Protein Alignment Atg4b and ATG4

DIOPT Version :9

• Sequence 1: NP_650452.1
• Gene: Atg4b / 41868
• FlyBaseID: FBgn0038325
• Length: 668
• Species: Drosophila melanogaster
• Sequence 2: NP_014176.2
• Gene: ATG4 / 855498
• SGDID: S000005167
• Length: 494
• Species: Saccharomyces cerevisiae
• Alignment Length: 327
• Identity: 89/327 - (27%)
• Similarity: 135/327 - (41%)
• Gaps: 72/327 - (22%)

  Fly   266 FRRDFYSRIWMTYRREFP-----------------------------IMNGSNYTSDCGWGCMLR 301
            |..|..||:..|||..|.                             |.|...:.:|.|||||:|
Yeast    86 FLLDVQSRVNFTYRTRFVPIARAPDGPSPLSLNLLVRTNPISTIEDYIANPDCFNTDIGWGCMIR 150

  Fly   302 SGQMLFAQGLICHFLGRSWRYDSESQLHSTYEDNMHKKIVKWFGDSSSKSSPFSIHALVRLGEHL 366
            :||.|....|....|||.:|.:....|      ....|.|.||.|  :..:|||:|..|..|..|
Yeast   151 TGQSLLGNALQILHLGRDFRVNGNESL------ERESKFVNWFND--TPEAPFSLHNFVSAGTEL 207

  Fly   367 G-KKPGDWYGPASVSYLLKHALEHAAQENADFDNISVYVAKDCTIYLQDIEDQCSIPEPAPKPHV 430
            . |:||:|:|||:.:..::..               :|...:|.|      |.|.:...:...:
Yeast   208 SDKRPGEWFGPAATARSIQSL---------------IYGFPECGI------DDCIVSVSSGDIY- 250

  Fly   431 PWQQAKRPQAETTKTEQQQHWKSLIVLIPLRLGSDKLNPVYAHCLKLLLSTEHCLGILGGKPKHS 495
                    :.|..|...:.....::.|:.::||.:.:|..|...:..:||:...:||.||:|..|
Yeast   251 --------ENEVEKVFAENPNSRILFLLGVKLGINAVNESYRESICGILSSTQSVGIAGGRPSSS 307

  Fly   496 LYFVGFQEDRLIHLDPHYCQEMVDVNQENFSLHSFHCKSPRKLKASKMDPSCCIGFYCATKSDFD 560
            |||.|:|.:..:|.|||..|..|    |:..:.|.|.....||:.|:||||..||.....:.|:.
Yeast   308 LYFFGYQGNEFLHFDPHIPQPAV----EDSFVESCHTSKFGKLQLSEMDPSMLIGILIKGEKDWQ 368

  Fly   561 NF 562
            .:
Yeast   369 QW 370

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Atg4b	NP_650452.1	Peptidase_C54	264..565	CDD:281417	89/327 (27%)
ATG4	NP_014176.2	Peptidase_C54	84..371	CDD:397471	89/327 (27%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157343916
Domainoid	1	1.000	121	1.000	Domainoid score	I1249
eggNOG	1	0.900	-	-		E1_KOG2674
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000466
OrthoInspector	1	1.000	-	-		otm46790
orthoMCL	1	0.900	-	-		OOG6_100501
Panther	1	1.100	-	-	LDO	PTHR22624
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R109
SonicParanoid	1	1.000	-	-		X289
TreeFam	1	0.960	-	-
	12	11.730