DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment spn-E and Dhx36

DIOPT Version :9

Sequence 1:	NP_476741.1	Gene:	spn-E / 41919	FlyBaseID:	FBgn0003483	Length:	1434	Species:	Drosophila melanogaster
Sequence 2:	NP_082412.2	Gene:	Dhx36 / 72162	MGIID:	1919412	Length:	1001	Species:	Mus musculus

Alignment Length:	886	Identity:	256/886 - (28%)
Similarity:	403/886 - (45%)	Gaps:	167/886 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 KSSE------VPNRELIGTDYVS-KIVAKEKCLLN--GTLLNEQPQGKRIRTLDDLDTDDEG--- 90
            |.||      .|.....||:..| |.:..||.|.|  ..|||::.:..||.....:|.|.|.
Mouse   101 KDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTFRITDKSYIDRDSEYLLQ 165

  Fly    91 -------------EETEIRRDDEYY---KKFRFNLNRDKNLSIYAKREEILAAINAHPVVIIKGE 139
                         |:.:.::.|..|   ::||      |.|..|..::|::..||.|.|.:|.||
Mouse   166 ENEPNLSLDQHLLEDLQRKKTDPRYIEMQRFR------KKLPSYGMQKELVNLINNHQVTVISGE 224

  Fly   140 TGCGKTTQVPQYILDEAYKSGK--YCNIVVTQPRRIAAISIANRVCQER--EWQQNTVCSFQVGL 200
            |||||||||.|:|||...:.||  .|.||.||||||:|||:|.||..||  .........:|:.|
Mouse   225 TGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVATERAESCGNGNSTGYQIRL 289

  Fly   201 HRPNSLEDTRLLYCTTGVLLNNLINNKTLTHYTHIVLDEVHERDQNMDFLLIVVRRLLATNSRHV 265
            ......:...:||||||::|..|.::..|:..:||||||:|||:...|.|:.|::.||...| .:
Mouse   290 QSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRS-DL 353

  Fly   266 KIILMSATIDAKELSDYFTTTNSIP------PVIT------------------------------ 294
            |:||||||::|::.|:||.....|.      ||:.
Mouse   354 KVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYVPDQKEHRSQFKRGFMQG 418

  Fly   295 -TNHRRKHSIEKFYRDQLGSIIWNEEDVGHQQVPEINKHGYRAAVKIIVIID----NMERKAAIQ 354
             .|.:.|...|..|:::     |      ...:.|:......:.|.::.::|    ::...||:
Mouse   419 HVNRQEKEEKEAIYKER-----W------PAYIKELRTRYSASTVDVLQMMDDDKVDLNLIAAL- 471

  Fly   355 SRQSYDEALRY-------GAVLIFLPGIYEIDTMAENL--TCMLENDPNIKVSIVRCFSLMTPEN 410
                    :||       ||:|:||||...|.|:.:.|  ..|.::|   |..|:...|||...|
Mouse   472 --------IRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSD---KFLIIPLHSLMPTVN 525

  Fly   411 QRDVFNPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLAKVKVT--DTASSFSSLRLTWASKAN 473
            |..||...|||.|||::.|||||:|||:.||.||||.  .|:|.|  ||.::.|::...|.||||
Mouse   526 QTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDG--GKIKETHFDTQNNISTMSAEWVSKAN 588

  Fly   474 CRQRAGRVGRLRSGRVYRMVNKHFYQREMPEFGIPEMLRLPLQNSVLKAKVLNMGSPVEILALAL 538
            .:||.||.||::.|..|.:.| ......:.::.:||:||.||:...|:.|:|.:|.....|:..:
Mouse   589 AKQRKGRAGRVQPGHCYHLYN-GLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLM 652

  Fly   539 SPPNLSDIHNTILLLKEVGALYLTVDGIYDPLDGDLTYWGTIMSRLPLDTRQSRLIILGYIFNML 603
            .||:...:..:|..|.|:.||        |..: :||..|..::|||::....::|:.|.:|..|
Mouse   653 DPPSNEAVVLSIKHLMELSAL--------DKQE-ELTPLGVHLARLPVEPHIGKMILFGALFCCL 708

  Fly   604 EEAIIIAAGLSTPGLFAHEGGRSQLGDSFWMHYIFSDGSGSDLVAIWRVYLTYLNIVENGHD-QE 667
            :..:.|||.||....|....|:.::.|:  .....:..:.||       :||.:|..|...: :.
Mouse   709 DPVLTIAASLSFKDPFVIPLGKEKIADA--RRKELAKETRSD-------HLTVVNAFEGWEEAKR 764

  Fly   668 SAIRWAK----RFHVSLRSLKEIHLLVQELRVRCTHLGLIP--FPVNPN-QMMDDREKAIMLKVI 725
            ...|:.|    .:.:|..:|:.:|.:..:........|.:.  .|.:|. .:..|.||  ::|.:
Mouse   765 RGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEK--IIKAV 827

  Fly   726 IAGAFYP-------NYFTRSKESCADTDRNIYQTISGHDPCRTVYFTNFKPAYMGELYTRRIKE- 782
            |....||       |...:.|.....|..:  ..:|.|.....|..|:|.  |...:|..:::.
Mouse   828 ICAGLYPKVAKIRLNLGKKRKMVKVHTKSD--GLVSIHPKSVNVEQTDFH--YNWLIYHLKMRTS 888

  Fly   783 ---LFQEVRIPPENM-----DVTFQEGSQKVFVTFKQDDWI 815
               |:....:.|..:     |::.|:...:..:..  |:||
Mouse   889 SIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAV--DEWI 927

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
spn-E	NP_476741.1	HrpA	113..>697	CDD:224557	204/644 (32%)
spn-E	NP_476741.1	TUDOR	892..1016	CDD:395449
Dhx36	NP_082412.2	Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..193		23/91 (25%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000250\|UniProtKB:D4A2Z8	1..97
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..54
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..44
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	46..98
		DSM (DHX36-specific motif). /evidence=ECO:0000250\|UniProtKB:Q05B79, ECO:0000250\|UniProtKB:Q9H2U1	46..68
		RecA-like domain 1. /evidence=ECO:0000250\|UniProtKB:Q05B79	99..379		106/284 (37%)
		HrpA	188..894	CDD:224557	228/762 (30%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	258..310		19/51 (37%)
		DEAH box. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00541	327..330		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250\|UniProtKB:Q05B79	380..621		75/266 (28%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	491..550		25/61 (41%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	510..521		4/13 (31%)
		WH domain. /evidence=ECO:0000250\|UniProtKB:Q05B79	622..691		24/77 (31%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	631..690		17/67 (25%)
		OB-fold-like subdomains. /evidence=ECO:0000250\|UniProtKB:Q05B79	834..898		12/67 (18%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	842..853		2/10 (20%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	863..893		6/31 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG1643
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D278674at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.820