Protein Alignment Rbf2 and RB1

DIOPT Version :10

• Sequence 1: NP_524372.1
• Gene: Rbf2 / 41941
• FlyBaseID: FBgn0038390
• Length: 783
• Species: Drosophila melanogaster
• Sequence 2: NP_000312.2
• Gene: RB1 / 5925
• HGNCID: 9884
• Length: 928
• Species: Homo sapiens
• Alignment Length: 893
• Identity: 186/893 - (20%)
• Similarity: 348/893 - (38%)
• Gaps: 262/893 - (29%)

  Fly    11 ETLVRRFSVSCEQLELEARIQQSALSTYHRLDAVNGL--STSEADAQEWLCCAVYSELQRSKMRD 73
            ||....|:..|::|::...:::.|..|:.::.:|:|:  ...:...:.|..|...:.:...:|
Human    51 ETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEM-- 113

  Fly    74 IRESINEANDSVAKNCCWNVSLTRLLRSFKMNVSQFLRRMEHWNWLTQNENTFQLEVEELRCRLG 138
                              :.:.|.|.::.:::|.:|...::..:..|:.:|..
Human   114 ------------------SFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAM------------ 148

  Fly   139 ITSTLLRHYKHIFRSLFVHPGKGAD------PGAANHYQ----ALYEFGWLLFLVIRNELPGFAI 193
              |.||:.|..:| :||....:..:      |.::...:    .:.:..|:.||:.:.|:....
Human   149 --SRLLKKYDVLF-ALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQME- 209

  Fly   194 TNLINGCQVLVCTMDLLFVNALEVPRSVVIRREFSGVPKNWDTEDFNPILLN---KYSVLEALGE 255
            .:|:...|:::|.:| .|:....                        |:||.   |.:|:...|.
Human   210 DDLVISFQLMLCVLD-YFIKLSP------------------------PMLLKEPYKTAVIPINGS 249

  Fly   256 LIPELPAKGVVQMKNAFFHKALIMLYMDHSLVGDDTHMREII-KE-------------------- 299
              |..|.:|  |.::|...|.|          .:||.:.|:: ||
Human   250 --PRTPRRG--QNRSARIAKQL----------ENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFM 300

  Fly   300 ---GMLDIN----LENLNRKYTNQVADISEMDERVLLSVQGAIETKGDSPKSPQLAFQTSSSPSH 357
               |::..|    :|||:::|........::|.|:.|.....::|  ||..|    |:|..:|..
Human   301 NSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQT--DSIDS----FETQRTPRK 359

  Fly   358 RKL--STHDLPASLP----------LSIIKAFPKKEDADKIVNYLDQTLEEMNRTFTMAVKD--- 407
            ..|  ..:.:|...|          |.:|......:.::.:::|.:........:....|||
Human   360 SNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGY 424

  Fly   408 -----FLDA------ELSGKRFRQARGLYYKYLQKILGPELVQKPQLKIGQLMKQRKLTAALLAC 461
                 |..|      |:..:|::....|||:.::.:|..|..:.......:|:.......:||||
Human   425 IFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLAC 489

  Fly   462 CLELALHVHHK-----LVEG--LRFPFVLHCFSLDAYDFQKILELVVRYDHGFLGRELIKHLDVV 519
            .||:.:..:.:     |..|  |.||::|:..:|.|:||.|::|..::.: |.|.||:||||:..
Human   490 ALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAE-GNLTREMIKHLERC 553

  Fly   520 EEMCLDSLIFRKSSQLWWELNQ-----------------------------------RLPRYK-- 547
            |...::||.:...|.|:..:.|                                   |.|:.|
Human   554 EHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGS 618

  Fly   548 ------EVDAETEDKENF-------STGSSICLRKFYGLANRRLLLLCKSLCLVDSFPQ----IW 595
                  ..:|||:....|       ||..|:..:|.|.||..||..||:.  |:...|:    ||
Human   619 TTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCER--LLSEHPELEHIIW 681

  Fly   596 HLAEHSFTLESS-RLLRNRHLDQLLLCAIHLHVRLEKLHLTFSMIIQHYRRQPHFRRSAYREVSL 659
            .|.:|  ||::. .|:|:|||||:::|:::...:::.:.|.|.:|:..|:..||..:..::.|.:
Human   682 TLFQH--TLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLI 744

  Fly   660 GNGQTADIITFYNSVYVQSMGNYGRHLECAQTRKSLEESQSNVGILTETTSNELSMRANISISSP 724
            ...:...||.|||||::|.:                   ::|:                :..:|.
Human   745 KEEEYDSIIVFYNSVFMQRL-------------------KTNI----------------LQYAST 774

  Fly   725 PPPRVCQSGSCSSHPPSSPAASPLSLQSSPNVKRAASSNDLMREIKRP 772
            .||.:    |...|.|.||...|    |||  .|....|..:..:|.|
Human   775 RPPTL----SPIPHIPRSPYKFP----SSP--LRIPGGNIYISPLKSP 812

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rbf2	NP_524372.1	DUF3452	83..213	CDD:463402	23/139 (17%)
		RB_A	<384..536	CDD:460364	44/172 (26%)
		RB_B	<593..679	CDD:460363	29/90 (32%)
RB1	NP_000312.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..42
		DUF3452	114..227	CDD:463402	22/129 (17%)
		Pocket, binds T and E1A	373..771		99/437 (23%)
		Domain A	373..579		49/206 (24%)
		RB_A	373..573	CDD:460364	48/200 (24%)
		Spacer	580..639		7/58 (12%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	610..632		6/21 (29%)
		Domain B	640..771		43/169 (25%)
		CYCLIN_RB	645..771	CDD:410302	42/164 (26%)
		Interaction with LIMD1. /evidence=ECO:0000269|PubMed:15542589	763..928		17/95 (18%)
		Rb_C	768..925	CDD:462639	16/71 (23%)
		Domain C, mediates interaction with E4F1. /evidence=ECO:0000269|PubMed:10869426	771..928		16/52 (31%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	860..928
		Bipartite nuclear localization signal. /evidence=ECO:0000250|UniProtKB:P13405	860..876