Protein Alignment Hel89B and RAD16

DIOPT Version :10

• Sequence 1: NP_732097.2
• Gene: Hel89B / 41943
• FlyBaseID: FBgn0022787
• Length: 1923
• Species: Drosophila melanogaster
• Sequence 2: NP_009672.1
• Gene: RAD16 / 852411
• SGDID: S000000318
• Length: 790
• Species: Saccharomyces cerevisiae
• Alignment Length: 754
• Identity: 174/754 - (23%)
• Similarity: 302/754 - (40%)
• Gaps: 195/754 - (25%)

  Fly  1274 VVPLLGAMSDPDESVRLLSTHCFAN----LVQLMPLDGKTEQLKSD---PL-QARKTRDRE---- 1326
            ::.|.|  ||.:.:....:||...|    ::::     |.|:..||   || :.|||..|:
Yeast    72 IIDLTG--SDKERTATDENTHAIKNDNDEIIEI-----KEERDVSDDDEPLTKKRKTTARKKKKK 129

  Fly  1327 ------------------------------FLDYLFNPKSIP-NYKVPVPISVELRCYQQAGINW 1360
                                          |.|....|..:| ..|.|..::::|..:|..|::|
Yeast   130 TSTKKKSPKVTPYERNTLRLYEHHPELRNVFTDLKNAPPYVPQRSKQPDGMTIKLLPFQLEGLHW 194

  Fly  1361 LWFLNKYNLH--GILCDDMGLGKTLQTICILAGDHMHRQTANLANLPSLVICPPTLTGHWVYEVE 1423
            | ...:.:::  |:|.|:||:|||:|||.:|..|        |...||||:.|......|..|:|
Yeast   195 L-ISQEESIYAGGVLADEMGMGKTIQTIALLMND--------LTKSPSLVVAPTVALMQWKNEIE 250

  Fly  1424 K--------FLDQGSV----LRPLHYYGFPVGREKLRSDIGTKCNLVVASYDTVRKDIDFFSGIH 1476
            :        ::..|:.    ::.|..|...:....:...:..|.|......:.:.|.......|.
Yeast   251 QHTKGQLKIYIYHGASRTTDIKDLQGYDVVLTTYAVLESVFRKQNYGFRRKNGLFKQPSVLHNID 315

  Fly  1477 FNYCVLDEGHIIKNGKTKSSKAIKRLKANHRLILSGTPIQNNVLELWSLFDFL------------ 1529
            |...:|||.|.||:.::.:::|:..||...|..|||||:||.:.|::||..||
Yeast   316 FYRVILDEAHNIKDRQSNTARAVNNLKTQKRWCLSGTPLQNRIGEMYSLIRFLNINPFTKYFCTK 380

  Fly  1530 ----MPGFLGTEKQFVQRFSRPILSSRD------AKSSAK--EQEAGVLAMEALHRQVLPFLLRR 1582
                ...:..|::......|..|:...:      .|:..|  .:..|:.:...:...:...:|||
Yeast   381 CDCASKDWKFTDRMHCDHCSHVIMQHTNFFNHFMLKNIQKFGVEGPGLESFNNIQTLLKNIMLRR 445

  Fly  1583 VKEDVLKD--LPPKITQDLLCELSPLQLRLYEDFSNKHLKDCLDKLGDSSSA---SMVTEN--LS 1640
            .|.:...|  |||:|            :.:..||.|:..||....|...|..   |.|.|.  |:
Yeast   446 TKVERADDLGLPPRI------------VTVRRDFFNEEEKDLYRSLYTDSKRKYNSFVEEGVVLN 498

  Fly  1641 AKTHIFQALRYLQNVCNHPKLVLRQ-----SEELTKVTSQLA----------------------- 1677
            ...:||..:..::.:.:||.|||::     .:::..|..||.
Yeast   499 NYANIFTLITRMRQLADHPDLVLKRLNNFPGDDIGVVICQLCNDEAEEPIESKCHHKFCRLCIKE 563

  Fly  1678 ------------------------LSNSSLD-DI------------------EHSAKLPALKQLL 1699
                                    ||..:|: |:                  :.|.|:.||.:.|
Yeast   564 YVESFMENNNKLTCPVCHIGLSIDLSQPALEVDLDSFKKQSIVSRLNMSGKWQSSTKIEALVEEL 628

  Fly  1700 LDCGIGVQTESVSQHRALIFCQLKAMLDIVEQDLLRRHLPSVTYLRLDGSVPASQRQDIVNNFNS 1764
            .......:|     .::::|.|..:|||:||..|.|....:|   :|.||:..:||.:.:..|.:
Yeast   629 YKLRSNKRT-----IKSIVFSQFTSMLDLVEWRLKRAGFQTV---KLQGSMSPTQRDETIKYFMN 685

  Fly  1765 DPSIDVLLLTTMVGGLGLNLTGADTVIFVEHDWNPMKDLQAMDRAHRIGQKKVVNVYRLITRNSL 1829
            :...:|.|::...||:.|||..|..|..::..|||..:.|:.||.|||||.:.|.:.|....:|:
Yeast   686 NIQCEVFLVSLKAGGVALNLCEASQVFILDPWWNPSVEWQSGDRVHRIGQYRPVKITRFCIEDSI 750

  Fly  1830 EEKIMGLQKFKILTANTVVSAENASLQTMGTSQIFDLFN 1868
            |.:|:.||:.|....:..::.:.|::..:..:.:..|||
Yeast   751 EARIIELQEKKANMIHATINQDEAAISRLTPADLQFLFN 789

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hel89B	NP_732097.2	HEAT repeat	5..30	CDD:293787
		HEAT repeat	42..70	CDD:293787
		HEAT repeat	395..420	CDD:293787
		HEAT repeat	433..465	CDD:293787
		HEAT repeat	475..504	CDD:293787
		HEAT repeat	516..540	CDD:293787
		HEAT repeat	560..589	CDD:293787
		DUF3535	654..1138	CDD:463447
		HepA	<1342..1849	CDD:440319	148/622 (24%)
RAD16	NP_009672.1	HepA	<172..769	CDD:440319	149/625 (24%)
		RING-HC_RAD16-like	536..584	CDD:438229	2/47 (4%)