Protein Alignment Dad and SMAD4

DIOPT Version :10

• Sequence 1: NP_001369021.1
• Gene: Dad / 42059
• FlyBaseID: FBgn0020493
• Length: 665
• Species: Drosophila melanogaster
• Sequence 2: NP_005350.1
• Gene: SMAD4 / 4089
• HGNCID: 6770
• Length: 552
• Species: Homo sapiens
• Alignment Length: 569
• Identity: 125/569 - (21%)
• Similarity: 188/569 - (33%)
• Gaps: 222/569 - (39%)

  Fly   185 TESTSGSTLTIPVSTSRATAHPPQTQAQNGKRFREDF-----EALMKQLKRKQRNELLLAVKSRL 244
            |.:::.:.|:|        .|......|.|:  .|.|     |:|:|:||.|         |..|
Human     9 TPTSNDACLSI--------VHSLMCHRQGGE--SETFAKRAIESLVKKLKEK---------KDEL 54

  Fly   245 DPPTKTQRDVVEPTTTTAPTYLQCILI----PCKTQTVWE---PHVTASRLFFWRELWNAKELKR 302
            |       .::...||......:|:.|    ..:.|....   |||..:||:.|.:| :..|||.
Human    55 D-------SLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDL-HKNELKH 111

  Fly   303 LPTCPAARD--CIYMCCNPLHWFRIL--------------------------------------- 326
            :..|..|.|  |..:|.||.|:.|::
Human   112 VKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEG 176

  Fly   327 -------HQPETESPTPPYQRSKMLRLKDADFEEDSQNDAKSAA------LSTWSAESTSISNIY 378
                   |.|...:.|..|....:|        ..|:::|.|.|      :::.|..::.:...:
Human   177 HSIQTIQHPPSNRASTETYSTPALL--------APSESNATSTANFPNIPVASTSQPASILGGSH 233

  Fly   379 ----------------------KPALY--ESVT----------TDGKDHNINS------------ 397
                                  :||.|  .|.|          |....|:.|.
Human   234 SEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHP 298

  Fly   398 ----------------------QVWCQIAYWEMAHRVGEFFHAKTNAVNIYTDGIV-ASEVDSMC 439
                                  :.||.|||:||..:|||.|...::...:..||.| .|..|..|
Human   299 GHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFC 363

  Fly   440 LRDLTPAGNQIH--SVVPKARHTVGLGVTLSLE-NGDVWIYNRGNTTIFVDSPTLSENL-----D 496
            |..|    :.:|  ..:.:||..:|.||.|..: .||||:....:..:||.|..|....     |
Human   364 LGQL----SNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGD 424

  Fly   497 RVCKVMPGYCLKAFETNR---------------AELLSMRDHGHHPMGP---------------- 530
            .|.|:.|...:|.|:..:               |...:....|:.| ||
Human   425 AVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIP-GPGSVGGIAPAISLSAAA 488

  Fly   531 ---VDYFS----IKISFGKGWGRDYKRQDIMGCPCWLEVHFSHLRXQYL 572
               ||...    :::||.||||.||.||.|...|||:|:|. |...|.|
Human   489 GIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHL-HRALQLL 536

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Dad	NP_001369021.1	MH1_SMAD_6_7	197..329	CDD:199813	39/191 (20%)
		MH2_I-SMAD	400..565	CDD:199821	63/211 (30%)
SMAD4	NP_005350.1	Mediates interaction with ZBTB7A. /evidence=ECO:0000269|PubMed:25514493	1..322		59/347 (17%)
		MH1_SMAD_4	14..138	CDD:199816	40/150 (27%)
		Required for interaction with TSC22D1. /evidence=ECO:0000269|PubMed:15881652	44..69		9/40 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	168..194		3/25 (12%)
		Pneumo_att_G	<176..>281	CDD:114270	16/112 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	236..256		0/19 (0%)
		SAD	275..320		3/44 (7%)
		MH2_SMAD_4	320..541	CDD:199823	66/223 (30%)