DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pak3 and SKM1

DIOPT Version :9

Sequence 1:	NP_001262638.1	Gene:	Pak3 / 41995	FlyBaseID:	FBgn0044826	Length:	583	Species:	Drosophila melanogaster
Sequence 2:	NP_014528.1	Gene:	SKM1 / 854036	SGDID:	S000005473	Length:	655	Species:	Saccharomyces cerevisiae

Alignment Length:	611	Identity:	188/611 - (30%)
Similarity:	300/611 - (49%)	Gaps:	145/611 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 ISEIGAPTNFQRHFHVSRNQETGDLEGLPAPWVRLM-NSQITRDEQDKNPDAAYHAVKYY----- 75
            :|.:.:||||....||..:.:.|:..|:|..|.:|: .|:||.|:.::|..|...|:::|
Yeast   120 LSGVSSPTNFTHKVHVGFDPKVGNFVGVPDSWAKLLQTSEITYDDWNRNSKAVIKALQFYEDYNG 184

  Fly    76 -----------------------NYSIKKKENEV-------FKPFITEDVIHEESKEIENYVNYK 110
                                   .|.|.|:.|.:       .:...|:.::.....|::.:
Yeast   185 LDTMQFNDHLNTSLDLKPLKSPTRYIINKRTNSIKRSVSRTLRKGKTDSILPVYQSELKPF---- 245

  Fly   111 NKHKSQDPEKSDDDGSSTATETESSSGCGSSAGNSNSSSINDSSQQSTVPLDALEEVFKELKTNL 175
                   |..||||...|..|.......|....:..|::.:.:.|     |...|:  |.::::|
Yeast   246 -------PRPSDDDYKFTNIEDNKVREEGRVHVSKESTADSQTKQ-----LGKKEQ--KVIQSHL 296

  Fly   176 ---EHRETLKAAPQEPPPVPPKKSPHTIPPKPQIKPKPRVTQKFSRTSDIRRDEDSDNQ-HKINT 236
               ::..|.:              ||.:.|                ::...::.||..: ||  .
Yeast   297 RRHDNNSTFR--------------PHRLAP----------------SAPATKNHDSKTKWHK--E 329

  Fly   237 DTIIIKPAVGAQDAGADDNPDETILRRSKEKRAQKTDAEIYVELRAICNSDDPRERYKTTQEVGK 301
            |.:.:|           :|.|..               ||.::::.:....:||..::..::.|:
Yeast   330 DLLELK-----------NNDDSN---------------EIIMKMKTVAIDVNPRPYFQLVEKAGQ 368

  Fly   302 GASGIVFIAADL----QNE-------------SQVAVKTIDMKNQSSKDLILTEIRVLKDFNHKN 349
            ||||.|:::..:    :|:             .:||:|.|.:..|..|.||:.|:.|:.|...:|
Yeast   369 GASGAVYLSKRIKLPQENDPRFLKSHCHRVVGERVAIKQIRLSEQPKKQLIMNELLVMNDSRQEN 433

  Fly   350 LVNFLDAYLLEPEDQLWVVMEYMDGGPLTDVVT----------ETVMKERQIACVCRETLYAISF 404
            :||||:||::: :::|||:||||:||.|||::.          .:.:.|.|:|.:.:||...:.|
Yeast   434 IVNFLEAYIID-DEELWVIMEYMEGGCLTDILDAVARSNTGEHSSPLNENQMAYIVKETCQGLKF 497

  Fly   405 LHAKGIIHRDIKSDNVLLGMDGSVKVTDFGFCANI-EGDEKRQTMVGTPYWMAPEVVTRKKYGKK 468
            ||.|.||||||||||:||...|.||:||||||..: |...||.||||||||||||:|.:|.|.:|
Yeast   498 LHNKKIIHRDIKSDNILLNSQGLVKITDFGFCVELTEKRSKRATMVGTPYWMAPEIVNQKGYDEK 562

  Fly   469 VDIWSIGIMAIEMIEGQPPYLYETPLRALYLIAANGRPDIKSWDKLSPNLQDFLDRCLQVEVDRR 533
            ||:||:|||.||||||:||||.|.||:||||||.||.|.::..:.:|...:.|||.||||.|:.|
Yeast   563 VDVWSLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSPKLRHPESVSKQTKQFLDACLQVNVESR 627

  Fly   534 ATADELLSHPFLNDCSEVKALVPNIK 559
            |:..:||:..||:.....:.|..::|
Yeast   628 ASVRKLLTFEFLSMACSPEQLKVSLK 653

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pak3	NP_001262638.1	PBD	19..73	CDD:279166	18/54 (33%)
		PKc_like	288..545	CDD:304357	130/284 (46%)
		S_TKc	293..545	CDD:214567	128/279 (46%)
SKM1	NP_014528.1	PH_Cla4_Ste20	4..113	CDD:270097
		PBD	122..180	CDD:395634	18/57 (32%)
		STKc_PAK	359..640	CDD:270789	129/281 (46%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0578
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000763
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100484
Panther	1	1.100	-	-	O	PTHR48015
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	7	6.770