DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Brf and brf1b

DIOPT Version :9

Sequence 1:	NP_001163636.1	Gene:	Brf / 42087	FlyBaseID:	FBgn0038499	Length:	662	Species:	Drosophila melanogaster
Sequence 2:	NP_956183.1	Gene:	brf1b / 334316	ZFINID:	ZDB-GENE-030131-6248	Length:	693	Species:	Danio rerio

Alignment Length:	723	Identity:	302/723 - (41%)
Similarity:	432/723 - (59%)	Gaps:	103/723 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 KCRNCGSNEIEEDNARGDRVCMNCGSVLEDSLIVSEVQF-EEVGHGAAAIGQFVSAESSGGATNY 69
            :|:|||.::|:.|.|||..||..|||||||::||||||| |..|..::|:||||||:.:..|...
Zfish     4 QCKNCGGSDIDTDPARGSAVCTACGSVLEDNIIVSEVQFVENSGGVSSAVGQFVSADGTSKAPVL 68

  Fly    70 GYGKFQVGSGTESREVTIKKAKKDITLLCQQLQLSQHYADTALNFFKMALGRHLTRGRKSTHIYA 134
            |.| |....|.|||..|::..|:.|..|..||||:||..|||.|||||.:.:|||||||.||:.|
Zfish    69 GSG-FHTSLGKESRAQTLQNGKRQIHNLGSQLQLNQHCLDTAFNFFKMVVSKHLTRGRKMTHVIA 132

  Fly   135 ACVYMTCRTEGTSHLLIDISDVQQICSYELGRTYLKLSHALCINIPSLDPCLYIMRFANRLQLGA 199
            ||:|:.||||||.|:|:|:||:.|:..|.||:|:|.|:..||||.|::||||||.|||:.|:.|.
Zfish   133 ACLYLVCRTEGTPHMLLDLSDLLQVNVYILGKTFLLLARELCINAPAVDPCLYIPRFAHMLEFGE 197

  Fly   200 KTHEVSMTALRIVQRMKKDCMHSGRRPTGLCGAALLIAARMHDFSRTMLDVIGVVKIHESTLRKR 264
            |||||||||||::||||:|.||:||||:||||||||:|||||:|.||:.:||.|||:.|:|||||
Zfish   198 KTHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHEFRRTVKEVISVVKVCETTLRKR 262

  Fly   265 LSEFAETPSGGLTLEEFMTVDLEREQDPPSFKAARKK------DRERIKDMGEHELTELQKEIDA 323
            |:||.|||:..||::|||.||||:|.|||||.|.:||      ::|.:|     :|.|.|.|||:
Zfish   263 LTEFEETPTSSLTIDEFMRVDLEKECDPPSFVAGQKKVKMQQLEQELVK-----KLEEYQGEIDS 322

  Fly   324 HLEKDLGKYSNSVYRQL-----TKGKGL-------SPLSSPSTPNSSSEKDIELEESRQFIEQSN 376
                    |.:.:..||     ||.||:       ...|...|...:.::|.|.||.....:..|
Zfish   323 --------YRDEIETQLAGSSRTKMKGIYASYAREDDDSLSVTSGLTGDEDAEDEEMEAAAKHLN 379

  Fly   377 AEVIKELIAKNEDVKKSEPGGL--------------VAGIEGLRPDIEAICRVTQSDLEDVEKAK 427
            .|.|.:::.:.:...|.:..|.              :..:.|..|...:: .:|.|..:.:.:..
Zfish   380 QEFISQVLNRRDQGGKKDLDGRKNTVPLPNQPKHVPLTALLGPLPSAASL-GLTDSIRQCITETT 443

  Fly   428 QPQEQ----ELITDDLNDDELDQYVLTEEESVAKLEMWKNLNAEYLQEQKERDERLAKEREEGKP 488
            ..:|:    ||..|.::::|:::|:|.|.|..||.|:|...|.|||:||||::||:|||:|:|..
Zfish   444 NVEEKSDSGELDLDGIDEEEIEKYILNEIEVEAKTELWMKQNEEYLREQKEKEERIAKEKEQGTY 508

  Fly   489 ERKKRKPRKK---VIGPSSTAGEAIEKMLQEKKISSKINYEILKTLTDGMGGLT------DDSPT 544
            :.|.:||.||   ::  :|||||||||||::|||||||||::|:.| :..|..:      :|.||
Zfish   509 KEKPKKPSKKREPIL--ASTAGEAIEKMLEQKKISSKINYDVLRDL-NSKGSASSSTQQAEDVPT 570

  Fly   545 TSAD------------------TKPSTLEELKHQPVIVEEGPVASKSRGNRAAYDLPGPSRKRPK 591
            :.:.                  .||::|...:.:|:|   ..|..|.|...|.:        .|.
Zfish   571 SGSGRKRLTRRKKQPNKSNLGLAKPASLMGKRLRPLI---SSVPKKKRTAAALF--------TPS 624

  Fly   592 LEVGLPVSKAADVEQAETKPAVVVEADDL--DEDA-DDPDVEPEAEPEATLQDMLNTGGDDDEFG 653
            :.:.||.: |.:...|.|.|. |:|:..:  ||.| ::.::|.||| |:.:..|...||::
Zfish   625 MTMMLPPA-APEPPPAPTPPP-VLESGPVAYDEPAEEEEEIEEEAE-ESCVSAMELIGGNE---- 682

  Fly   654 YGFDEEEE 661
            ||.:..||
Zfish   683 YGCEAVEE 690

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Brf	NP_001163636.1	TF_Zn_Ribbon	5..44	CDD:285471	23/37 (62%)
		SUA7	6..270	CDD:224323	159/264 (60%)
		CYCLIN	108..164	CDD:294043	34/55 (62%)
		CYCLIN	183..268	CDD:238003	61/84 (73%)
		BRF1	441..531	CDD:285040	50/92 (54%)
brf1b	NP_956183.1	SUA7	2..265	CDD:224323	157/261 (60%)
		TF_Zn_Ribbon	3..42	CDD:285471	23/37 (62%)
		CYCLIN	92..169	CDD:238003	46/76 (61%)
		CYCLIN	186..259	CDD:294043	50/72 (69%)
		BRF1	461..552	CDD:285040	50/92 (54%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170596668
Domainoid	1	1.000	110	1.000	Domainoid score	I6250
eggNOG	1	0.900	-	-		E1_COG1405
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1161
Inparanoid	1	1.050	480	1.000	Inparanoid score	I1427
OMA	1	1.010	-	-		QHG53031
OrthoDB	1	1.010	-	-		D364940at33208
OrthoFinder	1	1.000	-	-		FOG0002717
OrthoInspector	1	1.000	-	-		otm26253
orthoMCL	1	0.900	-	-		OOG6_101494
Panther	1	1.100	-	-	O	PTHR11618
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2091
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	15	14.770