DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Hel89B and RGD3

DIOPT Version :9

Sequence 1:	NP_001287351.1	Gene:	Hel89B / 41943	FlyBaseID:	FBgn0022787	Length:	1923	Species:	Drosophila melanogaster
Sequence 2:	NP_001190085.1	Gene:	RGD3 / 824595	AraportID:	AT3G54280	Length:	2129	Species:	Arabidopsis thaliana

Alignment Length:	2238	Identity:	764/2238 - (34%)
Similarity:	1088/2238 - (48%)	Gaps:	487/2238 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 TSRLDRLFILLETGSTAATRQAAAKQIGEVQKLYPHELHALLNRLVGYLHSTSWETRIAAAQTVE 66
            :|||:||..||:||||.|||..||||||::.|.:|.:|.:||.:::.:|.|..|:||:|||..:.
plant     6 SSRLNRLLTLLDTGSTQATRLTAAKQIGDIAKSHPQDLSSLLRKVLHHLRSKKWDTRVAAAHAIG 70

  Fly    67 AILKQVPPWQPEFQVLPKKERCATNTATAAAAAEEDSCQSSGSNSTTASERLLS-------FDEF 124
            ||            ||..|....:....:.|....::..|...:...|...|.|       |..|
plant    71 AI------------VLNVKHPSLSELLNSLATKLGEAGISDNVDEVVAFRNLQSKILANAPFRSF 123

  Fly   125 DLQQILHKGARLIGSEGNEFDVQ----EEQPAS-------------GGGEED------RSLASRL 166
            ::.::|..|| |:.|.|..|..:    :.||..             .|.|.|      ::...|:
plant   124 EMNKVLEFGA-LLASGGQAFITKNILCQLQPVKLFPTVSLFDLKKFYGLEYDILNDNSKNPRDRV 187

  Fly   167 SRQRAVLNDKLGLTTAAKLGVNLTDMITDED-------VALNRSGSTYNVNAEKLPVEHLLNIKT 224
            :||:..|..:|||....:. :::.:||.|||       |..|..|:....|.....::..:: :.
plant   188 ARQKKNLRRRLGLDMCEQF-MDVNEMIRDEDLIEQKSNVPANGVGNRLYANCSPHHIQQFVS-RM 250

  Fly   225 AANGSGQAPLSCREMNRAKRKAR-----QNTSGCSVTAVTPTCSRRNSNSNHSTGSNHSSNGTTG 284
            ....:.:.| |.||:|..||||:     |....|.|..|..:.|...|.|......:..|:
plant   251 VPRVNSRRP-SARELNLLKRKAKISSKDQAKGSCEVADVEMSSSHVASTSKRILSDSLDSS---- 310

  Fly   285 AAIEEPEKKKLKSTDRQEIFYSLNAAVPDATGTWVDAVSWPLENFCSRLFIDLFHAKWEVRHGAA 349
                   |..:.:.|..|         ||..|      .||..:|..:|.:|:|...||:|||:.
plant   311 -------KADIGNEDDIE---------PDGDG------KWPFHSFVEQLILDMFDPAWEIRHGSV 353

  Fly   350 TALRELINQHAQGAG--------------KAVLQ--TREQ------------------------- 373
            .||||::..|...||              |.||.  |||:
plant   354 MALREILMLHGGSAGVSTEEFSSDNGFELKDVLNKVTREREIDLNMQVSENELEPLRKRPKIEDP 418

  Fly   374 ---------------------------------------------------MDE--AHSR----- 380
                                                               ||:  :||.
plant   419 SKSFIDNTVLEVIGGDYDINVKDEDAEFLLPPVKVNGQTDCSSTKLEPQSSMDDSTSHSEINHVA 483

  Fly   381 -------------------------------------W------LEDAALRLLCVLCLDRFGDFV 402
                                                 |      |:|..:|.||||.||||||::
plant   484 EVNNHFEDKSFIEEPVIPKQQEENLEVLDLVKQARHSWIKNFEFLQDCTIRFLCVLSLDRFGDYI 548

  Fly   403 SDQVVAPVRETCAQVLGTLVKEMEASKVQRIVNILLQLIQHKNEWDVRHGGLLGLKYVFVVREEL 467
            ||||||||||.|||.||...|.|..|.:...:|||||: |.:.||::|||.|||:||:..||:|:
plant   549 SDQVVAPVREACAQALGATFKYMNPSLIYETLNILLQM-QRRPEWEIRHGSLLGIKYLVAVRQEM 612

  Fly   468 LPIYLPQSISNILIGLLDAVDDVGAVAAATLIPVSSWLPKLLNPAQVSSIVKMLWDLLLDQDELT 532
            |...|...:.....||.|:.|||.||||..|||.::.:..|.... :.|||.:|||:||:.|:|:
plant   613 LQDLLGYILPACKAGLEDSDDDVRAVAADALIPAAAAIVSLRGQT-LLSIVMLLWDILLELDDLS 676

  Fly   533 SACNSFMGLLAAILCLPNAA-----------------------------RWVEMEPMAI--LVPR 566
            .:.:|.|.|||.|....:..                             |.|:..|.|:  |.||
plant   677 PSTSSIMNLLAEIYSQDDMTLVMHEELSLGEEQNIELNEMGHIESIGERRDVKESPYALSGLAPR 741

  Fly   567 LWPFLSHSTSSVRRSTLKTLMTLTSA---DRVKSEPKDENQVKEEADKEEMKLNFGVSDWKWELL 628
            ||||..|..:|||.|.::||..|..|   ..:..:.|.                   |.|...:|
plant   742 LWPFTRHDITSVRFSAIRTLERLLEAGCRKNISGQSKS-------------------SFWPSSIL 787

  Fly   629 QQALRHIYQRILVEPQADIQALARQVWSNLIQHADLGALLHAACP----------FVSSWICLSM 683
            ...||.::|.:|:|...:|...:.:||..|:|           ||          :::|||.|:.
plant   788 GDTLRIVFQNLLLESTEEILECSERVWRLLVQ-----------CPVDDLEDTAKFYMASWIELAA 841

  Fly   684 QPPRLAFDAGVLIRAGGDVSTPGSTSRKRTPRI--------GDELGGNILAQSNASL-KLYLGGS 739
            .|.....||..:.   ..|:.|..:..|...::        ...:.|...|:|:||| |.....:
plant   842 TPYGSTLDATKMF---WPVAPPRKSHFKAAAKMKAVKLENEASSILGFDYARSSASLEKQEDASA 903

  Fly   740 EATPLDV---RDANFMRARISSSRALGALSHYLVQPAPGVVYTPQTESPTDCYTKVLLGHLNAHS 801
            .:|.:.|   .:.:..|.|:.::.|||..:..|.:.:...|..|            |...|.:.|
plant   904 RSTKIIVGSDMEMSVTRTRVVTASALGIFASRLREGSMQFVVDP------------LSSTLTSMS 956

  Fly   802 AVQRIVCGLIIAFWALEDE--------PVRPGPPNLQEK----LHQCVSE-------YVYYDEVA 847
            .|||.|..:::..|..|.:        ...||.|:..:|    |..|...       ::.|.|::
plant   957 GVQRQVGSIVLISWFRETKCKAPSDGSGSLPGFPSPLKKWLLDLLACADPAFPTKDIFLPYAELS 1021

  Fly   848 ISLTRLLQEAQDFIAT----------LKQNKIPINDFNNAKILTLDQIEAVATTL----SENLRS 898
            .:.|::..||...:.|          |..||:      |.:.:|.|:....|:||    .|:..:
plant  1022 RTYTKMRNEASQLLHTVETCHCFDKLLSTNKL------NVESVTADETIDFASTLDLWNKESAGN 1080

  Fly   899 YPLKPKVLDMLEERRRSLQASYQQTTSEQSAYHVSAQAALAGAICALHCLPDKLNPVVKPLMESI 963
            ..|:.:|.:.:|..|:.|.::.......||..|::..:.:|.|:..:...|.:|||::.|||.||
plant  1081 ESLEKQVFEDVESSRQQLLSTAGYLKCVQSNLHITVTSLVAAAVVWMSEFPARLNPIILPLMASI 1145

  Fly   964 KREQCLQLQQLSAEFLVHLMDQVCDRNPSPNSKILNNLCTLLRSDPEFTPKLVMPLETLKQTPVS 1028
            ||||...|||::||.|..|:....||.||||.|::.|:|:|...||..||:..:         :|
plant  1146 KREQEQILQQIAAEALAELIAYCVDRKPSPNDKLIKNICSLTCMDPSETPQASI---------IS 1201

  Fly  1029 S-EVINNCVYYGILTLALQQTVTTTNTRSGAGGATTPTATTAPRGPGRPPTGEILSATNATAHIE 1092
            | :::::.                                            :.||:.:.|...:
plant  1202 SMDIVDDM--------------------------------------------DFLSSRSNTGKQK 1222

  Fly  1093 LKAQQAKEAEAKQCR--IQRLGAACAIEKLCRIFGEQIIEKVAVFQHLMFGKVEQFVKE------ 1149
            .|...|...:..:..  |.|.|:..|::.|...||..:.:|:......:   .|..|.|
plant  1223 AKVVLASGEDRSKVEGFITRRGSELALKHLSLKFGGSLFDKLPKLWECL---TEVLVPEIPSDQQ 1284

  Fly  1150 AYDWRLGSLL-PDLGVCNELVSSMQLIETAAPHLHVALHPQMFALLPSLGFIVCHPLKAVRHMAA 1213
            ..|.::.|:. |.:     |::::|::.:.||.:...|.|::.:|||.:...|.|...|||..|:
plant  1285 KIDLKIESISDPQV-----LINNIQVVRSIAPVMEETLKPRLLSLLPCIFKCVRHSHVAVRLAAS 1344

  Fly  1214 RCIAVLAEIDACQTMQFVVHDLLPLLGKIEQLIERQGAIEAIERVVSRLQIKVVPYIVLLVVPLL 1278
            ||:..:|:......|..||...:|:||.:..:..||||...|..:|..|.:::|||..|||||||
plant  1345 RCVMTMAKSMTTDVMAAVVESAIPMLGDLTCISGRQGAGMLIGLLVQGLGVELVPYSPLLVVPLL 1409

  Fly  1279 GAMSDPDESVRLLSTHCFANLVQLMPLD-------GKTEQLKSDPLQARKTRDREFLDYLFNPKS 1336
            ..|||.|.|||...|..||.||.::||.       |.::.|.|:      ..|.:||:.|.:...
plant  1410 RCMSDVDSSVRQSVTRSFAALVPMLPLARGVPPPVGLSKDLSSN------AEDAKFLEQLLDNSH 1468

  Fly  1337 IPNYKVPVPISVELRCYQQAGINWLWFLNKYNLHGILCDDMGLGKTLQTICILAGDHMHRQ--TA 1399
            |.:||:...:.|:||.|||.|||||.||.::.||||||||||||||||...|:|.|...|:  |.
plant  1469 IDDYKLCTELKVQLRRYQQEGINWLGFLKRFKLHGILCDDMGLGKTLQASAIVASDAAERRGSTD 1533

  Fly  1400 NLANLPSLVICPPTLTGHWVYEVEKFLDQGSVLRPLHYYGFPVGREKLRSDIGTKCNLVVASYDT 1464
            .|...||:::||.||.|||.:|:||::|. |:|..|.|.|....|..||...... |:::.|||.
plant  1534 ELDVFPSIIVCPSTLVGHWAFEIEKYIDL-SLLSVLQYVGSAQDRVSLREQFNNH-NVIITSYDV 1596

  Fly  1465 VRKDIDFFSGIHFNYCVLDEGHIIKNGKTKSSKAIKRLKANHRLILSGTPIQNNVLELWSLFDFL 1529
            ||||:|:.:...:|||:|||||||||.|:|.:.|:|:|||.|||||||||||||::|||||||||
plant  1597 VRKDVDYLTQFSWNYCILDEGHIIKNAKSKITAAVKQLKAQHRLILSGTPIQNNIMELWSLFDFL 1661

  Fly  1530 MPGFLGTEKQFVQRFSRPILSSRDAKSSAKEQEAGVLAMEALHRQVLPFLLRRVKEDVLKDLPPK 1594
            ||||||||:||...:.:|:|::||.|.|||:.|||||||||||:||:||||||.||:||.|||.|
plant  1662 MPGFLGTERQFQASYGKPLLAARDPKCSAKDAEAGVLAMEALHKQVMPFLLRRTKEEVLSDLPEK 1726

  Fly  1595 ITQDLLCELSPLQLRLYEDFSNKHLKDCLDKL----GDSSSASMVTENLSAKTHIFQALRYLQNV 1655
            |.||..|:|||:||:|||.||....|..:..:    |.:.|.:.......|.||:||||:||..:
plant  1727 IIQDRYCDLSPVQLKLYEQFSGSSAKQEISSIIKVDGSADSGNADVAPTKASTHVFQALQYLLKL 1791

  Fly  1656 CNHPKLVLRQSEELTK-VTSQLALSNSSLDDI-------EHSAKLPALKQLLLDCGIGVQ----- 1707
            |:||.|||  .:::|: |.|.||...:...||       :||.||.||:::|.:||||..
plant  1792 CSHPLLVL--GDKVTEPVASDLAAMINGCSDIITELHKVQHSPKLVALQEILEECGIGSDASSSD 1854

  Fly  1708 -TESVSQHRALIFCQLKAMLDIVEQDLLRRHLPSVTYLRLDGSVPASQRQDIVNNFNSDPSIDVL 1771
             |.||.|||.|||.|.||:|||:|:||.:.|:.||||:||||||...:|.:||..|||||:||||
plant  1855 GTLSVGQHRVLIFAQHKALLDIIEKDLFQAHMKSVTYMRLDGSVVPEKRFEIVKAFNSDPTIDVL 1919

  Fly  1772 LLTTMVGGLGLNLTGADTVIFVEHDWNPMKDLQAMDRAHRIGQKKVVNVYRLITRNSLEEKIMGL 1836
            ||||.|||||||||.|||::|:|||||||:|.||||||||:|||:||||:|||.|.:||||:|.|
plant  1920 LLTTHVGGLGLNLTSADTLVFMEHDWNPMRDHQAMDRAHRLGQKRVVNVHRLIMRGTLEEKVMSL 1984

  Fly  1837 QKFKILTANTVVSAENASLQTMGTSQIFDLF--------NGGKDKGAESGSSAVQGTASGGMSMN 1893
            ||||:..||||::|||||::||.|.|:.|||        .||..|.....:..:.||..|   |.
plant  1985 QKFKVSVANTVINAENASMKTMNTDQLLDLFASAETSKKGGGSSKKGSEDNDQIAGTGKG---MK 2046

  Fly  1894 TIIENLPELWSEHQYEEEYDLGNFVQAL 1921
            .|:.||.|||.:.||.|||:|..|:..|
plant  2047 AILGNLEELWDQSQYTEEYNLSQFLTKL 2074

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hel89B	NP_001287351.1	HEAT repeat	5..30	CDD:293787	17/24 (71%)
		HEAT repeat	42..70	CDD:293787	12/27 (44%)
		HEAT repeat	395..420	CDD:293787	20/24 (83%)
		HEAT repeat	433..465	CDD:293787	17/31 (55%)
		HEAT repeat	475..504	CDD:293787	13/28 (46%)
		HEAT repeat	516..540	CDD:293787	11/23 (48%)
		HEAT repeat	560..589	CDD:293787	15/30 (50%)
		DUF3535	654..1138	CDD:288874	123/541 (23%)
		HEAT repeat	1234..1261	CDD:293787	9/26 (35%)
		HEAT repeat	1269..1298	CDD:293787	17/28 (61%)
		SNF2_N	1353..1662	CDD:278600	177/314 (56%)
		DEXDc	1370..1514	CDD:238005	77/145 (53%)
		Helicase_C	1689..1813	CDD:278689	84/129 (65%)
RGD3	NP_001190085.1	HEAT repeat	9..34	CDD:293787	17/24 (71%)
		HEAT repeat	46..73	CDD:293787	12/38 (32%)
		HEAT repeat	541..566	CDD:293787	20/24 (83%)
		HEAT repeat	579..607	CDD:293787	16/28 (57%)
		HEAT_EZ	592..637	CDD:290247	20/44 (45%)
		HEAT repeat	619..668	CDD:293787	19/49 (39%)
		HEAT repeat	677..724	CDD:293787	6/46 (13%)
		HEAT repeat	735..764	CDD:293787	14/28 (50%)
		DUF3535	813..1270	CDD:288874	123/541 (23%)
		SNF2_N	1485..1798	CDD:278600	177/314 (56%)
		DEXDc	1502..1646	CDD:238005	77/145 (53%)
		HELICc	1822..1966	CDD:238034	88/143 (62%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	336	1.000	Domainoid score	I249
eggNOG	1	0.900	-	-		E1_KOG0392
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H31978
Inparanoid	1	1.050	1086	1.000	Inparanoid score	I30
OMA	1	1.010	-	-		QHG55547
OrthoDB	1	1.010	-	-		D61251at2759
OrthoFinder	1	1.000	-	-		FOG0004898
OrthoInspector	1	1.000	-	-		oto3673
orthoMCL	1	0.900	-	-		OOG6_101665
Panther	1	1.100	-	-	LDO	PTHR10799
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3453
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.840