Protein Alignment MAP3K4 and SSK22

DIOPT Version :9

• Sequence 1: NP_005913.3
• Gene: MAP3K4 / 4216
• HGNCID: 6856
• Length: 1608
• Species: Homo sapiens
• Sequence 2: NP_009998.2
• Gene: SSK22 / 850436
• SGDID: S000000669
• Length: 1331
• Species: Saccharomyces cerevisiae
• Alignment Length: 1649
• Identity: 345/1649 - (20%)
• Similarity: 594/1649 - (36%)
• Gaps: 517/1649 - (31%)

Human    92 MKRMSTKHQRNNVGRPASRSNLKEK------MNAPNQPPHKDTGKTVENVEEYSYKQEKK----- 145
            :||:|: |..:.:    |||:||..      .:.|:|...||  :.|.|...|..|.:|.
Yeast    30 VKRLSS-HSSHKL----SRSDLKALGGSETISDGPSQLTFKD--RYVFNESLYLKKLKKTALDDY 87

Human   146 IRAALRTTERDRKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIARPA 210
            ....::.|.|..:.:.....:..|.|..:      |.||:        |..|...:.  |..|..
Yeast    88 YTRGIKLTNRYEEDDGDDEIIRLSNGDRI------DEDLH--------SGVKFFSTT--PYCRKM 136

Human   211 RQTSR----TDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSGFWLNRSNELIWLELQAW 271
            |..|.    .:...:|.|:...|..:||...|..:|.| ...|....|.....|:| |||||:||
Yeast   137 RSDSDELAWNEIATERFKWQSMLARVLKGDIVKGEKTR-IANQVKKPGLNKELSDE-IWLELKAW 199

Human   272 HAGRTINDQDFFLYTARQAIPDIINEILTFKVDYGSFAFVRDRAGFNGTSVEGQCKATPGTKIVG 336
            ..|||:.:.:..|...|.:...:..||:.|::..|                          ||:.
Yeast   200 LNGRTMQEMEQSLTYLRDSSDSVFEEIMKFQIPQG--------------------------KILS 238

Human   337 YSTHHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITK 401
            .......||      :.:.|...::.|    :|:|:.:.||.......:|..|:..:..|||...
Yeast   239 LDALEAILQ------DLMNRYHSVVSY----WPNLKKMYKDKPITNTAEFTARIDVMNSWLNFKT 293

Human   402 DLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEYEGDDTEGELKELESSTDESEEEQI 466
            :|..:                                  ..|.||.......: ||:|..:|:
Yeast   294 NLTLR----------------------------------RQELDDWINRFSPI-SSSDNCQED-- 321

Human   467 SDPRVPEIRQPIDNSFDIQSRDCISKKLERLESEDDSLGWGAPDWSTEAGFSRHCLTSIYRPFVD 531
                           ||                       |.|.|        :|...|   ..:
Yeast   322 ---------------FD-----------------------GVPQW--------NCKMKI---LAE 337

Human   532 KALKQMGLRKLI--------------LRLHKLMDGSLQRARIALVKNDRPVEFSEFPDPMWGSDY 582
            :.:|:..:..:.              |:||.::      .|..|.|.:     .::|
Yeast   338 QLMKEKNIESIFQKKIFYPLSPWMFKLKLHFIV------YRETLTKMN-----IKYP-------- 383

Human   583 VQLSRTPPSSEEKCSAVSWEELKAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLS 647
                              :|.|::  |.:| |.:|:. .|:|..:....||:       .|:::.
Yeast   384 ------------------YERLRS--LLAF-PVYLIK-EVILTRLSYARKLK-------NPTMMM 419

Human   648 IKQLVRECKEVLKGGLLMKQYYQFMLQEVLEDLEKP-DCNID-AFEEDLHKMLMVYFDYMRSWIQ 710
            |.|::.:....::..:.:|    :.|.:...:|  | |.:.| .||..:.:.:...|     ::.
Yeast   420 IDQMIDDFNAFIRLSVQLK----YTLTKYCSNL--PFDVDFDPTFENTVIEAIRYLF-----FLL 473

Human   711 MLQQLPQASHSLK--NLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQES 773
            .|:.:..:..:.|  :||.:.|:..|...|||.|.|......|..:...|:..    |:|.|.:.
Yeast   474 NLKLIDSSKQNFKAPDLLLKYWDHLKNTGHYINGAETVIPNEFLKLTLRLVHK----LQFYLLKQ 534

Human   774 CAEFWTSADDSSASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFRLSAPV 838
             ..|..:..::|.:::...|:.|...|:|.          |...|:.:|.|..:.:|.::::...
Yeast   535 -QNFPPTFANASEAEKWLSSIFENLGAMKR----------KLNRFSNILVKAFQNSAVYQINHNA 588

Human   839 RDLLDVLKSKQYVKVQIPGL---ENLQMF-VPDTLAEEKSIILQLL-NAAAGKD----------- 887
            : |:..||...|..|.....   ..:.|| .|:.|..:...||::| |.:.|.|
Yeast   589 Q-LVKKLKDAHYFLVYSGNTFESSGVYMFAAPELLGCDNDTILRILRNKSIGCDLVPKLDIGNNL 652

Human   888 ---------------CSKDSDDVLIDAYLL-------LTKHGDRARDSE---------------- 914
                           .||..|...|..|.:       |.:|..:::...
Yeast   653 NVYDITTKETDLNILVSKGEDSKGIPYYRVVANSSSDLDRHAHQSKKKNFSTDPFDQHLDEKNNE 717

Human   915 --------DSWG-----------TWEAQPVKVVPQVETVDTLRSMQVD-------NLLLVVMQSA 953
                    .|.|           .|:. ||..:|   ..:...|.::|       |.|:::.|.:
Yeast   718 VFELEVALSSLGALVVLYPGEPVVWDG-PVYKLP---GNNLFASNEMDLGKIGNPNTLILLNQGS 778

Human   954 H--LTIQRKAFQQSIEGLMTLCQEQTSSQPVIAKALQQLKNDALELCNRISNAIDRVDHMFTSEF 1016
            :  ||.|...|.|::...::..:::.|... |..:||::.....:|...:.|....:...|..:.
Yeast   779 NYALTYQIDKFNQTVGDSVSFIEKRCSLNS-IESSLQKINKAYYKLTYTVLNNYKGILGSFMKQC 842

Human  1017 DAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKC 1081
            ..          .:......:.|.:||..:.|  ....|.:.:..|....:.....|:.:::.:|
Yeast   843 PG----------NELLNSIFMFGRDFGRSFLK--YNAFSSKRKYVIIFLMVKLGMNWLKFLVEEC 895

Human  1082 ESGRGTRPRWATQGFDFLQAIEPAF-ISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIH 1145
            :.......||.....||...:...: |.||....|..|:..::.|:..:|
Yeast   896 DPTDQRTFRWCVLAMDFAMQMTSGYNILALNVKQFQELKERVSVCMSLLI--------------- 945

Human  1146 RNSPRPMKVPRCHSDPPNPHLIIPTPEGFSTRSMPSDARSHGSPAAAAAAAAAAVAASRPSPSGG 1210
                                                   ||.....|.|..|
Yeast   946 ---------------------------------------SHFDVMGARATEA------------- 958

Human  1211 DSVLPKSISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEER-DEPAYPRGDSSGSTRRS 1274
                                ||.           ...:|.:..|||. ||.|
Yeast   959 --------------------ENG-----------MQQARLNIDTEENIDEEA------------- 979

Human  1275 WELRTLISQSKDTASKLGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKV 1339
                ||...|:   .:|..|:.::|:::       |..|:...:....|....|...:...|..|
Yeast   980 ----TLEINSR---LRLEAIKTLEKTMK-------RNPRQMGKVLDATDQGNKYLLSLASSLSNV 1030

Human  1340 TFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKE----TADELKIFEGIKHP 1400
            :.:||:.:.||.|.:|:||:.|:::.||::|:|||:.  :|..|:|:    ..:|:.:.|.:.||
Yeast  1031 SMRWQKRSFIGGGTFGQVYSAINLENGEILAVKEIKI--HDTTTMKKIFPLIKEEMTVLEMLNHP 1093

Human  1401 NLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGL--QEHVIRLYSKQITIAINVLHEHGIVHRD 1463
            |:|:|:|||:||:::.||||||:.|:|..:...|.  .|.|.::|:.::...:..||:.|:||||
Yeast  1094 NIVQYYGVEVHRDKVNIFMEYCEGGSLASLLDHGRIEDEMVTQVYTFELLEGLAYLHQSGVVHRD 1158

Human  1464 IKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPG-------------------EVNSTLGTAAYM 1509
            ||..||.|..:|:||..|||       .|:|:.|                   .:|..:||..||
Yeast  1159 IKPENILLDFNGIIKYVDFG-------TARTVVGSRTRTVRNAAVQDFGVETKSLNEMMGTPMYM 1216

Human  1510 APEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPER--LSP 1572
            |||.|:.:..:|...|.|:|:|||||:||.||:|||...::.:.|||.|..|..|.:|.|  ::.
Yeast  1217 APETISGSAVKGKLGADDVWALGCVVLEMATGRRPWSNLDNEWAIMYHVAAGRIPQLPNRDEMTA 1281

Human  1573 EGKDFLSHCLESDPKMRWTASQLL 1596
            .|:.||..||..||.||.||.:||
Yeast  1282 AGRAFLERCLVQDPTMRATAVELL 1305

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
MAP3K4	NP_005913.3	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..136		15/49 (31%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	429..478		7/48 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1157..1190		2/32 (6%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1202..1231		0/28 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1244..1274		7/30 (23%)
		STKc_MEKK4	1342..1601	CDD:270796	109/282 (39%)
SSK22	NP_009998.2	STKc_MEKK4	1033..1309	CDD:270796	109/282 (39%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	200	1.000	Domainoid score	I639
eggNOG	1	0.900	-	-		E1_KOG4645
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	248	1.000	Inparanoid score	I756
Isobase	1	0.950	-	0	Normalized mean entropy	S4833
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002637
OrthoInspector	1	1.000	-	-		otm53244
orthoMCL	1	0.900	-	-		OOG6_104404
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1672
SonicParanoid	1	1.000	-	-		X2354
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	11	10.740