Protein Alignment Atg4b and atg4da

DIOPT Version :9

• Sequence 1: NP_650452.1
• Gene: Atg4b / 41868
• FlyBaseID: FBgn0038325
• Length: 668
• Species: Drosophila melanogaster
• Sequence 2: XP_009292711.1
• Gene: atg4da / 795933
• ZFINID: ZDB-GENE-041111-102
• Length: 485
• Species: Danio rerio
• Alignment Length: 639
• Identity: 189/639 - (29%)
• Similarity: 283/639 - (44%)
• Gaps: 238/639 - (37%)

  Fly   108 NAFSQLYGGG--------NSGPSCGH------VEQHNEEPGDLSANRTPTKGMESKLVAMWHNVK 158
            :|...:.|||        :|....||      :.:.:.||.::..       :::|.::.|:|||
Zfish     7 SAVQYIVGGGAHEDKASASSKRHLGHGAVPDGIREGSGEPDEVDR-------LKAKFMSAWNNVK 64

  Fly   159 YGWSGKMRQTSFSKEQPVWLLGRCYHRRFTPPVSMESSITELPSGADTTPDNATSAFDSIQATST 223
            |||:.|.: |||:|..|:.|||:.:                              .|::
Zfish    65 YGWTVKSK-TSFNKSSPLILLGQSF------------------------------LFNN------ 92

  Fly   224 STSLYPALNPQQIDEIVVPQELGMDAVENQVGEQPWEEGIEGFRRDFYSRIWMTYRREFPIMNGS 288
                                                |:.:|.||:.|.|.:|:|||||||.::||
Zfish    93 ------------------------------------EDEVERFRQTFVSCVWLTYRREFPQLDGS 121

  Fly   289 NYTSDCGWGCMLRSGQMLFAQGLICHFLGRSWRYDSESQLHSTYE-------------------- 333
            :.|:|||||||||||||:.||||:.|.:...||:   |..|:..:
Zfish   122 SLTTDCGWGCMLRSGQMMLAQGLLLHLMPTDWRW---SDCHALTDVDFEVLKPRSPSRPAGMSMP 183

  Fly   334 ---------------------------------------DNMHKKIVKWFGDSSSKSSPFSIHAL 359
                                                   |.:|:|:|..|||  ..|:||.:|.|
Zfish   184 SFSSSWSSSIPQINPSPGITEAHRRAPARCPSASPDPQVDALHRKVVSCFGD--HPSAPFGVHQL 246

  Fly   360 VRLGEHLGKKPGDWYGPASVSYLLKHALEHAAQENADFDNISVYVAKDCTIYLQDIEDQCSIPEP 424
            |.||:..||:.||||||:.|:::|:.|:..|    |:|::::||||:|||:|.:|:...|.
Zfish   247 VELGKESGKRAGDWYGPSVVAHMLRKAVARA----AEFEDLAVYVAQDCTVYKEDVMSLCE---- 303

  Fly   425 APKPHVPWQQAKRPQAETTKTEQQQHWKSLIVLIPLRLGSDKLNPVYAHCLKLLLSTEHCLGILG 489
                                 .....|||:::|:|:|||.:.|||.|..|:|.:|..:.|:||:|
Zfish   304 ---------------------SSGVGWKSVVILVPVRLGGESLNPSYIECVKNILKLKCCIGIIG 347

  Fly   490 GKPKHSLYFVGFQEDRLIHLDPHYCQEMVDVNQENFSLHSFHCKSPRKLKASKMDPSCCIGFYCA 554
            |||||||:|||||:::|::|||||||.:|||.|.||||.||||.||||:..|:|||||.||.|..
Zfish   348 GKPKHSLFFVGFQDEQLLYLDPHYCQPVVDVTQANFSLESFHCNSPRKMNFSRMDPSCTIGLYAR 412

  Fly   555 TKSDFDNFMESVQLYLHPMRCASGATVDKAAGSHHTTPQSSHQTEQTEMNYPLFSFSRGRCMDHE 619
            :|:||::...:|                            |.....::..||:|:|..||
Zfish   413 SKTDFESLCTAV----------------------------SEALSSSKEKYPIFTFVEGR----- 444

  Fly   620 RDEMSDSLYKPLIKQVASLAQELGAQLAPPQH-----GDEHDQDDSESEEFVLL 668
                         .|:..:....|..:..|.|     ....:.....::|||||
Zfish   445 -------------GQIYGMEGPSGGSVDAPAHIFTCSRLSRNNKRGSTDEFVLL 485

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Atg4b	NP_650452.1	Peptidase_C54	264..565	CDD:281417	144/359 (40%)
atg4da	XP_009292711.1	Peptidase_C54	97..422	CDD:308812	144/358 (40%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170581283
Domainoid	1	1.000	282	1.000	Domainoid score	I1615
eggNOG	1	0.900	-	-		E1_KOG2674
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	328	1.000	Inparanoid score	I2444
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D332531at33208
OrthoFinder	1	1.000	-	-		FOG0000466
OrthoInspector	1	1.000	-	-		otm25992
orthoMCL	1	0.900	-	-		OOG6_100501
Panther	1	1.100	-	-	LDO	PTHR22624
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R109
SonicParanoid	1	1.000	-	-		X289
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.830