Protein Alignment ss and Hif3a

DIOPT Version :10

• Sequence 1: NP_001163629.2
• Gene: ss / 41988
• FlyBaseID: FBgn0003513
• Length: 1015
• Species: Drosophila melanogaster
• Sequence 2: NP_001156422.1
• Gene: Hif3a / 53417
• MGIID: 1859778
• Length: 664
• Species: Mus musculus
• Alignment Length: 683
• Identity: 162/683 - (23%)
• Similarity: 253/683 - (37%)
• Gaps: 210/683 - (30%)

  Fly    30 KSNPSKRHRERLNAE-LDLLASLLPFEQNILSKLDRLSILRLSVSYLRTKSYFQVVMHK------ 87
            ||..:.|.|.....| |..||..|||.:.:.:.||:.||:||::||||        ||:
Mouse    18 KSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLTISYLR--------MHRLCAAGE 74

  Fly    88 -DKEDNGVLPHIHAHDGYRTRELGAFEHGLLDGDMFLQALNGFLMILTCEGEVFFATHSIESYLG 151
             ::.:.|..|                    ||. .:|:||.||:|:||.||::.:.:.::..:||
Mouse    75 WNQVEKGGEP--------------------LDA-CYLKALEGFVMVLTAEGDMAYLSENVSKHLG 118

  Fly   152 FHQSDIVHQSVYELVHSEDREELQRQLLWNSFLPADMSSMQLAETLAPDKALYLERSFTVRFRCL 216
            ..|.:::..|:::.:|..|:||||     ::..|....|.:..|  ||     .||.|::|.:..
Mouse   119 LSQLELIGHSIFDFIHPCDQEELQ-----DALTPRPNLSKKKLE--AP-----TERHFSLRMKST 171

  Fly   217 LDNTSGFLRLDIRGRI-KVLH--GQNR-------------KTEEPPL-ALFAYCTPFGPPSLLEI 264
            |  ||....|:::... ||||  |..|             ...|||| .|...|.....|:.||.
Mouse   172 L--TSRGRTLNLKAATWKVLHCSGHMRAYKPPAQTSPAGSPRSEPPLQCLVLICEAIPHPASLEP 234

  Fly   265 PHKENMFKSKHKLDFSLVSMDQRGKHILGYADAELVNMGGYDLVHYDDLAYVASAHQELLKTGAS 329
            |.....|.|:|.||......|:|...:.||:..:|:....|:.:|..|...|:.:...||..|.:
Mouse   235 PLGRGAFLSRHSLDMKFTYCDERIAEVAGYSPDDLIGCSAYEYIHALDSDAVSRSIHTLLSKGQA 299

  Fly   330 GMIAYRYQKKDGEWQWLQTSSRLVYKNSKP--DFVICTHRQL--MDEEGHDLLGKRT-------- 382
            ....||:..:.|.:.|.||.:.:|.....|  :.:||.|..:  ::|.|..|..::|
Mouse   300 VTGQYRFLARTGGYLWTQTQATVVSGGRGPQSESIICVHFLISRVEETGVVLSLEQTEQHTRRPP 364

  Fly   383 --------------MDFKVSYLDTGLASTYFSEADQLVVPPS--TSPTAHAL--------PPPVT 423
                          :|.....:...|.....||| .|...|.  .||....|        ||..|
Mouse   365 RLSASSQKGIPGNSVDSPAPRILAFLHPPALSEA-SLAADPRRFCSPDLRRLMAPILDGPPPAAT 428

  Fly   424 PTRPNRRYKTQLRDFLSTCRSKRKLQQQNQPQTQQTSPLGGQVGSPAPAVAVEYLPDP------- 481
            |:.|                     |...:||            ||.||    .|||.
Mouse   429 PSTP---------------------QATRRPQ------------SPLPA----DLPDKLTVGLEN 456

  Fly   482 AAAVAAAYSNLN--------------PMYTTSPYASAADNLYMGSS--MP--------------A 516
            |..::.|..|..              .:...:||.|..|:..:.||  :|              |
Mouse   457 AHRLSTAQKNKTVETDLDIAQDPDTLDLEMLAPYISMDDDFQLNSSEQLPKVHRRPPRVARRPRA 521

  Fly   517 NAFY----PVSE-NLFHQYRLQGAVGGYYTDYPHSGAPASAYVANGFLSYDGYAIASKADEKWQE 576
            .:|:    |:.| :|..::   |:........|..|...:|.:..|...   .|:|..:::|..|
Mouse   522 RSFHGLSPPIPEPSLLPRW---GSDPRLNCSSPSRGDRPTASLMPGTRK---RALAQSSEDKGLE 580

  Fly   577 TGKYYSGYSSGYGSPTSTPQQIPLKTPKSSPQV 609
            .                    :..|.||.||::
Mouse   581 L--------------------LETKPPKRSPRL 593

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ss	NP_001163629.2	bHLH-PAS_spineless_like	24..87	CDD:381573	22/57 (39%)
		PAS	123..178	CDD:214512	20/54 (37%)
		PAS_3	282..366	CDD:430001	22/85 (26%)
Hif3a	NP_001156422.1	bHLH-PAS_HIF3a_PASD7	12..74	CDD:381572	23/63 (37%)
		PAS	90..145	CDD:214512	20/59 (34%)
		PAS_3	251..338	CDD:430001	22/86 (26%)
		HIF-1	475..506	CDD:463274	6/30 (20%)