Protein Alignment Hel89B and RAD5

DIOPT Version :10

• Sequence 1: NP_732097.2
• Gene: Hel89B / 41943
• FlyBaseID: FBgn0022787
• Length: 1923
• Species: Drosophila melanogaster
• Sequence 2: NP_013132.1
• Gene: RAD5 / 850719
• SGDID: S000004022
• Length: 1169
• Species: Saccharomyces cerevisiae
• Alignment Length: 862
• Identity: 200/862 - (23%)
• Similarity: 309/862 - (35%)
• Gaps: 315/862 - (36%)

  Fly  1235 LLPLLGK--IEQLIERQGAIEAIERVVSRLQIKVVPYIVLLVVPLLGAMSDPDESVRLLSTHCFA 1297
            ||.|..|  ::.:::.|.|:|.     .::::...|.|:.|         |.||......|....
Yeast   340 LLKLFDKLRLKPILDEQKALEK-----HKIELNSDPEIIDL---------DNDEICSNQVTEVHN 390

  Fly  1298 NL------VQLMPLDGKTEQLKSDPLQARKTRDREFLDYLFNPKSIPNYKVPVPISVELRCYQQA 1356
            ||      .:.|.|:    |||:....|:.:...:.|     |::.|:..|   ..:|||.||:.
Yeast   391 NLRDTQHEEETMNLN----QLKTFYKAAQSSESLKSL-----PETEPSRDV---FKLELRNYQKQ 443

  Fly  1357 GINW---------------------------LWFLNKY--------------------------N 1368
            |:.|                           ||...|:                          |
Yeast   444 GLTWMLRREQEFAKAASDGEASETGANMINPLWKQFKWPNDMSWAAQNLQQDHVNVEDGIFFYAN 508

  Fly  1369 LH-----------------GILCDDMGLGKTLQTI-CILAGDHMH----------RQTANLANLP 1405
            ||                 |||.|:||||||:... .:|:..|..          ..||...|||
Yeast   509 LHSGEFSLAKPILKTMIKGGILSDEMGLGKTVAAYSLVLSCPHDSDVVDKKLFDIENTAVSDNLP 573

  Fly  1406 S--------------LVICPPTLTGHWVYEVEKFLDQGSVLRPLHYYGFPVGREKLRSDIGTKCN 1456
            |              |::.|.:|...|..|..|..:...:...::|.|.....:.|.:...|...
Yeast   574 STWQDNKKPYASKTTLIVVPMSLLTQWSNEFTKANNSPDMYHEVYYGGNVSSLKTLLTKTKTPPT 638

  Fly  1457 LVVASYDTVR-------------KDIDFFSG---IHFNYCVLDEGHIIKNGKTKSSKAIKRLKAN 1505
            :|:.:|..|:             :|::..||   ::|...::||||.|:|..|.:|||:..|:..
Yeast   639 VVLTTYGIVQNEWTKHSKGRMTDEDVNISSGLFSVNFYRIIIDEGHNIRNRTTVTSKAVMALQGK 703

  Fly  1506 HRLILSGTPIQNNVLELWSLFDFL----------MPGFLGT---EKQFVQRFS------RPILSS 1551
            .:.:|:||||.|.:.:|:||..||          ...|:.|   .|.:.|.|.      .|:|..
Yeast   704 CKWVLTGTPIINRLDDLYSLVKFLELDPWRQINYWKTFVSTPFESKNYKQAFDVVNAILEPVLLR 768

  Fly  1552 RDAKSSAKEQEAGVLAMEALHRQVLPFLLRRVKEDVLKDLPPKITQDLLCELSPLQLRLYEDFSN 1616
            |..:...|:.:              |.:....||.|:|.||...:||||
Yeast   769 RTKQMKDKDGK--------------PLVELPPKEVVIKRLPFSKSQDLL---------------- 803

  Fly  1617 KHLKDCLDKLGDSSSASMVTENLSAK--THIFQALRYLQNVCNHP-----------------KLV 1662
              .|..|||...|..:.:...:|..|  |.:...|| |:.||.||                 |||
Yeast   804 --YKFLLDKAEVSVKSGIARGDLLKKYSTILVHILR-LRQVCCHPGLIGSQDENDEDLSKNNKLV 865

  Fly  1663 LRQS--------------------EELTKVTSQLAL---SNSSLDDIEHS----AKLPALKQLLL 1700
            ..|:                    |||..:..:|.:   .|.|...:|.|    ..:...|.|..
Yeast   866 TEQTVELDSLMRVVSERFDNSFSKEELDAMIQRLKVKYPDNKSFQSLECSICTTEPMDLDKALFT 930

  Fly  1701 DCG-------------------IG------------------VQTESVSQH-------------- 1714
            :||                   :|                  |||.|.|::
Yeast   931 ECGHSFCEKCLFEYIEFQNSKNLGLKCPNCRNQIDACRLLALVQTNSNSKNLEFKPYSPASKSSK 995

  Fly  1715 ------------------RALIFCQLKAMLDIVEQDLLRRHLPSVTYL-RLDGSVPASQRQDIVN 1760
                              :.:||.|....|||:|::|.......|..: :.||.:...:|..::.
Yeast   996 ITALLKELQLLQDSSAGEQVVIFSQFSTYLDILEKELTHTFSKDVAKIYKFDGRLSLKERTSVLA 1060

  Fly  1761 NFN-SDPSID-VLLLTTMVGGLGLNLTGADTVIFVEHDWNPMKDLQAMDRAHRIGQKKVVNVYRL 1823
            :|. .|.|.. :|||:...||:|||||.|.....::..|:|..:.||:||.|||||...|.|.|.
Yeast  1061 DFAVKDYSRQKILLLSLKAGGVGLNLTCASHAYMMDPWWSPSMEDQAIDRLHRIGQTNSVKVMRF 1125

  Fly  1824 ITRNSLEEKIMGLQKFK 1840
            |.::|:|||::.:|:.|
Yeast  1126 IIQDSIEEKMLRIQEKK 1142

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hel89B	NP_732097.2	HEAT repeat	5..30	CDD:293787
		HEAT repeat	42..70	CDD:293787
		HEAT repeat	395..420	CDD:293787
		HEAT repeat	433..465	CDD:293787
		HEAT repeat	475..504	CDD:293787
		HEAT repeat	516..540	CDD:293787
		HEAT repeat	560..589	CDD:293787
		DUF3535	654..1138	CDD:463447
		HepA	<1342..1849	CDD:440319	175/747 (23%)
RAD5	NP_013132.1	HIRAN	176..284	CDD:400928
		HepA	<527..1143	CDD:440319	161/649 (25%)
		RING-HC_RAD5	909..973	CDD:438493	7/63 (11%)