Protein Alignment Akt and PKN2

DIOPT Version :10

• Sequence 1: NP_732113.3
• Gene: Akt / 41957
• FlyBaseID: FBgn0010379
• Length: 611
• Species: Drosophila melanogaster
• Sequence 2: NP_006247.1
• Gene: PKN2 / 5586
• HGNCID: 9406
• Length: 984
• Species: Homo sapiens
• Alignment Length: 731
• Identity: 216/731 - (29%)
• Similarity: 317/731 - (43%)
• Gaps: 204/731 - (27%)

  Fly    43 YSQSTHPNSSPTSGSAEKFSWQQSWPSRTSAAPT---HDSGTMSINTTFDLSSPSVTSG------ 98
            ||.....|..|.:....:...::.  |..:|:||   ..|...:.|....||.|:..:|
Human   273 YSLEQRLNEVPKNHPKSRIIIEEL--SLVAASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVRL 335

  Fly    99 ----------HALTEQTQVVKEGW--------LMKRGEHIKNWRQRYF------------VLHSD 133
                      ...::.|.|...||        .|.|....|:...|..            ||..|
Human   336 MGCQDILENVPGRSKATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLD 400

  Fly   134 GRLMGYRS-KPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTT------------ 185
            ..::|..| ||..:.|....|.|.           :||.:.....:....|.:
Human   401 NTVVGQTSWKPISNQSWDQKFTLE-----------LDRSRELEISVYWRDWRSLCAVKFLRLEDF 454

  Fly   186 ---------------------------VIERTFAVESELERQQ---------------------- 201
                                       ||||    ..:|:||:
Human   455 LDNQRHGMCLYLEPQGTLFAEVTFFNPVIER----RPKLQRQKKIFSKQQGKTFLRAPQMNINIA 515

  Fly   202 -WTEAIR------NVSSRLIDVGEVAMT-------------PSEQTDMTDVDM------------ 234
             |...:|      |.|........|..|             |:..:.:|.:|.
Human   516 TWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASDSTVTKLDFDLEPEPPPAPPR 580

  Fly   235 -ATIAE-DELSE-------------QFSVQGTTCNS--------------------SGVKKV--- 261
             :::.| ||.||             .|.:|... ||                    ||::::
Human   581 ASSLGEIDESSELRVLDIPGQDSETVFDIQNDR-NSILPKSQSEYKPDTPQSGLEYSGIQELEDR 644

  Fly   262 --------TLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVL 318
                    .|::|....|||:|.||||:|...|.|.:::|||.|||..|:.:|||...:.|.|:.
Human   645 RSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIF 709

  Fly   319 KSTN---HPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYL 380
            ::.|   ||||::|...|||.:.:||||:|..||:|..|: |..:|:|.|..||.|.::..|.||
Human   710 ETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHI-HTDVFSEPRAVFYAACVVLGLQYL 773

  Fly   381 HSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAV 445
            |...|:||||||:|||||.:|.:|:||||||||.:.||..|.|||||||:||||||.:..|.:||
Human   774 HEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAV 838

  Fly   446 DWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGG 510
            ||||.||::|||:.|..||...|.:.:|..|:.:||::||.::.||.:::..||.::|::|||..
Human   839 DWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAS 903

  Fly   511 KDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPP-DPTGPLG 574
            :.|.::::.||||..|:|:.|:.||:.|||.|.:....|...||.|||.|:..|||| :|.  :.
Human   904 EKDAEDVKKHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPR--IL 966

  Fly   575 SIAEEPLFPQFSYQGD 590
            |..|:.:|..|.|..|
Human   967 SEEEQEMFRDFDYIAD 982

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Akt	NP_732113.3	PH_PKB	105..214	CDD:269947	32/197 (16%)
		STKc_PKB	270..590	CDD:270723	151/323 (47%)
PKN2	NP_006247.1	HR1_PKN_1	42..107	CDD:212012
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	114..133
		HR1	130..201	CDD:469609
		HR1_PKN2_3	209..282	CDD:212025	3/8 (38%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	351..383		8/31 (26%)
		Necessary to rescue apical junction formation. /evidence=ECO:0000250	382..463		13/91 (14%)
		C2_PKN-like	390..473	CDD:176069	13/93 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	558..584		2/25 (8%)
		STKc_PKN	657..982	CDD:270741	152/327 (46%)
		Necessary for the catalytic activity	917..977		24/61 (39%)
		Negatively regulates the responsiveness of the catalytic activity by cardiolipin and is required for optimal activation by the GTP-bound RhoA	978..984		2/5 (40%)