Protein Alignment ss and hif1ab

DIOPT Version :10

• Sequence 1: NP_001163629.2
• Gene: ss / 41988
• FlyBaseID: FBgn0003513
• Length: 1015
• Species: Drosophila melanogaster
• Sequence 2: NP_001296971.1
• Gene: hif1ab / 393202
• ZFINID: ZDB-GENE-040426-706
• Length: 777
• Species: Danio rerio
• Alignment Length: 800
• Identity: 181/800 - (22%)
• Similarity: 295/800 - (36%)
• Gaps: 236/800 - (29%)

  Fly     4 LGTVYATKRRRRNGKSLKPPPKDGVTKSNPSKRHRERLNAELDL---LASLLPFEQNILSKLDRL 65
            :.|...|:::|.:.:..|...:|..         |.|...|.::   ||..||...|:.|.||:.
Zfish     1 MDTGVVTEKKRVSSERRKEKSRDAA---------RSRRGKESEVFYELAHQLPLPHNVTSHLDKA 56

  Fly    66 SILRLSVSYLRTKSYFQVVMHKDKEDNGVLPHIHAHDGYRTRELGAFEHGLLDGDMFLQALNGFL 130
            ||:||::||||.:........::||:|               ||.:..:|     .:|:||.|||
Zfish    57 SIMRLTISYLRMRKLLNSDEKEEKEEN---------------ELESQLNG-----FYLKALEGFL 101

  Fly   131 MILTCEGEVFFATHSIESYLGFHQSDIVHQSVYELVHSEDREELQRQLLWNSFLPADMSSMQLAE 195
            |:|:.:|::.:.:.::...:|..|.|:...|::|..|..|.|||:..|:..:      .|.:..|
Zfish   102 MVLSEDGDMVYLSENVSKSMGLTQFDLTGHSIFEFSHPCDHEELREMLVHRT------GSKKTKE 160

  Fly   196 TLAPDKALYLERSFTVRFRCLLDNTSGFLRLDIRGRI--------KVLH--GQNRKTE------- 243
            .       ..||||.:|.:|.|.:         |||.        ||||  |..|..|
Zfish   161 Q-------NTERSFFLRMKCTLTS---------RGRTVNIKSATWKVLHCAGHVRVHEGSEASED 209

  Fly   244 ----EPPLA-LFAYCTPFGPPSLLEIPHKENMFKSKHKLDFSLVSMDQRGKHILGYADAELVNMG 303
                |||:. |...|.|...||.:|:|.....|.|:|.||......|:|...::||...:|:|..
Zfish   210 SGFKEPPVTYLVLICEPIPHPSNIEVPLDSKTFLSRHTLDMKFSYCDERITELMGYEPDDLLNRS 274

  Fly   304 GYDLVHYDDLAYVASAHQELLKTGASGMIAYRYQKKDGEWQWLQTSSRLVY--KNSKPDFVIC-- 364
            .|:..|..|..::...|..|...|.:....||...|.|.:.|::|.:.::|  |||:|..::|
Zfish   275 VYEYYHALDSDHLTKTHHNLFAKGQATTGQYRMLAKKGGFVWVETQATVIYNPKNSQPQCIVCVN 339

  Fly   365 -------------------THRQLMDEEGHDLLGKRT-MD----FKVSYLDTGLASTY------- 398
                               |..:.:::|..:...|.: :|    ||...|:..|.|:.
Zfish   340 YVLSGIVEGDVVLSLQQTVTEPKAVEKESEETEEKTSELDILKLFKPESLNCSLESSTLYNKLKE 404

  Fly   399 --------------------FSEAD---QLV--VP-------PSTSPTAHALPPPVTPTRPN--- 428
                                |:.:|   ||:  ||       ||:|   ..||..::|..|:
Zfish   405 EPEALTVLAPAAGDAIISLDFNNSDSDIQLLKEVPLYNDVMLPSSS---EKLPLSLSPLTPSDSI 466

  Fly   429 ---RRYKTQLRDFLSTCRSKRKLQQQNQPQTQ------QTSPLGGQVGSPA-PAVAVEYLPDPAA 483
               .:.:|...||..:..|.|.....|.|.|.      ..||:  ..|.|. |.::.|:..|...
Zfish   467 PALTKLETGGEDFPFSSASDRVPDPTNTPSTSGLGSSGPNSPM--DYGFPVEPDISSEFKLDLVE 529

  Fly   484 AVAAAYSNLNPMYTTSPYASAADNLYMGSSMPANAFYPVSENLFHQYRLQGAVGGYYTDYPHSGA 548
            .:.|..:.....::|.|    .::|.:....|   :.|:.::.  |.|:..         |....
Zfish   530 KLFAIDTEAKTPFSTQP----MEDLDLEMLAP---YIPMDDDF--QLRIPS---------PLDPL 576

  Fly   549 PASAYVANGFLSYDGYAIASKADEKWQETGKYYSGYSSGYGSPTST-----PQQIPLKTPKSSPQ 608
            |::.:..:...|                   .:....|...||.|:     .|:...:.|.....
Zfish   577 PSATHSVSAMSS-------------------LFQPLPSSPASPASSTSSTVKQEASSRAPSPLHL 622

  Fly   609 VMEVISC------SSDGPSPVGGATP---------------------------NGVGSVTPKVEL 640
            :.||.|.      .|...|||..:||                           |.|.|::..|.|
Zfish   623 LQEVCSAPVSPFSGSRDASPVRSSTPQSSSQLNNREMSPKMLAFQNIQRKRKLNEVTSLSEAVGL 687

  Fly   641 AGTTAAAAAGQDPYERQTVL 660
            .....:..:..||.:|..||
Zfish   688 GALLHSVDSAIDPGKRAKVL 707

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ss	NP_001163629.2	bHLH-PAS_spineless_like	24..87	CDD:381573	20/65 (31%)
		PAS	123..178	CDD:214512	19/54 (35%)
		PAS_3	282..366	CDD:430001	24/106 (23%)
hif1ab	NP_001296971.1	bHLH_SF	12..75	CDD:469605	21/71 (30%)
		PAS	94..149	CDD:214512	19/54 (35%)
		PAS_3	254..339	CDD:430001	24/84 (29%)
		HIF-1	543..574	CDD:463274	8/48 (17%)
		HIF-1a_CTAD	740..776	CDD:430212