Kinases, catalytic domain	404..692	CDD:473864	101/299 (34%)
EPHA4	NP_004429.1	EphR_LBD_A4	30..203	CDD:198450
		FN3	329..436	CDD:238020	28/140 (20%)
		FN3	441..532	CDD:238020	21/114 (18%)
		EphA2_TM	562..618	CDD:464211	15/92 (16%)
		PTKc_EphR_A	616..882	CDD:270651	99/288 (34%)
		SAM_EPH-A4	911..981	CDD:188944
		PDZ-binding. /evidence=ECO:0000255	984..986