Protein Alignment Atg4b and atg4d

DIOPT Version :9

• Sequence 1: NP_650452.1
• Gene: Atg4b / 41868
• FlyBaseID: FBgn0038325
• Length: 668
• Species: Drosophila melanogaster
• Sequence 2: NP_001039246.1
• Gene: atg4d / 734111
• XenbaseID: XB-GENE-1016717
• Length: 470
• Species: Xenopus tropicalis
• Alignment Length: 611
• Identity: 196/611 - (32%)
• Similarity: 281/611 - (45%)
• Gaps: 190/611 - (31%)

  Fly   107 MNAFSQL---YGGGNSG--------PSCGHVEQHNEEPGDLSA--NRTPTKGMESKLVAMWHNVK 158
            ||:.|.|   ||.....        || ||.||......|::|  .......::|||:..|:|||
 Frog     1 MNSVSPLATQYGSPKGSQQTENRRTPS-GHHEQRRVGYQDVTAEGETDEVDKLKSKLLTAWNNVK 64

  Fly   159 YGWSGKMRQTSFSKEQPVWLLGRCYHRRFTPPVSMESSITELPSGADTTPDNATSAFDSIQATST 223
            ||||.||: |:||:..||:|||..|:.|.
 Frog    65 YGWSVKMK-TTFSRSAPVYLLGERYYFRL------------------------------------ 92

  Fly   224 STSLYPALNPQQIDEIVVPQELGMDAVENQVGEQPWEEGIEGFRRDFYSRIWMTYRREFPIMNGS 288
                                                ::.|:.|::||.||:|:||||:||.:.|:
 Frog    93 ------------------------------------DDEIDRFQKDFVSRVWLTYRRDFPALEGT 121

  Fly   289 NYTSDCGWGCMLRSGQMLFAQGLICHFLGRSWRYD------------------------------ 323
            ..|:|||||||:||||||.||||:.|.|.|.|.:.
 Frog   122 ALTTDCGWGCMIRSGQMLLAQGLLLHLLSREWTWSEALYTHFVEMEPIRSSSPSSMPLSLATDHS 186

  Fly   324 ---SESQLH---STYEDNMHKKIVKWFGDSSSKSSPFSIHALVRLGEHLGKKPGDWYGPASVSYL 382
               |:.|.|   :.|...:|:.||.||.|.:  |:||.:|.:|.||...||:.||||||:.|:::
 Frog   187 GRHSQPQTHCSRAPYGGEVHQNIVSWFSDHA--SAPFGLHRMVALGSIFGKRAGDWYGPSIVAHI 249

  Fly   383 LKHALEHAAQENADFDNISVYVAKDCTIYLQDIEDQCSIPEPAPKPHVPWQQAKRPQAETTKTEQ 447
            :|.|:|    .:::..::||||::|||:|..|||...:              .:.|..:|::..
 Frog   250 IKKAIE----SSSEVPDLSVYVSQDCTVYKADIEQLFA--------------GEVPHTDTSRGA- 295

  Fly   448 QQHWKSLIVLIPLRLGSDKLNPVYAHCLKLLLSTEHCLGILGGKPKHSLYFVGFQEDRLIHLDPH 512
               .|::|:|:|.|||.:..||||.||||..|....||||:||||||||||:|:|::.|::||||
 Frog   296 ---GKAVIILVPARLGGETFNPVYKHCLKEFLRMPSCLGIIGGKPKHSLYFIGYQDNYLLYLDPH 357

  Fly   513 YCQEMVDVNQENFSLHSFHCKSPRKLKASKMDPSCCIGFYCATKSDFDNFMESVQLYLHPMRCAS 577
            |||..:|.:::||.|.||||.:||||..::|||||...||...:.||....|.:...||
 Frog   358 YCQPYIDTSRDNFPLESFHCNAPRKLSITRMDPSCTFAFYAKNRDDFGKLCEHLSKVLH------ 416

  Fly   578 GATVDKAAGSHHTTPQSSHQTEQTEMNYPLFSFSRGRCMDHERDEMSDSLYKPLIKQVASLAQEL 642
                                :.|.|..||:||.|.|:..::...|...|...|      .:.::.
 Frog   417 --------------------SPQAEEKYPIFSISEGQAQEYASAEGQQSSSHP------PVCRKK 455

  Fly   643 GAQLAPPQHGDEHDQDDSESEEFVLL 668
            |..:..|           .|:||..|
 Frog   456 GPMVKRP-----------SSDEFEFL 470

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Atg4b	NP_650452.1	Peptidase_C54	264..565	CDD:281417	138/336 (41%)
atg4d	NP_001039246.1	Peptidase_C54	97..409	CDD:308812	138/335 (41%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	288	1.000	Domainoid score	I1548
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H13156
Inparanoid	1	1.050	351	1.000	Inparanoid score	I2218
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D332531at33208
OrthoFinder	1	1.000	-	-		FOG0000466
OrthoInspector	1	1.000	-	-		otm48501
Panther	1	1.100	-	-	LDO	PTHR22624
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R109
SonicParanoid	1	1.000	-	-		X289
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	11	11.100