DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Ns1 and Gnl3

DIOPT Version :9

Sequence 1:	NP_732199.2	Gene:	Ns1 / 42060	FlyBaseID:	FBgn0038473	Length:	581	Species:	Drosophila melanogaster
Sequence 2:	NP_705775.2	Gene:	Gnl3 / 30877	MGIID:	1353651	Length:	538	Species:	Mus musculus

Alignment Length:	594	Identity:	202/594 - (34%)
Similarity:	319/594 - (53%)	Gaps:	73/594 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 LKRLKTKK-SKRLTGRLKHKIEKKVRDHNKKERRAAKKNPKKGSKK-QKLIQIPNICPFKDDILK 65
            :||.|.|| |||:|...::||:||||:|::|.|:.||   |:|.|| :|...:||..|||:.:|:
Mouse     1 MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAK---KRGHKKPRKDPGVPNSAPFKEALLR 62

  Fly    66 EVEEAKQRQEAERLARREAFKAEREQNKFKTLESMVEDADMRSTVHGIMHENDAQDQDEKKYKNA 130
            |.|  .::|:.|.|.:::....::||.:.:.||  |...|.:|.|      ...::.||.|.|.|
Mouse    63 EAE--LRKQQLEELKQQQKLDRQKEQERKRKLE--VSPGDEQSNV------ETREESDEPKRKKA 117

  Fly   131 VTKEQSLKQ-YFKEFRKVIENADVVLEVVDARDPLGTRCNEVERAVRGAPGNKRLVLVLNKADLV 194
            ...:|:.|: :.:|.:||||.:|:||||:|||||||.||.::|.||..: |:|:|:|||||:|||
Mouse   118 KAGKQNPKKLHCQELKKVIEASDIVLEVLDARDPLGCRCPQIEEAVIQS-GSKKLILVLNKSDLV 181

  Fly   195 PRENLNNWIKYFRRSGPVTAFKASTQDQANRLGRRKLREMKTEKAM--QGSVCIGAELLMSMLGN 257
            |:|||.||:.|..:..|...|||||     .|..||..::|.:|.:  |..:|.|.|.|..:||:
Mouse   182 PKENLENWLNYLNKELPTVVFKAST-----NLKNRKTFKIKKKKVVPFQSKICCGKEALWKLLGD 241

  Fly   258 YCRNKGIKTSIRVGVVGIPNVGKSSIINSLTRGRSCMVGSTPGVTKSMQEVELDSKIKLIDCPGI 322
            :.::.|  ..|:|||:|.|||||||:||||.:...|.||.:.|:|:|||.|.||.:|.:||.|.:
Mouse   242 FQQSCG--KDIQVGVIGFPNVGKSSVINSLKQEWICNVGISMGLTRSMQIVPLDKQITIIDSPCL 304

  Fly   323 VFTSGGENSHAVLKNAQRVGDVKDPFTIAESVLKRASKEYFCTMYDITNYDTFEEFFAKKAARMG 387
            :.:.....:...|::...:.::: |...|.::|.:|..|.....|.:..|.....||.|.|.|.|
Mouse   305 IISPCNSPTALALRSPASIEELR-PLEAASAILSQADNEQVVLKYTVPEYKDSLHFFTKLAQRRG 368

  Fly   388 KFLKKGVPDVVAAARSVLNDWNTGKIKYCTQPPEVQEGQSVHISASIVHSEAREFDVENFESMET 452
            ...|.|.|:|.:||:.|.::|....:.|...|| .....|:|.:.:|.....:.|::|..|....
Mouse   369 LHQKGGSPNVESAAKLVWSEWTGASLGYYCHPP-ASWNHSLHFNENIAAVMKKGFNLEELEKNNA 432

  Fly   453 EILE-----HCAVKTDDIMEITSTGPLEIRQPREEAEPADKITASLVIDEK----EKPAKGRKRK 508
            ..::     |.   |:.|: ..|:|                 ..:.::|||    |.|      :
Mouse   433 HSIQVLKGPHL---TNRIL-FRSSG-----------------LTNGILDEKDIVEESP------R 470

  Fly   509 LDEEKEKVDPSLLLEENQS--LNKGIKQMQKLKKKQNVRNEKKIS-KITDVLDSFSLGPSSSKAE 570
            ..|:|:..|.    :||.|  .|..|..:..:::.:.:..|:..: |.:|  .|.:|..:|.:.|
Mouse   471 QTEDKQDADD----QENGSGERNAEISDVAPVEETRELSPEQSTAGKPSD--GSSALDRASQEDE 529

  Fly   571 KYDFDEDYV 579
            .|||..||:
Mouse   530 TYDFTTDYI 538

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ns1	NP_732199.2	GN3L_Grn1	17..88	CDD:285863	27/71 (38%)
		SurA_N_3	59..>150	CDD:304439	27/91 (30%)
		RbgA	121..443	CDD:224083	125/324 (39%)
		Nucleostemin_like	152..322	CDD:206753	85/171 (50%)
		Ras_like_GTPase	271..>400	CDD:206648	47/128 (37%)
Gnl3	NP_705775.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..57		28/58 (48%)
		Basic. /evidence=ECO:0000250	2..46		24/46 (52%)
		GN3L_Grn1	16..93	CDD:285863	29/81 (36%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	69..126		17/64 (27%)
		GTPase_YlqF	130..395	CDD:274669	112/273 (41%)
		Nucleostemin_like	140..302	CDD:206753	84/169 (50%)
		Intermediate. /evidence=ECO:0000250	277..451		48/179 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	460..538		24/89 (27%)
		Acidic. /evidence=ECO:0000250	460..532		20/83 (24%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG1161
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1210675at2759
OrthoFinder	1	1.000	-	-		FOG0002318
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101399
Panther	1	1.100	-	-	O	PTHR11089
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R524
SonicParanoid	1	1.000	-	-		X1530
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	10	9.810