DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment spn-E and Dhx36

DIOPT Version :9

Sequence 1:	NP_476741.1	Gene:	spn-E / 41919	FlyBaseID:	FBgn0003483	Length:	1434	Species:	Drosophila melanogaster
Sequence 2:	NP_001101148.1	Gene:	Dhx36 / 310461	RGDID:	1308767	Length:	1000	Species:	Rattus norvegicus

Alignment Length:	890	Identity:	254/890 - (28%)
Similarity:	403/890 - (45%)	Gaps:	163/890 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    32 LMATKFKSSE------VPNRELIGTDYVS-KIVAKEKCLLN--GTLLNEQPQGKRIRTLDDLDTD 87
            :.|...|.||      .|.....||:..| |.:..||.|.|  ..|||::.:..||.....:|.|
  Rat    94 VQAKNDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTYRITDKSYIDRD 158

  Fly    88 DE----------GEETEI-------RRDDEYYKKFRFNLNRDKNLSIYAKREEILAAINAHPVVI 135
            .|          |.:.::       :.|..|.:..||.    |.|..|..::|::..||.|.|.:
  Rat   159 SEYLLQQNEPNLGLDQQLLEDLQKKKTDPRYIEMQRFR----KKLPSYGMQKELVNLINNHQVTV 219

  Fly   136 IKGETGCGKTTQVPQYILDEAYKS--GKYCNIVVTQPRRIAAISIANRVCQER--EWQQNTVCSF 196
            |.|||||||||||.|:|||...:.  |..|.||.||||||:|||:|.||..||  .........:
  Rat   220 ISGETGCGKTTQVTQFILDNYIERGIGSACRIVCTQPRRISAISVAERVAAERAESCGNGNSTGY 284

  Fly   197 QVGLHRPNSLEDTRLLYCTTGVLLNNLINNKTLTHYTHIVLDEVHERDQNMDFLLIVVRRLLATN 261
            |:.|......:...:||||||::|..|.::..|:..:||||||:|||:...|.|:.|::.||...
  Rat   285 QIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFR 349

  Fly   262 SRHVKIILMSATIDAKELSDYFTTTNSIP------PVIT-------------------------- 294
            | .:|:||||||::|::.|:||.....|.      ||:.
  Rat   350 S-DLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYFPEQKEHRSQFKRG 413

  Fly   295 -----TNHRRKHSIEKFYRDQLGSIIWNEEDVGHQQVPEINKHGYRAAVKIIVIID----NMERK 350
                 .|.:.|...|..|:::     |      ...:.|:......:.:.::.::|    ::...
  Rat   414 FMQGHVNRQEKEEKEAIYKER-----W------PAYIKELQTRYSASTIDVLEMMDDDKVDLNLI 467

  Fly   351 AAIQSRQSYDEALRY-------GAVLIFLPGIYEIDTMAENL--TCMLENDPNIKVSIVRCFSLM 406
            ||:         :||       ||:|:||||...|.|:.:.|  ..|.::|   :..|:...|||
  Rat   468 AAL---------IRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSD---RFLIIPLHSLM 520

  Fly   407 TPENQRDVFNPPPPGFRKIILTTNIAESSITVPDVSYVIDFCLAKVKVT--DTASSFSSLRLTWA 469
            ...||..||...|||.|||::.|||||:|||:.||.||||.  .|:|.|  ||.::.|::...|.
  Rat   521 PTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDG--GKIKETHFDTQNNISTMSAEWV 583

  Fly   470 SKANCRQRAGRVGRLRSGRVYRMVNKHFYQREMPEFGIPEMLRLPLQNSVLKAKVLNMGSPVEIL 534
            ||||.:||.||.||::.|..|.:.| ......:.::.:||:||.||:...|:.|:|.:|.....|
  Rat   584 SKANAKQRKGRAGRVQPGHCYHLYN-GLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFL 647

  Fly   535 ALALSPPNLSDIHNTILLLKEVGALYLTVDGIYDPLDGDLTYWGTIMSRLPLDTRQSRLIILGYI 599
            :..:.||:...:..:|..|.|:.||        |..: :||..|..::|||::....::|:.|.:
  Rat   648 SRLMDPPSDEAVVLSIKHLMELSAL--------DKQE-ELTPLGVHLARLPVEPHIGKMILFGAL 703

  Fly   600 FNMLEEAIIIAAGLSTPGLFAHEGGRSQLGDSFWMHYIFSDGSGSDLVAIWRVYLTYLNIVENGH 664
            |..|:..:.|||.||....|....|:.::.|:  .....:..:.||       :||.:|..|...
  Rat   704 FCCLDPVLTIAASLSFKDPFVIPLGKEKIADA--RRKELAKETRSD-------HLTVVNAFEGWE 759

  Fly   665 D-QESAIRWAK----RFHVSLRSLKEIHLLVQELRVRCTHLGLIP--FPVNPN-QMMDDREKAIM 721
            : :....|:.|    .:.:|..:|:.:|.:..:........|.:.  .|.:|. .:..|.||  :
  Rat   760 EAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEK--I 822

  Fly   722 LKVIIAGAFYP-------NYFTRSKESCADTDRNIYQTISGHDPCRTVYFTNFKPAYMGELYTRR 779
            :|.:|....||       |...:.|.....|..:  ..:|.|.....|..|:|.  |...:|..:
  Rat   823 IKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSD--GLVSIHPKSVNVEQTDFH--YNWLIYHLK 883

  Fly   780 IKE----LFQEVRIPPENM-----DVTFQEGSQKVFVTFKQDDWI 815
            ::.    |:....:.|..:     |::.|:...:..:..  |:||
  Rat   884 MRTSSIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAV--DEWI 926

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
spn-E	NP_476741.1	HrpA	113..>697	CDD:224557	201/644 (31%)
spn-E	NP_476741.1	TUDOR	892..1016	CDD:395449
Dhx36	NP_001101148.1	HrpA	187..893	CDD:224557	225/760 (30%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	257..309		19/51 (37%)
		DEAH box. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00541	326..329		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250\|UniProtKB:Q05B79	379..620		73/266 (27%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	490..549		24/61 (39%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	509..520		3/13 (23%)
		WH domain. /evidence=ECO:0000250\|UniProtKB:Q05B79	621..690		24/77 (31%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	630..689		17/67 (25%)
		OB-fold-like subdomains. /evidence=ECO:0000250\|UniProtKB:Q05B79	833..897		12/67 (18%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	841..852		2/10 (20%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	862..892		6/31 (19%)
		Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..192		24/97 (25%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000269\|PubMed:23651854	1..96		0/1 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..53
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..43
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	45..97		0/2 (0%)
		DSM (DHX36-specific motif). /evidence=ECO:0000250\|UniProtKB:Q05B79, ECO:0000250\|UniProtKB:Q9H2U1	45..67
		RecA-like domain 1. /evidence=ECO:0000250\|UniProtKB:Q05B79	98..378		105/284 (37%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG1643
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D278674at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.820