Protein Alignment Dad and Smad4

DIOPT Version :9

• Sequence 1: NP_001369021.1
• Gene: Dad / 42059
• FlyBaseID: FBgn0020493
• Length: 665
• Species: Drosophila melanogaster
• Sequence 2: NP_032566.2
• Gene: Smad4 / 17128
• MGIID: 894293
• Length: 551
• Species: Mus musculus
• Alignment Length: 572
• Identity: 124/572 - (21%)
• Similarity: 184/572 - (32%)
• Gaps: 229/572 - (40%)

  Fly   185 TESTSGSTLTIPVSTSRATAHPPQTQAQNGKRFREDF-----EALMKQLKRKQRNELLLAVKSRL 244
            |.:::.:.|:|        .|......|.|:  .|.|     |:|:|:||.|         |..|
Mouse     9 TPTSNDACLSI--------VHSLMCHRQGGE--SETFAKRAIESLVKKLKEK---------KDEL 54

  Fly   245 DPPTKTQRDVVEPTTTTAPTYLQCILI----PCKTQTVWE---PHVTASRLFFWRELWNAKELKR 302
            |       .::...||......:|:.|    ..:.|....   |||..:||:.|.:| :..|||.
Mouse    55 D-------SLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDL-HKNELKH 111

  Fly   303 LPTCPAARD--CIYMCCNPLHWFRIL--------------------------------------- 326
            :..|..|.|  |..:|.||.|:.|::
Mouse   112 VKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSMLVKDEYVHDFEGQPSLPTEGH 176

  Fly   327 ------HQPETESPTPPYQRSKMLRLKDADFEEDSQNDAKSAALSTWSAESTSISNIYKPAL--- 382
                  |.|...:.|..|....:|.            .|:|.|.||.:..:..:::..:||.
Mouse   177 SIQTIQHPPSNRASTETYSAPALLA------------PAESNATSTTNFPNIPVASTSQPASILA 229

  Fly   383 -------------------------------YESVT----------TDGKDHNINS--------- 397
                                           :.|.|          |....|:.|.
Mouse   230 GSHSEGLLQIASGPQPGQQQNGFTAQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMP 294

  Fly   398 -------------------------QVWCQIAYWEMAHRVGEFFHAKTNAVNIYTDGIV-ASEVD 436
                                     :.||.|||:||..:|||.|...::...:..||.| .|..|
Mouse   295 PHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPSGGD 359

  Fly   437 SMCLRDLTPAGNQIH--SVVPKARHTVGLGVTLSLE-NGDVWIYNRGNTTIFVDSPTLSENL--- 495
            ..||..|    :.:|  ..:.:||..:|.||.|..: .||||:....:..:||.|..|....
Mouse   360 RFCLGQL----SNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRA 420

  Fly   496 --DRVCKVMPGYCLKAFETNR---------------AELLSMRDHGHHPMGP------------- 530
              |.|.|:.|...:|.|:..:               |...:....|:.| ||
Mouse   421 PGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIP-GPGSVGGIAPAISLS 484

  Fly   531 ------VDYFS----IKISFGKGWGRDYKRQDIMGCPCWLEVHFSHLRXQYL 572
                  ||...    :::||.||||.||.||.|...|||:|:|. |...|.|
Mouse   485 AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHL-HRALQLL 535

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Dad	NP_001369021.1	MH1_SMAD_6_7	197..329	CDD:199813	39/190 (21%)
		MH2_I-SMAD	400..565	CDD:199821	63/211 (30%)
Smad4	NP_032566.2	Mediates interaction with ZBTB7A. /evidence=ECO:0000250|UniProtKB:Q13485	1..321		58/350 (17%)
		MH1_SMAD_4	14..138	CDD:199816	40/150 (27%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	166..192		2/25 (8%)
		SAD	274..319		3/44 (7%)
		MH2_SMAD_4	319..540	CDD:199823	66/223 (30%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167834114
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3701
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.740