DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ns1 and NUG1

DIOPT Version :9

Sequence 1:	NP_732199.2	Gene:	Ns1 / 42060	FlyBaseID:	FBgn0038473	Length:	581	Species:	Drosophila melanogaster
Sequence 2:	NP_010921.1	Gene:	NUG1 / 856723	SGDID:	S000000808	Length:	520	Species:	Saccharomyces cerevisiae

Alignment Length:	535	Identity:	183/535 - (34%)
Similarity:	267/535 - (49%)	Gaps:	91/535 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 RLKTKKSKRLTGRLKHKIEKKVRDHNKKERRAAKKNPKKGSKKQKLIQIPNICPFKDDILKEVEE 69
            |::.::|:|.:.:||..|:||...|.|||::.|||:....|:.:|...||:..|:|..||:|:|.
Yeast     2 RVRKRQSRRTSTKLKEGIKKKASAHRKKEKKMAKKDVTWRSRSKKDPGIPSNFPYKAKILEEIEA 66

  Fly    70 AKQRQEAER-LA---RREAFKAEREQNKFKTLESMVED-----------ADMRSTVHGIMHENDA 119
            .|.:...|| ||   |.||.||.:||......|.|:||           |...:..:.....|||
Yeast    67 KKMKDLEERELAKQQRLEARKAAKEQGVDAMDEDMIEDDENGLAALVESAQQAAAEYEGTPSNDA 131

  Fly   120 QDQDEKK---------YKNAVTKEQSL----KQYFKEFRKVIENADVVLEVVDARDPLGTRCNEV 171
            ..:|::.         |...|..|..|    |.|.|.|:.||:.:||:|.|:|||||..||..:|
Yeast   132 DVRDDELDVIDYNIDFYGEDVEGESELEKSRKAYDKIFKSVIDASDVILYVLDARDPESTRSRKV 196

  Fly   172 ERAVRGAPGNKRLVLVLNKADLVPRENLNNWIKYFRRSGPVTAFKASTQDQANRLGRRKLREMKT 236
            |.||..:.| |||:|:|||.||:|...|..|:.|.:.|.|....:||:.........|||.:..|
Yeast   197 EEAVLQSQG-KRLILILNKVDLIPPHVLEQWLNYLKSSFPTIPLRASSGAVNGTSFNRKLSQTTT 260

  Fly   237 EKAMQGSVCIGAELLMSMLGNYCRNKGIKTSIRVGVVGIPNVGKSSIINSL--TRG---RSCMVG 296
                       |..|:..|..|..|..:|.||.|||:|.|||||||:||:|  .||   ::|.||
Yeast   261 -----------ASALLESLKTYSNNSNLKRSIVVGVIGYPNVGKSSVINALLARRGGQSKACPVG 314

  Fly   297 STPGVTKSMQEVELDSKIKLIDCPGIVFTSGGENSHAV-------LKNAQRVGDVKDPFTIAESV 354
            :..|||.|::|:::|:|:|::|.|||.|.|..:....|       |.||.....:.||:.....:
Yeast   315 NEAGVTTSLREIKIDNKLKILDSPGICFPSENKKRSKVEHEAELALLNALPAKHIVDPYPAVLML 379

  Fly   355 LKRASK-----EYFCTMYDI-----TNYDTFEEFF----AKKAARMGKFLKKGVPDVVAAARSVL 405
            :||.:|     |.|..:|:|     .:.|||.:.|    |:|..|:|   |.|:|::.:|..|||
Yeast   380 VKRLAKSDEMTESFKKLYEIPPIPANDADTFTKHFLIHVARKRGRLG---KGGIPNLASAGLSVL 441

  Fly   406 NDWNTGKIKYCTQP-----PEVQEGQSVHI-------------SASIVHSEAREFDVEN-FESME 451
            |||..|||.....|     ...|:.|::..             .::||...::|||::. |.|::
Yeast   442 NDWRDGKILGWVLPNTSAAASQQDKQNLSTINTGTKQAPIAANESTIVSEWSKEFDLDGLFSSLD 506

  Fly   452 TEILEHCAVKTDDIM 466
            ..|   .|.|..|.|
Yeast   507 KAI---DASKDQDTM 518

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ns1	NP_732199.2	GN3L_Grn1	17..88	CDD:285863	31/74 (42%)
		SurA_N_3	59..>150	CDD:304439	36/118 (31%)
		RbgA	121..443	CDD:224083	129/378 (34%)
		Nucleostemin_like	152..322	CDD:206753	74/174 (43%)
		Ras_like_GTPase	271..>400	CDD:206648	56/154 (36%)
NUG1	NP_010921.1	GN3L_Grn1	15..88	CDD:400856	30/72 (42%)
NUG1	NP_010921.1	RbgA	162..481	CDD:224083	121/333 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157345420
Domainoid	1	1.000	87	1.000	Domainoid score	I1857
eggNOG	1	0.900	-	-		E1_COG1161
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	228	1.000	Inparanoid score	I737
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002318
OrthoInspector	1	1.000	-	-		oto99070
orthoMCL	1	0.900	-	-		OOG6_101399
Panther	1	1.100	-	-	LDO	PTHR11089
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R524
SonicParanoid	1	1.000	-	-		X1530
TreeFam	1	0.960	-	-
	13	12.780