DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Akt1 and gad8

DIOPT Version :9

Sequence 1:	NP_732113.3	Gene:	Akt1 / 41957	FlyBaseID:	FBgn0010379	Length:	611	Species:	Drosophila melanogaster
Sequence 2:	NP_588010.1	Gene:	gad8 / 2539206	PomBaseID:	SPCC24B10.07	Length:	569	Species:	Schizosaccharomyces pombe

Alignment Length:	588	Identity:	209/588 - (35%)
Similarity:	302/588 - (51%)	Gaps:	93/588 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    50 NSSPTSGSAEKFSWQQSWPS----RTSAAPTHDSGTMSINTTFDLSSPSVTSGHALTEQTQVVKE 110
            ||:.:|.|:.|.|.::|..|    |:..:.....|.:.: |..:..:..:.|||........:..
pombe    33 NSTLSSTSSRKSSDEKSRKSSEDKRSPQSTVVQPGLLQV-TIIEARNLKLPSGHVPANYGVSIDN 96

  Fly   111 GWL---MKRGE-HIKN----WRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMT 167
            ..|   :..|. |.::    |...|.|:                     :|..|...|......:
pombe    97 SLLAPPLSNGSGHARSRSHAWWLPYIVV---------------------EFDKNEILVDALNTAS 140

  Fly   168 VDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLID-------VGEVAMTPSE 225
            ::.|          .|.  .:.||.| |...:......:|:.|||..:       :|.:.::||.
pombe   141 LENP----------CWD--YQATFDV-SRYSKLSLNIYLRSSSSRSRNGMGNDAFLGGIKLSPSF 192

  Fly   226 QTD-MTDVDMATIAEDELSEQFSVQGTTCNSSGVKKV------------TLENFEFLKVLGKGTF 277
            ..: :||            |...:.|    .||..:|            |::.||.|||:|||:|
pombe   193 IVNKLTD------------EWVPLHG----GSGELRVQMLYKPNQSTPLTIDAFELLKVVGKGSF 241

  Fly   278 GKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFV 342
            |||:..|::.|:::||:|.:||..|:.:.||.|||.|..||...|:||::.||:|||:..:|..|
pombe   242 GKVMQVRKRDTSRIYALKTMKKAHIVSRSEVDHTLAERTVLAQVNNPFIVPLKFSFQSPGKLYLV 306

  Fly   343 MQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVAD 407
            :.:|||||||.||..|..|...|.:||.||::.||..||...:||||||.||:|||..|||.:.|
pombe   307 LAFVNGGELFHHLQREGCFDTYRAKFYIAELLVALECLHEFNVIYRDLKPENILLDYTGHIALCD 371

  Fly   408 FGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVL 472
            |||||.::.....|.||||||||||||:|..:.|.:.||||..||::||||.|..|||:.:.:.:
pombe   372 FGLCKLNMAKTDRTNTFCGTPEYLAPELLLGHGYTKVVDWWTLGVLLYEMITGLPPFYDENINEM 436

  Fly   473 FTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIP 537
            :..||.:.::||.||.::||:||:|||.:.|:||||.|  ..:||:.||||..|:|..|..|||.
pombe   437 YRKILQDPLRFPDNIDEKAKDLLSGLLTRAPEKRLGSG--GAQEIKNHPFFDDIDWKKLCAKKIQ 499

  Fly   538 PPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAE--EPLFPQFSYQGDMASTLGTSSH 600
            |||||.|.|..||..||.|||.|    .|.|.......::|  :..|..:|||  ..:|:.||..
pombe   500 PPFKPSVESAIDTSNFDSEFTSE----IPMDSVVADSHLSETVQQRFANWSYQ--RPTTIDTSDD 558

  Fly   601 IST 603
            |:|
pombe   559 INT 561

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Akt1	NP_732113.3	PH_PKB	105..214	CDD:269947	19/116 (16%)
		PH	107..211	CDD:278594	16/111 (14%)
		S_TKc	266..523	CDD:214567	130/256 (51%)
		STKc_PKB	270..590	CDD:270723	157/321 (49%)
gad8	NP_588010.1	YPK1_N_like	66..225	CDD:212165	34/209 (16%)
		PTZ00263	214..545	CDD:140289	158/336 (47%)
		STKc_YPK1_like	235..547	CDD:270737	153/317 (48%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	316	1.000	Inparanoid score	I558
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100157
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R469
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.890