Protein Alignment ss and Clock

DIOPT Version :10

• Sequence 1: NP_001163629.2
• Gene: ss / 41988
• FlyBaseID: FBgn0003513
• Length: 1015
• Species: Drosophila melanogaster
• Sequence 2: NP_001376183.1
• Gene: Clock / 60447
• RGDID: 620271
• Length: 864
• Species: Rattus norvegicus
• Alignment Length: 1034
• Identity: 229/1034 - (22%)
• Similarity: 363/1034 - (35%)
• Gaps: 291/1034 - (28%)

  Fly    28 VTKSNPSKRHRERLNAELDLLASLLPFEQNILSKLDRLSILRLSVSYLRTKSYFQVVMHKDKEDN 92
            |:::...|:.|::.|..:..|.|:||....   |:|:.::|:.|:.:||          |.||
  Rat    37 VSRNKSEKKRRDQFNVLIKELGSMLPGNAR---KMDKSTVLQKSIDFLR----------KHKE-- 86

  Fly    93 GVLPHIHAH-DGYRTRELGAFEHGLLDGDMF----LQALNGFLMILTCEGEVFFATHSIESYLGF 152
                 |.|. |....|:  .::...|..:.|    |:||:||.:.:..:|.|.:.:.::.|.|..
  Rat    87 -----ITAQSDASEIRQ--DWKPTFLSNEEFTQLMLEALDGFFLAIMTDGSVVYVSETVTSLLEH 144

  Fly   153 HQSDIVHQSVYELV----HSEDREELQRQLL-WNSFLPADMSSMQLAE--------TLAP-DKAL 203
            ..||:|.||::..:    |||..:.|...|| .:|..|.|:.|....|        |:.| :.:.
  Rat   145 LPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEDLKSKNQLEFCCHMLRGTIDPKEPST 209

  Fly   204 YLERSFTVRFRCLLDNTSGFLRLDIRGRIKVLHGQNRKTEEPPLALFAYCTPFGPPSLLEIPHKE 268
            |....|...|:. |::.|........|.|:..|..:.:.....:|.....||.....:..:....
  Rat   210 YEYVRFIGNFKS-LNSVSTSTHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPN 273

  Fly   269 NMFKSKHKLDFSLVSMDQRGKHILGYADAELVNMGGYDLVHYDDLAYVASAHQELLKTGASGMIA 333
            ..|.|:|.|::..:.:|.|...|:||...|::...|||..|.|||..:|..|:.|::.|......
  Rat   274 EEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLESLAKCHEHLMQYGKGKSCY 338

  Fly   334 YRYQKKDGEWQWLQTSSRLVYK--NSKPDFVICTH-------------RQL-MDEEGHDLLGKRT 382
            ||:..|..:|.||||...:.|.  ||:|:|::|||             |:| ::|...:....::
  Rat   339 YRFLTKGQQWIWLQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRRELGIEESLPETAADKS 403

  Fly   383 MDFKVSYLDTGLASTYFSEA----DQLVVPPSTS----PTAHAL--PPPVTPTRPNRRYKTQLRD 437
            .|   |..|..:.:....||    |....|.::|    .::|..  .|..|||:......|..|.
  Rat   404 QD---SGSDNRINTVSLKEALERFDHSPTPSASSRSSRKSSHTAVSDPSSTPTKIPTDTSTPPRP 465

  Fly   438 FLSTCRSKRKLQQQNQPQTQQTSPLGGQ------VGSPAPAVAVEYLPDPAAAVAAAYSNLNPMY 496
            .|..          ::..||:.|....|      |||.
  Rat   466 HLPA----------HEKMTQRRSSFSSQSINSQSVGSS--------------------------- 493

  Fly   497 TTSPYASAADNLYMGSSMPANAFYPVSENLFHQYRLQGAVGGYYTDYPHSGAPASAYVANGFLSY 561
            .|.|..|.|.||            |:.:.: .|::|...:|            |..::.:. |..
  Rat   494 LTQPAMSQAANL------------PIPQGM-SQFQLSAQLG------------AMQHLKDQ-LEQ 532

  Fly   562 DGYAIASKADEKWQETGKYYSGYSSGYGSPTSTPQQIPLKTPKSSPQVMEVISCSSDGPSPVGGA 626
            ....|.:....:.:|..|........:|      |.:.:...:|:|.:.......|.|.|.:...
  Rat   533 RTRMIEANIHRQQEELRKIQEQLQMVHG------QGLQMFLQQSNPGLNFGSVQLSSGNSNIQQL 591

  Fly   627 TP-NGVGSVTPKVEL-AGTTAA-AAAGQDPYERQTVLMWGTTHSSGVPLNSHGGRTGAGNPASPQ 688
            || |..|.|.|..:: :|..|. .:.||...::||:....|..|....::.|...|...|.....
  Rat   592 TPINMQGQVVPVNQIQSGVNAGHVSTGQHMIQQQTLQSTSTQQSQQSVMSGHSQPTSLPNQTPST 656

  Fly   689 RSTPLGNGLGYAS----------ANNNNNDLEPATAAKWNGTKEL---------------PGKSG 728
            .:.||.|.:..:.          ::...|..:.||...:...:::               |...|
  Rat   657 LTAPLYNTMVISQPAAGSMVPIPSSMPQNSTQSATVTTFTQDRQIRFSQGQQLVTKLVTAPVACG 721

  Fly   729 SASTPESYQMQHDDSGLYSASSHTTSPQQQQQQQLQQSQRGVGSNVSAPSSSLTSTGTDQQAVHP 793
            :...|.:..|     |....:..|.:.||||.|.|                |:|.....||
  Rat   722 AVMVPSTMLM-----GQVVTAYPTFATQQQQAQAL----------------SVTQQQQQQQ---- 761

  Fly   794 SSCHQQQQQQQQQHHHAHPHPHSHHHHHHHHHHETAHQHSSEIILTTHHQQQSQQQQQSLAGYPL 858
               .|||||||||...|.|.|.|                          ||.||.|..
  Rat   762 ---QQQQQQQQQQPQQAQPQPQS--------------------------QQSSQDQPH------- 790

  Fly   859 HHQLVGVGSGSPPPQPPVALSRSPTTLPAVSSLLNGSASAGGASDIPYQQLAIVSA-PLVICAEE 922
                       |..|.|..|::.|......|.||:|:         |..||.:.:| ||
  Rat   791 -----------PSVQQPAQLTQPPQQFLQTSRLLHGN---------PSTQLILSAAFPL------ 829

  Fly   923 VGGGVSSIGRKSSKQQQSLQHLQQQQSLQQQQHAIYQQQHHHSQHHPHH--QLLYPANI 979
                                   ||.:.....|    |||...|.|.|.  .|..|:.:
  Rat   830 -----------------------QQSTFPPSHH----QQHQQQQLHRHRTDSLTDPSKV 861

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ss	NP_001163629.2	bHLH-PAS_spineless_like	24..87	CDD:381573	14/58 (24%)
		PAS	123..178	CDD:214512	18/58 (31%)
		PAS_3	282..366	CDD:430001	31/85 (36%)
Clock	NP_001376183.1	bHLH-PAS_CLOCK	29..89	CDD:381577	18/71 (25%)
		Nuclear localization signal. /evidence=ECO:0000250|UniProtKB:O08785	32..47		2/9 (22%)
		PAS	109..202	CDD:395786	26/92 (28%)
		PAS_11	274..377	CDD:464214	37/102 (36%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	388..497		26/148 (18%)
		Interaction with SIRT1. /evidence=ECO:0000250|UniProtKB:O08785	450..570		30/188 (16%)
		Implicated in the circadian rhythmicity. /evidence=ECO:0000250	514..564		9/68 (13%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	824..864		14/71 (20%)