Protein Alignment Atg4b and ATG4D

DIOPT Version :9

• Sequence 1: NP_650452.1
• Gene: Atg4b / 41868
• FlyBaseID: FBgn0038325
• Length: 668
• Species: Drosophila melanogaster
• Sequence 2: NP_116274.3
• Gene: ATG4D / 84971
• HGNCID: 20789
• Length: 474
• Species: Homo sapiens
• Alignment Length: 629
• Identity: 201/629 - (31%)
• Similarity: 277/629 - (44%)
• Gaps: 214/629 - (34%)

  Fly    69 PRVPSPSPVSTSGANLNLKTENAATATPQRKISTSSRFMNAFSQLYGGGNSGPSCGHVEQHNEEP 133
            ||.|.|:.:..|||                                    |||:.|.......||
Human    31 PRGPDPNGLGPSGA------------------------------------SGPALGSPGAGPSEP 59

  Fly   134 GDLSANRTPTKGMESKLVAMWHNVKYGWSGKMRQTSFSKEQPVWLLGRCYHRRFTPPVSMESSIT 198
            .::..       .::|.:..|:||||||..|.| |||||...:.|.||.|  ||
Human    60 DEVDK-------FKAKFLTAWNNVKYGWVVKSR-TSFSKISSIHLCGRRY--RF----------- 103

  Fly   199 ELPSGADTTPDNATSAFDSIQATSTSTSLYPALNPQQIDEIVVPQELGMDAVENQVGEQPWEEGI 263
                                                                       ..|..|
Human   104 -----------------------------------------------------------EGEGDI 109

  Fly   264 EGFRRDFYSRIWMTYRREFPIMNGSNYTSDCGWGCMLRSGQMLFAQGLICHFLGRSWRY------ 322
            :.|:|||.||:|:||||:||.:.|...|||||||||||||||:.||||:.|||.|.|.:
Human   110 QRFQRDFVSRLWLTYRRDFPPLPGGCLTSDCGWGCMLRSGQMMLAQGLLLHFLPRDWTWAEGMGL 174

  Fly   323 --------DSESQLH---------------STYEDNMHKKIVKWFGDSSSKSSPFSIHALVRLGE 364
                    .|.|:.|               ...::..|::||.||.|  ...:||.:|.||.||:
Human   175 GPPELSGSASPSRYHGPARWMPPRWAQGAPELEQERRHRQIVSWFAD--HPRAPFGLHRLVELGQ 237

  Fly   365 HLGKKPGDWYGPASVSYLLKHALEHAAQENADFDNISVYVAKDCTIYLQDIEDQCSIPEPAPKPH 429
            ..|||.||||||:.|:::|:.|:|..    :|...:.|||::|||:|..|:....:.|:|..:
Human   238 SSGKKAGDWYGPSLVAHILRKAVESC----SDVTRLVVYVSQDCTVYKADVARLVARPDPTAE-- 296

  Fly   430 VPWQQAKRPQAETTKTEQQQHWKSLIVLIPLRLGSDKLNPVYAHCLKLLLSTEHCLGILGGKPKH 494
                                 |||:::|:|:|||.:.|||||..|:|.||..|.||||:||||:|
Human   297 ---------------------WKSVVILVPVRLGGETLNPVYVPCVKELLRCELCLGIMGGKPRH 340

  Fly   495 SLYFVGFQEDRLIHLDPHYCQEMVDVNQENFSLHSFHCKSPRKLKASKMDPSCCIGFYCATKSDF 559
            ||||:|:|:|.|::|||||||..|||:|.:|.|.||||.||||:..:||||||.:|||...:.:|
Human   341 SLYFIGYQDDFLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEF 405

  Fly   560 DNFMESVQLYLHPMRCASGATVDKAAGSHHTTPQSSHQTEQTEMNYPLFSFSRGRCMDHERDEMS 624
            :.....:...|                      .||..||:    ||:|:.:.|...||..|::.
Human   406 ETLCSELTRVL----------------------SSSSATER----YPMFTLAEGHAQDHSLDDLC 444

  Fly   625 DSLYKPLIKQVASLAQELGAQLAPPQHGDEHDQDDSESEEFVLL 668
            ..|.:|.::.              |:.|.........||:||.|
Human   445 SQLAQPTLRL--------------PRTGRLLRAKRPSSEDFVFL 474

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Atg4b	NP_650452.1	Peptidase_C54	264..565	CDD:281417	144/329 (44%)
ATG4D	NP_116274.3	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..60		12/64 (19%)
		Cryptic mitochondrial signal peptide	64..103		19/48 (40%)
		Peptidase_C54	110..411	CDD:281417	144/329 (44%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165147541
Domainoid	1	1.000	295	1.000	Domainoid score	I1482
eggNOG	1	0.900	-	-		E1_KOG2674
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H13156
Inparanoid	1	1.050	354	1.000	Inparanoid score	I2250
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D332531at33208
OrthoFinder	1	1.000	-	-		FOG0000466
OrthoInspector	1	1.000	-	-		otm41297
orthoMCL	1	0.900	-	-		OOG6_100501
Panther	1	1.100	-	-	LDO	PTHR22624
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R109
SonicParanoid	1	1.000	-	-		X289
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	15	14.790